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An Investigation into the In-Vitro Titration of Cobra and Daboia Antivenins
Summary
- 1.The hemolysin fraction of cobra venom can be completely eliminated by treatment with alkali.
- 2.The determination of the minimal lethal dose of hemolysin showed that it was almost innocuous when administered intra-muscularly into guinea-pigs but was definitely toxic when injected intra-cardially or intra-peritoneally.
- 3.Cobra neurotoxin was used for the in-vitro titration of its corresponding antibody, but the results did not exactly correspond to the protective power of antivenom sera. The anti-hemolysin was linked with the anti-neurotoxin and interefered with the neurotoxin + anti-neurotoxin interaction.
- 4.It has, so far, not been found possible to fractionate daboia venom.
- 5.Daboia anti-hemolysin titration or modified Nagler's reaction can be used as preliminary in-vitro tests before the final in-vivo titration is undertaken.