By H. J. Bensted, W. Bulloch, L. Dudgeon, A. G. Gardner, E. D. W. Greig, D. Harvey, W. F. Harvey, T. J. Mackie, R. A. O'Brien, H. M. Perry, H. Scutze, P. Bruce White, W. J. Wilson. London, 1929. His Majesty's Stationery Office. Pp. 1–482
by A. Trevor Willis, M.D., B.S. (Melb.), Ph.D. (Leeds), M.C.Path., M.C.P.A., Reader in Microbiology, Monash University, formerly Lecturer in Bacteriology, University of Leeds. xiv + 234 pages, illustrated, second edition. Butterworth Inc., Washington. 1965. $8.50
Antigens in extracts of two strains of Entamoeba histolytica (HT-31 and HK-9) were studied by two-dimensional immunoelectrophoresis (2D-IEP) and 32 and 20 different antigenic components were detected, respectively. Cross-reactions between heterologous systems showed a large number of shared antigens between the two strains. Extracts were fractionated by Sephadex G-200 chromatography. Of the fractions obtained, fraction II of both strains was found to have the most reactive antigenic components. Precipitin patterns of fraction II from HT-31 and HK-9 with sera from patients showed similar 2D-IEP profiles and suggested that in this fraction antigens common to both strains exist which elicit antibody in individuals with E. histolytica infections.