• 1.

    Tavares C. A. P., de Rossi R., Payares G., Simpson A.J.G., McLaren D.J., Smithers S. R., 1984. A monoclonal antibody against adult Schistosoma mansoni which recognizes a surface antigen on schistosomula. Z. Parasitenk., 70 :189197.

    • Search Google Scholar
    • Export Citation
  • 2.

    Payares G., Kelly C., Smithers S. R., Evans H. W., 1985. Evidence that the 32, 38 and 20 kilodalton surface antigens ofschistosomula and schistosomes are a family of conserved proteins. Mol. Biochem. Parasitol., 17 :115130.

    • Search Google Scholar
    • Export Citation
  • 3.

    Dissous C., Grzych J. M., Capron A., 1982. Schistosoma mansoni surface antigen defined by a rat monoclonal IgG2a. J. Immunol., 129 :22322234.

    • Search Google Scholar
    • Export Citation
  • 4.

    Taylor D. W., Hayunga E. G., Vannier W. E., 1981. Surface antigens of Schistosoma mansoni. Mol. Biochem. Parasitol., 3 :157168.

  • 5.

    Dresden M. H., Sung C. K., Deelder A. M., 1983. A monoclonal antibody from infected mice to Schistosoma mansoni egg proteinase. J. Immunol., 130 :13.

    • Search Google Scholar
    • Export Citation
  • 6.

    Deelder A. M., El-Dosoky I., van Marek E. A. E., Qian Z. L., 1985. Immunofluorescent localization of Schistosoma mansoni circulating cathodic antigen in tissues of infected mice using monoclonal antibody. Z. Parasitenk., 71 :317323.

    • Search Google Scholar
    • Export Citation
  • 7.

    El-Dosoky I., van Marek E. A. E., Deelder A.M., 1984. Presence of Schistosoma mansoni antigens in liver, spleen and kidney of infected mice: A sequential study. Z. Parasitenk., 70 :491497.

    • Search Google Scholar
    • Export Citation
  • 8.

    de Water R., Fransen J. A. M., Deelder A. M., 1986. Ultrastructural localization of the circulating cathodic antigen in the digestive tract of various life-cycle stages of Schistosoma mansoni. Z. Parasitenk., 72 :635646.

    • Search Google Scholar
    • Export Citation
  • 9.

    de Water R., Fransen J. A. M., Deelder A. M., 1986. Ultrastructural localization of the circulating anodic antigen in the digestive tract of Schistosoma mansoni using monoclonal antibodies in an immunogold labeling procedure. Am. J. Trop. Med. Hyg., 35 :549558.

    • Search Google Scholar
    • Export Citation
  • 10.

    Nash T. E., 1974. Localization of the circulating antigen within the gut of Schistosoma mansoni. Am. J. Trop. Med. Hyg., 23 :10851087.

    • Search Google Scholar
    • Export Citation
  • 11.

    Aronstein W. S., Strand M., 1985. A glycoprotein antigen of Schistosoma mansoni expressed on the gynecophoral canal of mature male worms. Am. J. Trop. Med. Hyg., 34 :508512.

    • Search Google Scholar
    • Export Citation
  • 12.

    Qian Z. L., Deelder A. M., 1982. Circulating antigens in Schistosoma infections. Acta Leidensia, 49 :7180.

  • 13.

    Van Marek E. A. E., Jacob W., Deelder A. M., Gigase P. L. J., 1979. Experimental schistosomal glomerulopathy in mice and its relation to porto-systemic collateral circulation: A light and electron microscope study. Ann. Soc. Belge Med. Trop., 59 :3347.

    • Search Google Scholar
    • Export Citation
  • 14.

    Roth J., Bendayan M., Carlemalm E., Villeger W., Garavito M., 1981. Enhancement of structural preservation and immunocytochemical staining in low-temperature embedded pancreatic tissue. J. Histochem. Cytochem., 29 :663671.

    • Search Google Scholar
    • Export Citation
  • 15.

