In Am J Trop Med Hyg 74(2): 205–210 by Menard and others, the authors wished to verify some results by sequencing, and the manuscript was published prematurely. According to sequencing, the authors found some discordance with PCR RFLP analysis.
Some modifications should be made in text, namely:
In Results section
— In mutant alleles of the pfmdr1 and pfcrt genes paragraph, read:
Mutant alleles of the pfcrt gene were found in one third of samples (114/172).
— In mutant alleles of the dhfr and dhps genes paragraph, read:
The most prevalent haplotype carried three mutant alleles: 51I, 59R, and 108N.
Significant correlations were found in wild-type and mutant alleles between codons 51 and 59 (p < 10−6), codons 51 and 108 (p = 0.005) and codons 59 and 108 (p = 0.0005).
The triple dhfr variant (51I, 59R and 108N) was found in combination with the dhps variant 436A in parasites from 41.6% of the individuals, with dhps variant 437G in parasites from 3.4% of the individuals and with the dhps variant 540E in parasites from 2.2% of the individuals.
In discussion section, read: Of the 167 P. falciparum isolates examined for the dhfr gene, only 12 carried the wild-type allele (7.2%) and there was a high frequency (57.5%) of triple mutants (51I, 59R, 108N).
and in tables, namely:
In Table 1: Read for codon 75 in pfcrt gene:
— wild allele: count 96, frequency 53.6% (95%IC: 46.3–60.8)
— mixed alleles count 10, frequency 5.6% (95%IC: 2.8–9.7)
— mutant allele: count 73, frequency 40.8% (95%IC: 33.8–48.1)
In Table 2: Read for codon 50 in dhfr gene:
— wild allele: count 171, frequency 100% (95%IC: 98–100)
— mixed alleles count 0, frequency 0%
— mutant allele: count 0, frequency 0%
In Table 3: Frequency of deduced haplotype profiles for the pfcrt gene of Plasmodium falciparum isolates from Bangui (CAR) in 2004 are CIET 45%, CMNK 33.3%, CMNT 12.3%, CIEK 4.7%, SIET 2.3% SIEK 1.2%, SMET 0.6% and CINT 0.6%
In Table 4: Frequency of deduced haplotype profiles for the dhfr gene of Plasmodium falciparum isolates from Bangui (CAR) in 2004 are CIRNI 57.5%, CIRSI 9.6%, CNCNI 8.4%, CNRNI 7.8%, CNCSI 7.2%, CNRSI 3.0%, CICNI 3.0%, CICSI 2.4%, CNCTI 0.6%, CIRSL 0.6% and CIRNL 0.6%