• 1.

    Wahlgren M., Berzins K., Perlmann P., Bjorkman A., 1983. Characterization of the humoral immune response in Plasmodium falciparum malaria. I. Estimation of antibodies to P. falciparum or human erythrocytes by means of micro ELISA. Clin. Exp. Immunol., 53 :127134.

    • Search Google Scholar
    • Export Citation
  • 2.

    Wahlgren M., Bjorkman A., Perlmann H., Berzins K., Perlmann P., 1986. Anti-Plasmodium falciparum antibodies acquired by residents in a holoendemic area of Liberia during development of clinical immunity. Am. J. Trap. Med. Hyg., 35 :2229.

    • Search Google Scholar
    • Export Citation
  • 3.

    Perrin L. H., Dayal R., Reider H., 1981. Characterization of antigens from erythrocyte stages of Plasmodium falciparum reacting with human immune sera. Trans. R. Soc. Trap. Med. Hyg., 75 :163165.

    • Search Google Scholar
    • Export Citation
  • 4.

    Perlmann H., Berzins M., Wahlgren K., Carlsson J., Bjorkman A., Patarroyo M. E., Perlmann P., 1984. Antibodies in malarial sera to parasite antigens in the membrane of erythrocytes infected with early asexual stages of Plasmodium falciparum. J. Exp. Med., 159 :16861704.

    • Search Google Scholar
    • Export Citation
  • 5.

    Jensen J. B., Boland M. T., Akood M., 1982. Induction of crisis forms in cultured Plasmodium faciparum with human immune sera from Sudan. Science, 216 :2301233.

    • Search Google Scholar
    • Export Citation
  • 6.

    Languillon J., 1957. Carte epidemiologique du paludismeau Cameroon. Bull. Soc. Path. Exot., 50 :585600.

  • 7.

    Trager W., Jensen J. B., 1976. Human malaria parasites in continuous culture. Science, 193 :673675.

  • 8.

    Voller A., Bidell D., Huldt G., Engvall E., 1974. A microplate method of enzyme-linked immunosorbent assay and its application to malaria. Bull. W.H.O., 51 :209211.

    • Search Google Scholar
    • Export Citation
  • 9.

    Dodge C. M., Mitchell C. , Hanahan D., 1963. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch. Biochem. Biophys., 100 :119130.

    • Search Google Scholar
    • Export Citation
  • 10.

    Lambros C., Vanderberg J. P., 1979. Synchronization of Plasmodium falciparum erythrocytic stages in culture. J. Parasitol., 65 :418420.

  • 11.

    Kessler S. W., 1975. Cell membrane antigen isolation with the staphylococcal protein A-antibody adsorbent. J. Immunol., 117 :14821490.

    • Search Google Scholar
    • Export Citation
  • 12.

    Laemmli U. K., Favre M., 1973. Maturation of the head of bacteriophage T4: DNA packaging events. J. Molec. Biol., 80 :575599.

  • 13.

    Chamberlain J. P., 1979. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal. Biochem., 98 :132135.

    • Search Google Scholar
    • Export Citation
  • 14.

    Lyon J. A., Haynes J. D., Diggs C. L., Chulay J. D., Pratt-Rossiter J. M., 1986. Plasmodium falciparum antigens synthesized by schizonts and stabilized at the merozoite surface by antibodies when schizonts mature in the presence of growth inhibitory immune serum. J. Immunol., 136 :22522258.

    • Search Google Scholar
    • Export Citation
  • 15.

    Brown G. V., Stace J. D., Anders R. F., 1983. Specificities of antibodies boosted by acute Plasmodium falciparum infection in man. Am. J. Trap. Med. Hyg., 32 :12211228.

    • Search Google Scholar
    • Export Citation
  • 16.

    Brown G. V., Anders R. F., Mitchell G. F., Heywood P. F., 1982. Target antigens of purified human immunoglobulins which inhibit growth of Plasmodium falciparum in vitro. Nature, 297 :591593.

    • Search Google Scholar
    • Export Citation

 

 

 

 

Shared Polypeptides from Various Strains of Plasmodium Falciparum Recognized by Cameroon Sera

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  • Section of Parasitology, Department of Microbiology, Immunology and Parasitology, Louisiana State University Medical Center, New Orleans, Louisiana 70112

Sera collected from 176 individuals residing in three malaria-endemic regions of Cameroon, West Africa, were tested against Plasmodium falciparum isolates from different areas of the world by enzyme-linked immunosorbent assay (ELISA), growth inhibition studies, and immunoprecipitation assays. A wide range of parasite proteins with apparent molecular weights of 14 to 250 kilodaltons (Kd) were immunoprecipitated by the sera. Of these, two polypeptides of 41 and 96 Kd could be associated with parasite growth inhibition in vitro.

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