• 1

    Rosen L., 1981. The use of Toxorhynchites mosquitoes to detect and propagate dengue and other arboviruses. Am. J. Trap. Med. Hyg., 30: 177 183.

    • Search Google Scholar
    • Export Citation
  • 2.

    Rosen L.,, Shroyer D. A., Tesh R. B, Freier J. E., Lien J. C., 1983. Transovarial transmission of dengue viruses by mosquitoes: Aedes albopictus and Aedes aegypti. Am. J. Trap. Med. Hyg., 32: 1108 1119.

    • Search Google Scholar
    • Export Citation
  • 3

    Rosen L.,, 1984. Use of mosquitoes to detect and propagate viruses. 281 292 Maramorosch K., Koprowski H., eds., Methods in Virology VIII Academic Press, Inc., Orlando

    • Search Google Scholar
    • Export Citation
  • 4

    Kuberski T. T., Rosen L., 1977. A simple technique for the detection of dengue antigen in mosquitoes by immunofluorescence. Am. J. Trap. Med. Hyg., 26: 533 537

    • Search Google Scholar
    • Export Citation
  • 5.

    Thompson W. H., 1979. Higher venereal infection and transmission rates with La Crosse virus in Aedes triseriatus engorged before mating. Am. J. Trap. Med. Hyg. 28: 890 896.

    • Search Google Scholar
    • Export Citation
  • 6.

    Tesh R. B., 1981. Vertical transmission of arthropod-borne viruses of vertebrates 122 137 McKelvey J. J., Eldridge Jr., B. F., Maramorosch K.,eds., Vectors of Disease Agents. Praeger Publishers, New York.

    • Search Google Scholar
    • Export Citation
  • 7

    Rosen L, 1987. On the mechanism of vertical transmission of dengue virus in mosquitoes. C. R. Acad. Sci. (Serie III) (Paris), 304: 347 350.

    • Search Google Scholar
    • Export Citation
  • 8

    Aragao H. de B., 1929. On infection of the male “Aedes aegypti” and the possibility of propagation of yellow fever from Stegomyia to Stegomyia without passage through man. Mem. Inst. Oswaldo Cruz, Suppl. 9: 193 195.

    • Search Google Scholar
    • Export Citation
  • 9

    Hindle E., 1930 The transmission of yellow fever. Lancet 2: 835 842.

  • 10

    Simmons J. S., St. John J. H., Holt R. L., Reynolds F. H. K., 1931 The possible transfer of dengue virus from infected to normal mosquitoes during copulation. Am. J. Trop. Med. 11: 199 216.

    • Search Google Scholar
    • Export Citation
  • 11

    Kerr J. A., Hayne T. B., 1932. On the transfer of yellow fever virus from female to male Aedes aegypti. Am. J. Trop. Med., 12: 255 261.

  • 12

    Mattingly P. F., 1946 A technique for feeding adult mosquitoes. Nature, 158: 751.

  • 13

    Cornet M., Robin Y., Heme G., Adam C., Renaudet J., Valade M., Eyraud M., 1979. Une poussée épizootique de Fièvre jaune selvatique au Sénégal oriental. Isolement du virus de lots de Moustiques adultes males et femelles. Méd. Mal. Infectieuses, 9: 63 66.

    • Search Google Scholar
    • Export Citation
  • 14

    Cordellier R., Bouchité B., Roche J. C., Monteny N., Diaco B., Akoliba P., 1983. Circulation selvatique du virus Dengue 2 en 1980, dans Ies savanes sub-soudaniennes de COte d‘Jvoire. Données entomologiques et considerations épidémiologiques. Cah. O.R.S. T.O.M., Sér. Ent. Méd. Parasitol., 21: 165 179.

    • Search Google Scholar
    • Export Citation
  • 15

    Cornet M., Saluzzo J. F., Hervy J.P., Digoutte J. P., Germain M., Chauvancy M. F., Eyraud M., Ferrara L., Heme G., Legros F., 1984. Dengue 2 au Senegal oriental: Une poussee epizootique en milieu selvatique; isolements du virus à partir de moustiques et d‘un singe et considerations épidémiologiques. Cah. O.R.S. T.O.M., Ser. Ent. Med. Parasitol., 22: 313 323.

    • Search Google Scholar
    • Export Citation
  • 16

    Cordellier R., Chippaux A., Monteny N., Hème G., Courtois B., Germain M., Digoutte J-P., 1982. Jsolements du virus Orungo a partir de femelles et de males d‘Aedes selvatiques captures en Cote d‘voire. Cah. O.R.S. T.O.M. , Sér. Ent. Méd. Parasitol., 20: 265 267.

    • Search Google Scholar
    • Export Citation
  • 17

    Thompson W. H., Beaty B. J., 1977. Venereal transmission of La Crosse (California encephalitis) arbovirus in Aedes triseriatus mosquitoes. Science, 196: 530 531.

    • Search Google Scholar
    • Export Citation
  • 18

    Thompson W. H., Beaty B. J., 1978. Venereal transmission of La Crosse virus from male to female Aedes triseriatus. Am. J. Trop. Med. Hyg., 27: 187 196.

    • Search Google Scholar
    • Export Citation
  • 19

    Ovenden J . R., Mahon R. J., 1984. Venereal transmission of Sindbis virus between individuals of Aedes australis (Diptera: Culicidae). J. Med. Entomol., 21: 292 295.

    • Search Google Scholar
    • Export Citation
  • 20

    Nayar J. K., Rosen L., Knight J. W., 1986. Experimental vertical transmission of Saint Louis encephalitis virus by Florida mosquitoes. Am. J. Trop. Med. Hyg., 35: 1296 1301.

    • Search Google Scholar
    • Export Citation
  • 21

    Brun G., Plus N., 1980. The viruses of Drosophila. 625 702 Ashburner M., Novitski E.,eds., Genetics and Biology of Drosophila, Volume 2D. Academic Press, New York.

    • Search Google Scholar
    • Export Citation

 

 

 

 

Sexual Transmission of Dengue Viruses by Aedes Albopictus

View More View Less
  • Arbovirus Program, Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96822

Male Aedes albopictus experimentally infected with dengue virus types 1, 2, 3, or 4 transmitted their infection sexually to female Ae. albopictus. Such transmission was enhanced if the females had taken a bloodmeal 2 to 7 days prior to mating. Male Ae. albopictus also transmitted dengue virus vertically to their F, progeny. Infected progeny were found among those derived from eggs laid ≥73 hr after mating but not among those derived from eggs laid prior to that time. This suggests that virus probably was not transmitted directly to ova but, rather, underwent prior replication in the female genital tract. Female Ae. albopictus experimentally infected with dengue type 1 virus did not transmit their infection sexually to males. This finding supports the hypothesis that male mosquitoes naturally infected with dengue virus acquired their infection vertically.

Save