Growth and Development of Two Trypanosoma Cruzi Clones in the Arthropod Dipetalogaster Maximus

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  • Laboratorio de Fisiologia de Insetos, Departamento de Fisiologia, Universidade Federal Fluminense, Brasil and Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 24210 Niterio RJ, U.S.A.

Trypomastigotes of single-cell-isolate Trypanosoma cruzi clones WA-250/1 and Esmereldo/2 were used to infect third-instar Dipetalogaster maximus. The level of infection was directly proportional to the concentration of trypomastigotes present in the blood meal. The minimum infectious dose for D. maximus was calculated as 5 organisms/ml. Clone WA-250/1 underwent epimastigote-to-metacylic trypomastigote transformation over the 30-day course of the experiments; clone Esmereldo/2 did not undergo transformation to metacylic trypomastigotes. These data extend our knowledge of the biological diversity of T. cruzi stocks.

Author Notes

Supported in part by a fellowship from the Pan American Health Organization.

Address requests for reprints to Dr. James A. Dvorak, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20205.