Genetic complexity of Plasmodium falciparum gametocytes isolated from the peripheral blood of treated Gambian children.

Colin J SutherlandDepartment of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk

Search for other papers by Colin J Sutherland in
Current site
Google Scholar
PubMed
Close
,
Ali AllouecheDepartment of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk

Search for other papers by Ali Alloueche in
Current site
Google Scholar
PubMed
Close
,
Louisa McRobertDepartment of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk

Search for other papers by Louisa McRobert in
Current site
Google Scholar
PubMed
Close
,
Rosalynn OrdDepartment of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk

Search for other papers by Rosalynn Ord in
Current site
Google Scholar
PubMed
Close
,
Jamie LeggatDepartment of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk

Search for other papers by Jamie Leggat in
Current site
Google Scholar
PubMed
Close
,
Georges SnounouDepartment of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk

Search for other papers by Georges Snounou in
Current site
Google Scholar
PubMed
Close
,
Margaret PinderDepartment of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk

Search for other papers by Margaret Pinder in
Current site
Google Scholar
PubMed
Close
, and
Geoffrey A T TargettDepartment of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk

Search for other papers by Geoffrey A T Targett in
Current site
Google Scholar
PubMed
Close

The genetic complexity of Plasmodium falciparum gametocytes isolated from Gambian children participating in a controlled trial of anti-malarial therapy was investigated. RNA and DNA were prepared from gametocyte-positive blood, which was also used in transmission experiments with Anopheles gambiae mosquitoes. Amplification by a reverse transcriptase-polymerase chain reaction (RT-PCR) of transcripts from the genes for the ring-infected erythrocyte surface antigen and the 16-kD antigen, which exhibit asexual and sexual stage-specific expression, was used to identify 30 post-treatment gametocyte isolates in which trophozoites persisted below the threshold of detection by microscopy. These included isolates from children who received sulfadoxine/pyrimethamine plus artesunate. Twenty-nine gametocyte-positive isolates that were free of subpatent trophozoites were examined further by PCR amplification of polymorphic genomic loci. We estimate that an average minimum of 2.3 genotypes occurred in these gametocyte-only isolates, and many of these were shown to be infective to mosquitoes. Thus, meiotic recombination between different genotypes is predicted to be a common event in this study area.

Save