Department of Microbiology Harvard School of Public Health, Laboratory of Microbiology and Pathology, Department of Health and Home Affairs Queensland, Angell Memorial Hospital, and the Diagnostic Laboratory, Massachusetts Department of Public Health, Boston, Massachusetts, Australia
A modified method for the extraction of ESS from leptospirae has been developed which yields preparations stable for at least 41 weeks at 4°C. ESS antibody apparently deteriorated on storage at -20°C. for more than 1 to 1.5 years. Good correlation between the SEA and the agglutination-lysis tests were obtained with fresh sera. Unsatisfactory results were obtained with sera shipped from Australia. About 8 per cent of sera sent to the Massachusetts Department of Public Health for various diagnostic tests were positive in the SEA test, the significance of which is not ascertained. Different antibodies are responsible for the SEA and the agglutination-lysis reactions.