Short report: possible Cryptosporidium muris infection in humans.

T KatsumataDepartment of Parasitology, Institute of Tropical Medicine, Nagasaki University, Japan.

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D HoseaDepartment of Parasitology, Institute of Tropical Medicine, Nagasaki University, Japan.

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I G RanuhDepartment of Parasitology, Institute of Tropical Medicine, Nagasaki University, Japan.

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S UgaDepartment of Parasitology, Institute of Tropical Medicine, Nagasaki University, Japan.

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T YanagiDepartment of Parasitology, Institute of Tropical Medicine, Nagasaki University, Japan.

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S KohnoDepartment of Parasitology, Institute of Tropical Medicine, Nagasaki University, Japan.

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Oocysts of cryptosporidia whose morphology resembled that of Cryptosporidium muris were found in the stool of 2 healthy girls in Surabaya, Indonesia. The oocysts were predominantly oval and measured 7.75+/-0.17 x 5.55+/-0.13 microm (mean+/-SD). The number of oocysts excreted were more than 10(5) per gram of stool. The oocysts were well stained with fluorescein-conjugated monoclonal antibody to Cryptosporidium. The specimens from both girls containing the oocysts showed a positive result by the polymerase chain reaction (PCR) using primers specific for the genus Cryptosporidium, but a negative result by the PCR using primers specific for C. parvum. The 2 girls passed oocysts for 5 and 6 days, respectively. They did not complain of any symptoms during the passage of oocysts.

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