By Everard L. Napier, M.R.C.S., L.R.C.P. (Lond.). In charge Kala-azar research, Calcutta School of Tropical Medicine. Second edition. 185 pages of text with 15 charts in the text, 18 plates, and an appendix of references to literature, author index and subject index. Oxford University Press. London, Bombay, Calcutta, Madras, 1927
Preparation of Antigens 561; Preparation of Sera 567; Preparation of Erythrocytes 568; Chemical Reagents 569; Technique of HA and HI Tests 570; HA Titrations 571; HI Tests 572; References 573
During the past few years, the methods for conducting hemagglutination (HA) and hemagglutination-inhibition (HI) tests with arthropod-borne (arbor) viruses have undergone a number of modifications, directed toward simplification and improvement as well as extension of the tests to include a larger number of arbor viruses. Accordingly, it seems desirable at this time to bring together, in one publication rather than in a series of papers, a description of the procedures currently in use in The Rockefeller Foundation Virus Laboratories. Some of the procedures were initially developed elsewhere; of those developed in this laboratory, most aspects have not been previously reported.
It was stated by Hallauer (1946) that he had obtained a hemagglutinating antigen from viscerotropic but not from neurotropic strains of yellow fever.