INTRODUCTION
Increased coverage with effective malaria control interventions has led to a decrease in the transmission of Plasmodium parasites and a notable reduction in the malaria burden across large parts of the malaria-endemic world.1,2 However, increasing subnational heterogeneity in malaria transmission as a consequence of varying uptake and the effectiveness of control measures requires more detailed and granular data for targeting and tailoring intervention packages. As countries scale up their malaria control efforts and aim at eventually achieving elimination, transforming malaria surveillance into a core intervention becomes increasingly important.3 In practice, surveillance data need to play an increasing role in programmatic decision-making and day-to-day response action.
Several initiatives are underway to improve the collection and reporting of routine surveillance data, most notably the large-scale rollout of standardized, country-owned, and locally customizable systems such as DHIS 2 (formerly District Health Information Software).4 Extensions to DHIS2 and other locally developed systems increasingly allow programs to move from aggregate reporting of clinical cases to individual case–based reporting that forms the basis for case investigation and classification, a requirement for eventual certification of elimination.5
To date, efforts toward consolidating and scaling up routine surveillance for the purpose of programmatic decision-making have focused mainly on malariological indicators that are based on well-established diagnostic tools such as microscopy, a rapid diagnostic test (RDT), or morphological identification of mosquitoes.6–8 Although crucial for monitoring malaria transmission and burden, these data capture only part of the complex malaria transmission dynamics in a given geographical region.9 Furthermore, not all of these indicators can be collected in all settings.
As parasite and mosquito genetic pools are continuously shaped by control strategies, genomic data provide a complementary tool to understand underlying transmission in response to the applied interventions.10 Molecular diagnostic tools can provide a more accurate picture of ongoing transmission,11 and they may hence have the potential to improve malaria surveillance efforts. However, to date, molecular and genomic data have been rarely used in routine surveillance12,13 and are often conducted by research institutions that lack programmatic perspective and decision-making power. Their use is limited, for example, by a lack of adequate infrastructure and well-trained staff in many malaria-endemic countries, especially at the National Malaria Control Program (NMCP) level. Moreover, for some molecular techniques, there is a lack of sufficient evidence and hands-on guidance for their programmatic implementation and application.14
In this review paper, we present current use cases of molecular techniques to guide malaria strategies and explore future use cases of molecular surveillance to inform programmatic decisions for malaria control and elimination efforts.
CURRENT USE CASES OF MOLECULAR TECHNIQUES TO INFORM MALARIA PROGRAMMATIC DECISIONS
There are several use cases for malaria molecular monitoring, but only four of them are currently recommended for routine surveillance, including the distinction between recrudescence and new infections in therapeutic efficacy studies (TESs) for Plasmodium falciparum15 and monitoring of insecticide resistance.16 Although guidelines also exist for monitoring P. falciparum histidine-rich protein 2 and 3 (Pfhrp2/3) genes deletion17 and molecular markers of resistance,18,19 they are rarely systematically performed as part of routine surveillance.
In the following section, we provide an overview of the current and prospective use cases of molecular tools for guiding programmatic decisions, also summarized in Table 1.
