Purification of Native Dengue-2 Viral Proteins and the Ability of Purified Proteins to Protect Mice

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  • Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, DC

Both the envelope structural protein and the non-structural NS1 protein have been purified from the flavivirus dengue-2 by high-pressure liquid chromatography. These purified proteins maintain their reactivity with monoclonal antibodies. When tested in mice, the envelope protein elicited neutralizing antibodies and partially protected the animals against a lethal viral challenge. The mice responded to the non-structural protein by producing antibodies; however, these antibodies were not neutralizing and the mice were not protected.