Susceptibility of the Baby-Hamster Kidney-Cell Line (BHK-21) to Infection with Arboviruses

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  • Yale Arbovirus Research Unit, Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut

Summary

Of 129 different arboviruses (134 strains) inoculated into cultures of the baby-hamster kidney-cell line BHK-21 (clone 13), all but five viruses produced cytopathic effect. The agents tested comprised members of 18 serogroups and a large number of ungrouped arboviruses. Comparative titrations of 10 viruses indicated that BHK-21 tissue culture, although slightly less sensitive than infant mice, could be of value as a routine assay system. Results were satisfactory in a limited series of BHK-21 tissue-culture neutralization tests.

A total of 31 arboviruses, 10 in the larger serogroups and 21 mostly ungrouped, was tested in tissue culture for hemadsorption of guinea-pig erythrocytes. Results were negative except for one ungrouped virus, both strains of which were shown to be a myxovirus. The applicability of the hemadsorption test as a screening method in field laboratories is discussed.

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