Summary of current and prospective use cases of molecular tools for guiding programmatic decisions
Use Case | Description (molecular techniques) | Strengths and Weaknesses |
---|---|---|
Current use cases | ||
Therapeutic efficacy studies | Use highly polymorphic markers to differentiate recrudescence from new infection (nested PCR followed by gel or capillary electrophoresis, PCR-RFLP, NGS) | + Drug efficacy estimates comparable between areas of different transmission intensity − Follow-up of patients is required − PCR limitation in detecting minority clones − Reproducibility between laboratories |
Pfhrp2/Pfhrp3 deletion typing (diagnostic resistance monitoring) | Uses nested PCR, qPCR, or digital PCR, Sanger sequencing, NGS | + Yields conclusive data on deletion status, as false-negative Pfhrp2-based RDTs can have other causes − Nondetection of deleted parasites in polyclonal infections |
Drug resistance monitoring | Uses established genetic markers to assess the frequency of genotypes associated with drug resistance (nested PCR-RFLP, qPCR, Sanger sequencing, NGS) | + Characterizes resistance emergence and spread + No follow-up of patient needed; can be used on archived blood samples − Does not provide a direct estimate of treatment efficacy |
Insecticide resistance monitoring | Uses established genetic markers to assess the frequency of genotypes associated with insecticide resistance (nested PCR, PCR-RFLP, qPCR, Sanger sequencing, NGS) | + Characterizes resistance emergence and spread + Affordable and scalable compared with phenotypic studies − Cannot provide a direct relationship with insecticide efficacy |
Prospective use cases | ||
Sero-surveillance | Uses established antibodies to measure changes in transmission intensity (ELISA, bead-based assays, protein microarrays) | + Allows for a better understanding of transmission heterogeneity in low-transmission settings − Acquired immunity may be a major confounder |
Detecting the sub-patent and asymptomatic reservoir | Uses highly sensitive diagnostic methods to screen for low-density, sub-patent infections (nested PCR and gel electrophoresis, LAMP, qPCR) | + Provides an accurate view of the transmission reservoir and where to target interventions + Increasingly available in reference laboratories in endemic countries − Currently not scalable for point of care − Additional benefit over other tools (case numbers, prevalence by mRDT) unclear − Sensitivity depending on the target gene (18 s, varATS, etc.) and the matrix used for sample collection (whole blood vs. dried blood spot) |
Genomic measures as surrogate markers for transmission changes | Use nPCR/gel-based genotyping, PCR–capillary electrophoresis, qPCR (HRM), NGS | + May be more scalable and affordable than other measures of transmission intensity, especially in areas with low malaria transmission − Links between transmission intensity and parasite diversity/multiplicity are complex and not well understood |
Characterizing transmission chains | Identifies hot spots of transmission and sources of importation; tracks cases in the event of an outbreak (NPCR/gel-based genotyping, PCR–capillary electrophoresis, qPCR [HRM], NGS) | + May allow for new strategies to prevent importation/break transmission chains − Added value compared with travel history reporting by patients not clear |
+ = strengths; − = weaknesses; ELISA = enzyme-linked immunosorbent assay; HRM = high-resolution melting; LAMP = loop-mediated isothermal amplification; mRDT = malaria rapid diagnostic testing; NGS = next generation sequencing; nPCR = nested polymerase chain reaction; PCR = polymerase chain reaction; PCR-RFLP = polymerase chain reaction–restriction fragment length polymorphism; qPCR = quantitative polymerase chain reaction.
Therapeutic efficacy studies.
Polymerase chain reaction (PCR) correction (i.e., comparing the genotype of parasites collected from a patient at enrollment with the genotype of parasites found in the same patient during follow-up) allows distinguishing a new infection (newly acquired genotypes) from a recrudescence (same genotype[s]) in P. falciparum infections. Highly diverse–size polymorphic markers, such as P. falciparum merozoite surface protein 1 and 2 (Pfmsp1 and Pfmsp2) and microsatellites, are used to differentiate recrudescence from new infection by comparing P. falciparum genotypes in pretreatment samples and any posttreatment sample with parasites detectable by microscopy or PCR.20,21 The sensitivity of the different markers in detecting minority clones in polyclonal infections is the main limitation of the techniques used to differentiate recrudescence and new infection.22,23 The recommended WHO protocol for molecular monitoring during clinical trials was recently amended to consider this limitation. Accordingly, the glutamate-rich protein gene (Pfglurp) marker was replaced with microsatellite markers owing to the limitation of Pfglurp in detecting minority clones in polyclonal infections.21 Nevertheless, sampling at a single time point can also result in missed clones because of parasite sequestration and fluctuation in parasite densities.24,25 Furthermore, different data analysis algorithms have been proposed,26–29 and a consensus still needs to emerge about how to validate them.
Polymerase chain reaction correction is still often performed using PCR and agarose gel–based assays. These assays have lower discriminatory power in distinguishing recrudescence from new infection and provide inaccurate estimates of PCR-corrected drug efficacy. There is a need to implement techniques with improved discriminatory power based on capillary electrophoresis or amplicon deep sequencing to precisely estimate amplicon fragment sizes and precisely call haplotypes, respectively; however, many challenges are hampering their wide adoption. Although amplicon deep sequencing (i.e., Illumina sequencing of highly diverse amplicons of a few hundred base pairs) provides higher sensitivity30 and results in higher detectability of minority clones,31 no guidelines currently exist for assay validation, minimum criteria of assay acceptance, and data reporting. This makes it challenging to compare data over time or between different laboratories. Moreover, the costs associated with implementation of this technology, challenges in reagents procurement, training, and limited capacity in data analysis are still major obstacles for implementation in malaria-endemic countries.
