Immunofluorescent staining of exoerythrocytic schizonts of simian plasmodia in fixed tissue

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  • 1 Primate Malaria Unit, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, P. O. Box 80190, Chamblee, Georgia 30341

Exoerythrocytic schizonts of three simian malarias were readily identified by indirect immunofluorescence in liver biopsy material fixed in Carnoy's solution and embedded in paraffin up to 9 years previously. Freshly embedded and stored materials showed a striking similarity both in intensity of specific fluorescence and in the degree of detail revealed. Parasites of two different species were stained to an essentially equivalent extent through a hyperimmune serum prepared against the blood stages of a single, defined strain of one of the two.

Author Notes

Present address: Department of Medicine, USPHS Marine Hospital, 15th Avenue at Lake Street, San Francisco, California 94118.