A Schistosoma Mansoni Surface Glycoprotein Cross-Reactive with a T1 Antigen of Fasciola Hepatica

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  • Department of Pharmacology and Experimental Therapeutics, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205

An antigen recognized by monoclonal antibody 306B3/5 and present on the surface of S. mansoni was expressed by F. hepatica in the pattern typical of T1 antigens. A single polypeptide of molecular weight (Mr) 160,000 was precipitated from metabolically labeled concanavalin A-binding F. hepatica glycoproteins, whereas multiple polypeptides ranging from Mr > 200,000 to Mr 45,000 were precipitated from metabolically labeled glycoproteins of male S. mansoni. The polypeptides of both species were also precipitated by sera of infected hosts, and may account for some of the serological cross-reactivity between S. mansoni and F. hepatica in immunodiagnostic assays. These antigens may also represent potentially immunoprophylactic reagents.