Influence of Vitamin E on Reproduction of Biomphalaria Glabrata Under Axenic Conditions

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  • Departamento de Bioquímica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil


A semisynthetic medium that promotes growth of Biomphalaria glabrata under axenic conditions is described. This medium, when enriched with vitamin E, promotes reproduction of the mollusk. The snail was reared over three consecutive generations under germ-free conditions. The sterilization temperature of the medium is critical: when sterilized at higher temperatures than 110°C, the medium does not promote growth.