Infection and Transmission of Eastern Equine Encephalomyelitis Virus with Colonized Culiseta Melanura (Coquillett)

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  • Yale Arbovirus Research Unit and SEction of Medical Entomology, Department of Epidemiology and Public Health, Yale University, School of Medicine, 60 College Street, New Haven, Connecticut 06510

Colonized Culiseta melanura were infected with eastern equine encephalitis (EEE) virus by feeding on viremic 1-day-old chicks. The approximate minimal mosquito infective dose was 104 baby mouse LD50/0.02 ml of blood, the approximate 50% dose was 105, and the approximate 90% dose was 106 LD50/0.02 ml. Over 85% of infected mosquitoes transmitted EEE virus to baby chicks after a 2-week extrinsic incubation.

Author Notes

Present address: New York State Department of Health, 1475 Winton Road North, Rochester, New York 14609.