In response to the letter by Schoyer, in which he describes his previous studies on vertical transmission of dengue-1 virus,1 we were aware of his paper, but only had access to an abstract of it on Medline. Our paper reported, for the first time, the transmission of dengue-3 virus through multiple generations of Aedes aegypti,2 whereas Shroyer reported the transmission of the dengue-1 virus through successive generations of Ae. albopictus. Our group had previously reported the transovarial transmission of dengue-3 virus by Ae. aegypti.3 It is likely that biological differences in these two virus-mosquito pairings are not significant. In our study areas, Ae. albopictus is not common and dengue-1 virus does not circulate. I hope that our paper will provide new information on virus-mosquito interactions in dengue that is especially relevant to different pathogenicities of various dengue virus strains and various Aedes host systems. Moreover, the major focus of our paper was the potential epidemiologic significance of vertical transmission of dengue-3 virus through seven generations of Ae. aegypti, not a priority claim.
Shroyer DA, 1990. Vertical maintenance of dengue-1 virus in sequential generations of Aedes albopictus. J Am Mosq Control Assoc 6 :312–314.
Joshi V, Mourya DT, Sharma RC, 2002. Persistence of dengue-3 virus through transovarial transmission passage in successive generations of Aedes aegypti mosquitoes. Am J Trop Med Hyg 67 :158–161.
Joshi V, Singhi M, Chaudhary RC, 1996. Transovarial transmission of dengue 3 virus by Aedes aegypti. Trans R Soc Trop Med Hyg 90 :643–644.