Surface Coagglutination with Formalinized, Stained Protein a Staphylococci in the Immunologic Study of Three Pathogenic Amebae

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  • Lilly Laboratory for Clinical Research, Bureau of Laboratories, Indiana State Board of Health, 1001 West Tenth Street, Indianapolis, Indiana 46202

The indirect immunofluorescence and, to a lesser extent, the immunoperoxidase methods have become the principal means of conducting immunological studies upon amebae. These procedures require the conjugation of fluorescein or enzyme with antiglobulin reagents and fixation of the amebae. The present report describes a somewhat similar methodology wherein formalinized, stained protein A staphylococci combined with specific antibody have been found to coagglutinate upon a portion of the amebic surface of living trophozoites in a fashion similar to direct immunofluorescence. The prepared staphylococci without attached antibody have also been found to coagglutinate in an identical fashion after the specific antibody has been deposited upon the amebic surface. This is comparable to indirect immunofluorescence reaction. A comparison of this procedure with the immunofluorescence method of identification and with measurement of titer of serum antibody is presented, which indicates that the new technique compares favorably with the immunofluorescence results.