Cultivation of Sexually Mature Brugia malayi In Vitro

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  • U.S. Naval Medical Research Unit No. 2, Jakarta Detachment, Jakarta, Indonesia

Sexually mature male and female Brugia malayi were developed from third stage larvae after 60 days in the in vitro culture system described by Franke and others in 1987 (Am J Trop Med Hyg 37: 370–375). Between 75 and 100 days in culture, many worms produced living microfilariae. Each gravid female produced 200–1,500 microfilariae/day.