The current system based on TESs, conducted in a few sentinel sites, has many limitations. First, because of logistical and economic constraints, TESs are conducted at best every 2 years, although many countries still fail to reach that goal. Second, in high-transmission settings, delayed parasite clearance, which is the hallmark of artemisinin partial resistance, is confounded by a high level of acquired immunity, making it a poor indicator of the emergence of artemisinin partial resistance in these settings.32 Third, TESs are challenging to conduct in low-transmission settings owing to the low number of malaria cases. Additional molecular data, such as the prevalence or frequency of drug resistance markers, could offer a complementary or alternative approach to monitor antimalarial drug resistance, inform the best locations to conduct TES, and confirm resistance.33
Markers of drug resistance.
The molecular basis of resistance to many antimalarials is well understood; thus, typing of respective loci can provide information on the prevalence or frequency of genotypes associated with drug resistance. These results are not confounded by patient immunity and may help to detect early emergence of drug resistance.34 For artemisinin partial resistance, mutations in the PfKelch13 gene have been associated with delayed parasite clearance after treatment with artemisinin combination therapies (ACTs)35,36 or artemisinin monotherapy.37,38 Detection of kelch13 mutations has proven highly effective in monitoring the spread of resistance across the Greater Mekong subregion.39,40 In contrast to TESs, which require follow-up of patients at multiple time points, monitoring resistance markers can be conducted on any blood sample collected from clinical patients or asymptomatic carriers. It is an affordable and highly scalable strategy. Nevertheless, well-equipped laboratories with well-trained staff are required to conduct the analyses using the most appropriate techniques.41
Not all infections with a drug-resistant parasite will result in treatment failure, as resistant infections may be cleared in semi-immune individuals. As a result, there is not always a direct relationship between molecular markers of resistance and treatment failure.42 However, increasing prevalence or frequency of validated molecular markers has often been associated with increasing treatment failure,43–46 and large pooled analyses have confirmed the selection of specific resistance markers after treatment with most currently used ACTs (i.e., artemether-lumefantrine and artesunate-amodiaquine).47,48
Most current drug resistance–marker surveillance activities focus on a few geographical sites and are conducted irregularly. Repeated studies are needed to monitor molecular markers and their dynamics over time and space. To better guide policymakers in antimalarial treatment policy change, the development of tools to dynamically map the prevalence of resistance markers and predict treatment outcomes on a population level is needed.49,50
Diagnostic resistance.
Malaria case management is based on prompt diagnosis and treatment of confirmed cases. This strategy relies on easy access to malaria diagnosis at each level of the health system. Over the last 10 years, malaria RDTs (mRDTs) have become the mainstay for malaria diagnosis in malaria-endemic countries, especially in sub-Saharan Africa.2,51 However, this strategy is threatened by the emergence of parasites lacking the Pfhrp2 and 3 genes, the target of the most sensitive available RDTs for P. falciparum.52–54
Plasmodium falciparum parasites lacking the Pfhrp2/3 genes were initially reported from Peru55 and since then in many other malaria-endemic countries, especially in the Horn of Africa.56,57 Two countries in sub-Saharan Africa (i.e., Eritrea and Ethiopia) have reported a very high prevalence of Pfhrp2/3 genes deletion, leading Eritrea to change its national malaria diagnosis policy from histidine-rich protein 2 (HRP2)-based RDTs to plasmodium lactate dehydrogenase (pLDH)-based RDTs.58–60
The WHO has developed guidance for monitoring Pfhrp2/3 deletions,17,61 and multiple molecular assays for deletion typing are available.62–65 There is a need to develop laboratory capacity for applying these assays and for the associated high throughput molecular analyses in malaria-endemic countries. Such a laboratory is currently being established in Ethiopia (C. K., personal communication).
Insecticide resistance.
