Genetic diversity of Plasmodium falciparum field samples from an isolated Colombian village.

Diana GómezLaboratory of Biochemistry, Instituto Nacional de Salud, Bogota, Colombia.

Search for other papers by Diana Gómez in
Current site
Google Scholar
PubMed
Close
,
Jacqueline ChaparroLaboratory of Biochemistry, Instituto Nacional de Salud, Bogota, Colombia.

Search for other papers by Jacqueline Chaparro in
Current site
Google Scholar
PubMed
Close
,
Claudia RubianoLaboratory of Biochemistry, Instituto Nacional de Salud, Bogota, Colombia.

Search for other papers by Claudia Rubiano in
Current site
Google Scholar
PubMed
Close
,
María Orfa RojasLaboratory of Biochemistry, Instituto Nacional de Salud, Bogota, Colombia.

Search for other papers by María Orfa Rojas in
Current site
Google Scholar
PubMed
Close
, and
Moises WassermanLaboratory of Biochemistry, Instituto Nacional de Salud, Bogota, Colombia.

Search for other papers by Moises Wasserman in
Current site
Google Scholar
PubMed
Close

Colombian field isolates of Plasmodium falciparum were analyzed for genetic diversity. Fifty-three samples were collected as thick smears from patients living in Panguí, an isolated area with low migration. While the samples were being collected, Panguí was experiencing an epidemic outbreak of malaria. The samples were typified using nested polymerase chain reaction (PCR) amplification of block 2 of the merozoite surface protein 1 (MSP1) gene and nested PCR with mutation-specific primers for position 108 of the dihydrofolate reductase enzyme gene. The results for the circulating population of parasites in Panguí show low diversity--four allelic forms--using MSP1 as a marker, a fact that contrasts with data reported for certain Asian and African zones. A high percentage of mixed infections was observed, as was high complexity of the infection. No differential distributions were found for any allelic type.

Save