Authors’ addresses: Chairat Tantrawatpan, Chaisiri Wongkham, and Sopit Wongkham, Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. Wanchai Maleewong and Pewpan M. Intapan, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. Kunio Nakashima, Faculty of Human Health, Tokai Gakuen University, Nakahira 2-901, Tenpaku-ku, Nagoya, Aichi, Japan.
Acknowledgments: We thank Dr. Nimit Morakote (Faculty of Medicine, Chiang Mai University, Chang Mai, Thailand) for providing trichinosis sera and Dr. Mark Roselieb for improving the English language presentation of the manuscript.
Financial support: This research was supported by the Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program (grant no. PHD/0155/2542 to Chairat Tantrawatpan) and Wanchai Malee-wong and a grant from Khon Kaen University.
Mas-Coma S, Bargues MD, Esteban JG, 1998. Human fasciolosis. Dalton JP, ed. Fasciolosis. Wallingford, United Kingdom: CABI Publishing, 411–434.
Andrews SJ, 1998. The life cycle of Fasciola hepatica. Dalton JP, ed. Fasciolosis. Wallingford, United Kingdom: CABI Publishing, 1–29.
Maleewong W, Wongkham C, Intapan PM, Pipitgool V, 1999. Fasciola gigantica-specific antigens: purification by a continuous-elution method and its evaluation for the diagnosis of human fascioliasis. Am J Trop Med Hyg 61 :648–651.
Grams R, Vichasri-Grams S, Sobhon P, Upatham ES, Viyanant V, 2001. Molecular cloning and characterization of cathepsin L encoding genes from Fasciola gigantica. Parasitol Int 50 :105–114.
O’Neill SM, Parkinson M, Strauss W, Angles R, Dalton JP, 1998. Immunodiagnosis of Fasciola hepatica infection (fascioliasis) in a human population in the Bolivian Altiplano using purified cathepsin L cysteine proteinase. Am J Trop Med Hyg 58 :417–423.
O’Neill SM, Parkinson M, Dowd AJ, Strauss W, Angles R, Dalton JP, 1999. Immunodiagnosis of human fascioliasis using recombinant Fasciola hepatica cathepsin L1 cysteine proteinase. Am J Trop Med Hyg 60 :749–751.
Strauss W, O’Neill SM, Parkinson M, Angles R, Dalton JP, 1999. Diagnosis of human fascioliasis: detection of anti-cathepsin L antibodies in blood samples collected on filter paper. Am J Trop Med Hyg 60 :746–748.
Carnevale S, Rodriguez MI, Guarnera EA, Carmona C, Tanos T, Angel SO, 2001. Immunodiagnosis of fasciolosis using recombinant procathepsin L cystein proteinase. Diagn Microbiol Infect Dis 41 :43–49.
Cornelissen JB, Gaasenbeek CP, Borgsteede FH, Holland WG, Harmsen MM, Boersma WJ, 2001. Early immunodiagnosis of fasciolosis in ruminants using recombinant Fasciola hepatica cathepsin L-like protease. Int J Parasitol 31 :728–737.
Rokni MB, Massoud J, O’Neill SM, Parkinson M, Dalton JP, 2002. Diagnosis of human fasciolosis in the Gilan province of northern Iran: application of cathepsin L-ELISA. Diagn Microbiol Infect Dis 44 :175–179.
Erdman DD, 1981. Clinical comparison of ethyl acetate and di-ethyl ether in the formalin-ether sedimentation technique. J Clin Microbiol 14 :483–485.
Chomczynski P, Sacchi N, 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162 :156–159.
Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 :248–254.
Towbin H, Staehelin T, Gordon J, 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 76 :4350–4354.
Ikeda T, 1998. Cystatin capture enzyme-linked immunosorbent assay for immunodiagnosis of human paragonimiasis and fascioliasis. Am J Trop Med Hyg 59 :286–290.
Anastasi A, Brown MA, Kembhavi AA, Nicklin MJ, Sayers CA, Sunter DC, Barrett AJ, 1983. Cystatin, a protein inhibitor of cysteine proteinases. Improved purification from egg white, characterization, and detection in chicken serum. Biochem J 211 :129–138.