Susceptibility or intensity bioassays, including the CDC’s bottle bioassays, the World Health Organization Pesticide Evaluation Scheme cone bioassay, and the WHO bottle bioassay, remain the gold standard for monitoring vector resistance to insecticides,66 but logistical challenges and high intra-assay variability impede surveillance.67 Molecular assays are currently performed only to confirm the underlying mechanism of resistance after detection of phenotypic resistance with bioassays.68 However, despite previous studies showing functional links between molecular markers and insecticide resistance,69 bioassay outcomes do not always correlate with molecular data.70 Although the genotype–phenotype relationship for insecticide resistance is not fully understood, bioassay data may be additionally impacted by other factors, such as environmental factors or metabolic resistance,67,71 impeding their association with molecular data. It is currently recommended that molecular species characterization be conducted after the bioassays to confirm morphological characterization and to identify species groups that cannot be differentiated morphologically.16 Logistical challenges, high intra-assay variability, and data interpretation and standardization challenges associated with bioassays impede insecticide resistance surveillance.72,73
Several molecular markers for resistance to each major insecticide class have been established. One such example is knockdown resistance (kdr) mutations conferring resistance to dichlorodiphenyltrichloroethane and pyrethroids.74 Molecular surveillance can be used to monitor temporal and spatial trends of vector resistance, although this strategy is not yet commonly used.75,76 Despite implementation of monitoring of markers such as kdr mutations in several locations,77–80 little is known about the correlation between the prevalence of molecular markers and insecticide efficacy in the field, and more data are needed to fill this gap.81 Understanding of the current status of insecticide resistance and the drivers of vector control efficacy can be addressed through molecular surveillance by simultaneously collecting bioassay data, field efficacy of insecticide-based interventions, and the prevalence of molecular markers.
PROSPECTIVE USE CASES FOR MOLECULAR TOOLS
Detection of the malaria transmission reservoir.
Currently, the commonly used mRDTs are missing a significant number of infections both in low- and high-transmission settings, especially low-density infections and parasites with deletion of Pfhrp2/3 genes.82–84 Polymerase chain reaction (or quantitative PCR [qPCR]) is substantially more sensitive than microscopy or an RDT, reaching a limit of detection of < 1 parasite/µL of blood. Polymerase chain reaction screening can be applied to determine the proportion of infections missed by routine diagnosis and to screen for low-density, sub-patent individuals to identify hidden parasite reservoirs. Numerous studies have shown a large sub-patent reservoir under all transmission intensities.85,86 Although PCR was often only available in a relatively small number of research laboratories in the past, slowing down time to results, it is now widely available in endemic countries. Furthermore, portable devices allow screening on-site and thus a much faster turnaround time.87–89
Despite its wide use in research, PCR-based molecular screening for infections has led directly to interventions or policy change in only a few documented cases. In Myanmar, qPCR is usually applied to stratify villages into low or high prevalence, with the latter receiving mass drug administration (MDA).90 However, although estimates by qPCR are highly accurate, the cost is high, and screening by an RDT combined with a lower detection prevalence threshold for deploying MDA might be more cost-effective. Another example is the Chinese 1-3-7 approach in which confirmation of infection by qPCR is established before case investigation is conducted.91 According to this approach, cases are reported within 1 day and investigated within 3 days, and reactive investigation of the focus is conducted within 7 days. Polymerase chain reaction confirmation is warranted owing to the relatively small number of cases and extensive investigation of each case. On the opposite, during the elimination phase in Sri Lanka, microscopy was the main method used for malaria diagnosis, and RDTs results were always confirmed by microscopy. However, this required the establishment of an extensive microscopy quality assurance program, including proficiency testing and continuous training of the microscopists.92
Molecular tools such as qPCR might become more widely integrated into routine surveillance, especially in low-transmission areas or in settings advancing toward elimination.
Polymerase chain reaction has also been established in reference laboratories in other countries that are in the elimination phase (e.g., Afghanistan).93 Furthermore, it has been frequently used in research studies to assess the impact of interventions94 and for monitoring sub-patent infections.95,96 To increase throughput and reduce costs, multiple samples can be pooled for qPCR analysis. If a pool is positive, samples can be tested individually.97,98 It remains to be seen whether the extensive infrastructure for molecular testing built up in many countries in response to COVID-19 may in the future be leveraged for the molecular surveillance of other pathogens, including malaria.99
Multiplicity of infection (MOI) as a proxy for transmission intensity.
