A New Liquid Medium without Blood and Serum for Culture of Hemoflagellates

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  • Gonçalo Moniz Research Center, FIOCRUZ, Federal University of Bahia, Salvador, Brazil

A liquid medium without blood or serum was developed for cultivation of hemoflagellates. To a basic LIT medium containing liver infusion broth and tryptose, a mixture of RPMI 1640 and Medium 199 was added. This combination permitted high parasite yields useful for biochemical and immunological studies.