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Growth of Babesia Bovis in Bovine Erythrocyte Cultures

Elton E. Erp Department of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Department de Hemoprotozoarios, Instituto Nacional de Investigaciones Pecuarias, Urbana, Illinois 61801, Mexico

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Sally M. Gravely Department of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Department de Hemoprotozoarios, Instituto Nacional de Investigaciones Pecuarias, Urbana, Illinois 61801, Mexico

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Ronald D. Smith Department of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Department de Hemoprotozoarios, Instituto Nacional de Investigaciones Pecuarias, Urbana, Illinois 61801, Mexico

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Miodrag Ristic Department of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Department de Hemoprotozoarios, Instituto Nacional de Investigaciones Pecuarias, Urbana, Illinois 61801, Mexico

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B. Miguel Osorno Department of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Department de Hemoprotozoarios, Instituto Nacional de Investigaciones Pecuarias, Urbana, Illinois 61801, Mexico

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C. A. Carson Department of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Department de Hemoprotozoarios, Instituto Nacional de Investigaciones Pecuarias, Urbana, Illinois 61801, Mexico

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DOI:
https://doi.org/10.4269/ajtmh.1978.27.1061
Volume/Issue:
Volume 27: Issue 5
Page(s):
1061–1064
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Babesia bovis was cultured in a suspension of bovine erythrocytes incubated at 37°C in Medium 199 with 50% bovine serum. The cells in culture were kept in suspension by slow stirring in spinner flasks and the medium was replaced at 24-hour intervals. Persistent multiplication of the parasite in a short series of subcultures suggests the feasibility of this approach for continuous culture.

Author Notes

Copyright:
Copyright © 1978 by The American Society of Tropical Medicine and Hygiene 1978
Accepted:
10 Jun 1978
|
Published Online:
Sep 1978
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Volume 27: Issue 5
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Full Text Views 13 5 0
PDF Downloads 17 8 0
 
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