Different indices of genetic diversity are being evaluated as surrogate markers for transmission intensity. The MOI (or complexity of infection) is among the most frequently reported genetic measures. It can be easily determined using any of multiple genotyping techniques. Some small-scale, geographic-specific studies have found a strong correlation between MOI and other measures of transmission intensity (e.g., clinical incidence or prevalence), in particular over extended periods of time. Nevertheless, a systematic review of the relationship between MOI and prevalence did not find a clear pattern.100 Likewise, the relationship between other genetic diversity metrics and transmission intensity is poorly characterized. It appears that MOI starts to decline only once transmission has been reduced to very low levels (reviewed in Koepfli and Mueller101 and Noviyanti et al.102). Owing to these complexities, genomic indicators are not routinely used by control programs.
Parasite relatedness, detecting importation, and characterizing transmission chains.
More sophisticated genomic analysis, including genotyping a higher number of markers or whole genome sequencing (WGS), and advanced tools for data analysis can provide insights into parasite movements across space and time.103 Once transmission is very low, it becomes crucial to differentiate between imported and locally transmitted cases. Parasite genotyping might be able to do that, but it will rely on a good understanding of the genomic composition of the local parasite population as well as populations that might serve as sources for importation.101,104 Baseline and subsequent cross-sectional surveys to generate WGS data in low- to very low–transmission settings would provide a better understanding in this regard,10 as they can be used in mathematical models to elucidate the structure of parasite populations and transmission networks to target and tailor interventions accordingly.105–107 Almost all existing studies on genetic diversity, MOI, and transmission networks were conducted at a time when changes in transmission were already evident by changing clinical case numbers. Likewise, self-reported travel histories used to be sufficient to classify a case as imported versus locally transmitted. However, self-reported travels may not be able to reliably disentangle local transmission chains, as transmission in areas approaching elimination is very complex and may be due mainly to imported cases, despite a large local sub-patent parasite reservoir.108 Moreover, compared with travel history and mobile phone data, genomic data can provide better estimates of parasite movements and connectivity over longer distances,109 paving the way to regional activities for malaria control and elimination.
Sero-surveillance.
Serological markers to monitor recent or past exposure to parasites or vectors hold great promise for monitoring control efforts and may serve to identify at-risk populations or hot spots, where preventive measures should be implemented. Applications have been reviewed in detail elsewhere110,111 and are summarized briefly here.
In particular, when transmission becomes very low, clinical cases or asymptomatic infections are too few to provide meaningful information in heterogeneity of transmission or recent changes in response to control. Measuring antibody titers as markers of past exposure to parasites can provide higher power to assess differences. For example, in western Kenya, seroprevalence across multiple clusters was around 5-fold higher than prevalence by PCR and 10-fold higher than prevalence by RDT.112 Seroprevalence data thus allowed for a better understanding of heterogeneity in transmission than parasite prevalence data alone.
When very few parasites are present in a population, sero-surveillance can be applied to quantify transmission in the population. With use of different antibodies with different half-lives, it may be possible to assess cumulative and recent exposures. Recent studies have shown that antibodies such as Ama-1 and msp1 may be associated with cumulative exposure, whereas Etramp and Glurp are associated with recent or current infection.113,114 Even in the absence of current or recent infections, individuals at higher exposure to vectors are expected to be at risk of getting infected. Bites of infected and uninfected mosquitoes result in a relatively short-lived immune response. The gSG6-P1 peptide was identified as a marker to Anopheles bites115 and was used to stratify exposure in multiple settings.116,117 Antibodies for this assay were successfully extracted from archived RDTs collected by the National Malaria Elimination Program in Bangladesh,118 thus minimizing the efforts for field collection of specimens. Highly multiplexed assays have also been developed allowing assessment of hundreds or thousands of antibodies at the same time and helping to identify new markers for parasite or mosquito exposure.119,120
The main limitation of all these molecular techniques is the requirement of human blood samples. Because it may not always be possible to collect the required samples for molecular analyses, an alternative would be to use mosquitoes. Indeed, DNA extracted from mosquitoes may be suitable not only for insecticide resistance monitoring but also for parasite drug resistance monitoring or Pfhrp2/3 deletion typing. Mosquitoes can thus also be used as a source of parasite DNA, and previous studies have shown that this could be a fast and cost-effective strategy,121,122 alleviating the need to collect blood samples from humans. However, this strategy will be likely applied in high-transmission settings, where routine entomological surveillance is regularly conducted and mosquito infection rates are assessed.