    Deelder A. M., Komelis D., Makbin M., Noordpool H. N., Codfried R. M., Rotmans J. P., Oostburg B. F. J., 1980. Applicability of different antigen preparations in the enzyme-linked immunosorbent assay for schistosomiasis mansoni. Am. J. Trap. Med. Hyg., 29 :401410.

    • Search Google Scholar
    • Export Citation
  • 16.

    Hawkes R., Niday E., Gordon J., 1982. A dot-immunobinding assay for monoclonal and other antibodies. Analyt. Biochem., 119 :142147.

  • 17.

    Towbin H., Staehlin T., Gordon G., 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA, 76 :43504354.

    • Search Google Scholar
    • Export Citation
  • 18.

    Deelder A. M., van Dalen D. P., van Egmond J. G., 1978. Schistosoma mansoni: Microfluorometric determination of circulating anodic antigen and antigen-antibody complexes in infected hamster serum. Exp. Parasitol., 44 :216224.

    • Search Google Scholar
    • Export Citation
  • 19.

    The T. H., Feltkamp T. E. W., 1970. Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology, 18 :865873.

    • Search Google Scholar
    • Export Citation
  • 20.

    The T. H., Feltkamp T. E. W., 1970. Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method. Immunology, 18 :872881.

    • Search Google Scholar
    • Export Citation
  • 21.

    Stirewalt M. A., 1974. Schistosoma mansoni: Cercaria to schistosomule. Pages 15182 in Dawes B., ed., Advances in Parasitology, Volume 14. Academic Press, London.

    • Search Google Scholar
    • Export Citation
  • 22.

    Ebrahimzadeh A., Kraft M., 1969. Ultrastructurelle Untersuchungen zur Anatomie der Cercarien von Schistosoma mansoni. z. Parasitenk., 32 :157175.

    • Search Google Scholar
    • Export Citation
  • 23.

    Meuleman E. A., 1972. Host-parasite interrelationships between the freshwater pulmonate Biomphalaria pfeifferi and the trematode Schistosoma mansoni. Neth. J. Zool., 22 :355427.

    • Search Google Scholar
    • Export Citation
  • 24.

    Senft A. W., Philpott D. E., Pelofsky A. H., 1961. Electron m1croscope observations of the tegument, flame cells, and the gut of Schistosoma mansoni. J. Parasitol., 47 :217229.

    • Search Google Scholar
    • Export Citation
  • 25.

    Williams J. B., 1981. The protonephridial system of Temnocephala novaezaelandiae: Structure of the flame cells and the main vessels. Aust. J. Zool., 29 :131146.

    • Search Google Scholar
    • Export Citation
  • 26.

    Pan C. T., 1980. The fine structure of the miracidium of Schistosoma mansoni. J. Invert. Pathol., 36 :307372.

 

 

 

 

Three Monoclonal Antibodies with Specific Binding Activity to the Excretory System of Schistosoma Mansoni: An Immunoelectron Microscopic Study Using the Gold Labeling Technique

View More View Less
  • 1 Laboratories for Parasitology, Wassenaarseweg 62, NL-2333 AL Leiden
  • 2 *Electron Microscopy, Rijnsburgerweg 10, NL-2333 AA Leiden, University of Leiden, The Netherlands

This study describes three monoclonal antibodies against the excretory system of Schistosoma mansoni. Immunofluorescence revealed antigens forming part of the excretory system of cercariae, adult worms, and miracidia, which were located on the luminal membranes of flame and first tubule cells by immunoelectron microscopy. These antigens are either structural components of the membranes or they derive from excretory fluid and are absorbed during transport and ultrafiltration. Binding specificity of the monoclonal antibodies was tested by immunoelectrophoresis and competitive immunofluorescence; one or two antigens were recognized by each. Reactivity of the antigens after treatment with 7.5% trichloroacetic acid or Rossman's fixative demonstrates at least partial polysaccharide content.

Save