THE ROLE OF MATHEMATICAL MODELING
Data derived from routine epidemiological surveillance and representative surveys have been previously successfully used to guide policy decisions in numerous use cases.123–125 For this purpose, mathematical modeling and statistical analyses have proven instrumental in extracting quantitative evidence from collected surveillance data and informing programmatic decisions. Frameworks using epidemiological data for statistical analyses and to parameterize mathematical models of malaria transmission have been set up and used by countries to guide their national malaria strategic plans.126 A nonexhaustive list of these applications includes identification and stratification of malaria risk,127–129 optimization of interventions for national strategic planning,130 and support for funding requests to the Global Fund.131,132 Furthermore, routine surveillance data and quantitative risk assessment combined with statistical and model-based analyses have constituted a key component of the National Malaria Elimination Program in China.133
Despite the existing data and modeling frameworks for decision-making and their uptake by certain NMCPs, only a few small-scale applications have included molecular surveillance data. Of note, in this context, mathematical models have been previously used to link parasite diversity to changes in transmission intensity,134,135 map the drivers and spread of Pfhrp2 deletions,136 and drug resistance,137,138 identify outbreaks,139 and characterize flows of infections.107 However, despite their usefulness,104 these applications have been mostly restricted to a specific geographical setting rather than applied countrywide and were performed mostly for research purposes rather than with an actionable programmatic implication for malaria control and elimination strategies. The development of analytical methods and extension of current mathematical models to include molecular surveillance data could bring valuable insights and complement the current efforts of national malaria control and elimination programs.
Mathematical modeling can be used to leverage the available molecular data collected so far. In the context of TESs, the current algorithm recommended by the WHO to distinguish recrudescence from new infections has some limitations, as it does not consider uncertainty associated with the markers and the technique used for genotyping.21,140 More robust algorithms providing a certain degree of uncertainty in genotyping results are needed to help policymakers make the most appropriate decisions. Several recent algorithms have been developed for distinguishing recrudescence from new infections based on dynamical modeling and Bayesian statistics.26–28 A systematic benchmarking of these methods on multiple existing molecular datasets would allow establishing a standardized algorithm that could be readily applicable in the countries where TESs are conducted.
Having been previously used for estimating the temporal and geographic distribution of malaria burden,129,141,142 geospatial methods can be used to construct monitoring tools for the spread of Pfhrp2 deletions and drug resistance markers.49,50 These tools could be incorporated in existing monitoring dashboards (e.g., DHIS2) that are already in place, but are currently based mostly on epidemiological indicators. However, although informative, the spatiotemporal distribution of molecular markers is challenging to interpret for programmatic purposes. For instance, the relationship between molecular markers of drug or insecticide resistance and drug efficacy or insecticide resistance, respectively, is yet unclear as many confounding factors play an important role.143–145 Previous modeling analyses have explored the potential contribution of biological, epidemiological, and treatment factors of the establishment and spread of drug resistance,137,146 as well as optimal treatment schemes to prevent drug resistance establishment and spread.147,148 Nevertheless, there remains a need to develop complementary tools to quantitatively understand the relationship between the prevalence of molecular markers of drug resistance and treatment efficacy by leveraging the collected molecular marker data. By analysis of existing datasets, quantitative methods can be developed to further understand these relationships. Developing models that can estimate these relationships on a population level and over time could provide a valuable tool to estimate the efficacy life span of deployed antimalarial drugs or insecticides and help policymakers plan well ahead of potential changes in drug or insecticide policy.
INCORPORATING MOLECULAR SURVEILLANCE DATA INTO PROGRAMMATIC AND POLICY DECISION-MAKING
Combining molecular surveillance data with existing routine epidemiological indicators to guide NMCPs comes with its own challenges. First, collection of molecular data is not yet standardized, and countries need capacity for sample collection, storage, processing, and analysis. Although sample size recommendations are available for some use cases (e.g., for Pfhrp2/3 deletion genotyping), no guidance exists on the number of samples to be screened to determine the size of the sub-patent reservoir or the required sensitivity of the PCR assay.
Furthermore, currently there is no gold standard for data collection, interpretation, and integration with current standard epidemiological approaches. Specifically, as most of the routine epidemiological indicators are reported on a monthly basis, it is not clear whether the molecular indicators would need to be collected at the same resolution, as well as the number of samples needed to calculate them. In this case, modeling can guide the design of sample collection under different assumptions about parasite and human populations and determine at what level a policy change is needed.149 Furthermore, the definition of molecular indicators is not yet established as opposed to the standard epidemiological indicators. So far, complexity of infection and other measures derived from population genetics, such as heterozygosity and identity by descent, have been used to define parasite population diversity, but it is unclear how they relate to the epidemiological indicators in space and time.
To date, molecular studies are often conducted separately from other surveillance efforts and lack systematic sample collection and statistical power. Data and interpretation are reported as peer-reviewed manuscripts, often years after sample collection. For an integrated molecular surveillance program, systematic sampling and molecular analysis are recommended, as well as fast communication of results to policymakers. For example, baseline samples collected for a TES could be screened by different RDTs per WHO recommendation and the same samples typed for Pfhrp2/3 deletion. Molecular prevalence surveys could be linked to data from health centers to understand whether the number of clinical cases diagnosed by microscopy or RDT can serve as a surrogate marker to identify the spatial or temporal heterogeneity of subclinical and sub-patent infections. Specifically, these data have been included in analyses to predict the reservoir of submicroscopic infections in the community using Bayesian statistical models or log-linear regression models as previously described.150–152 These approaches could support designing targeted interventions at the community level using incidence data from routine malaria surveillance. Finally, optimal sampling strategies need to be developed within the framework of routine surveillance, and reporting and visualization tools must be developed to facilitate data access and use for policymakers.33
Several efforts are currently aimed at developing molecular surveillance systems capable of generating relevant evidence for guiding programmatic decisions. A preeminent example is a large-scale study being conducted in Mozambique during 2022–2023.153 Accordingly, a protocol is being established and implemented for three use cases, namely monitoring drug and diagnostic resistance, identifying sources of transmission, and evaluating transmission levels and the impact of interventions. Data are being collected across a wide range of malaria transmission settings, ranging from low to high levels of transmission and exploring different sampling approaches. Downstream data analyses and modeling using the data generated in the study include descriptive analyses of observed molecular data trends as well as more elaborate modeling analyses, including stratification of malaria risk and assessment of intervention impact. Several similar efforts are currently ongoing in other sub-Saharan countries such as Tanzania.154,155 Such longitudinal studies provide valuable insights for further understanding the role of molecular surveillance and its complementarity to existing routine epidemiological surveillance in guiding malaria control programs.
To facilitate incorporating molecular surveillance data into programmatic and policy decision-making, the different use cases presented here would first need to be adapted to and incorporated in routine surveillance systems. We argue that this would entail a modular organization of processes consisting of sample and data collection, storage and downstream data analysis, and iterative dialogue using generated quantitative evidence and results to guide implementation decisions (Figure 1). Depending on the use case, molecular data collection may be performed at health facilities, through community surveys, and in sentinel sites. The frequency of sampling for the current use cases could follow current WHO guidelines (e.g., every 2 years for TES and drug resistance monitoring, every year for insecticide resistance monitoring), but it could be adjusted depending on the results of data analysis (e.g., more frequent if drug resistance markers are detected). Molecular surveillance for the prospective use cases would be best suited in settings with very low malaria transmission, close to elimination and prior implementation of reactive interventions or in the event of an outbreak. Establishing reference laboratories at the country level as well as standardization of sample collection methods and centralized data storage would facilitate comparison of assays in different locations, easy access to data, and subsequent data analyses.156 Finally, a continuous dialogue between data analysts or modelers and official bodies such as the NMCPs, ministries of health, or other authorities would be crucial for leveraging the information extracted from the molecular data to inform programmatic and implementation decisions.
Current and prospective use cases of molecular surveillance and their potential integration within malaria surveillance systems. Samples and sample metadata are collected at the community level through cross-sectional surveys, at a health facility, and at sentinel sites. Anonymized samples are shipped to the reference laboratory or any laboratory performing the analysis, whereas the metadata are sent to a centralized database. Once the samples have been analyzed, the results are sent to the central malaria database and linked to the metadata. Data analysts and modelers will use this central database to analyze and model the data, producing key malaria metrics that will be accessible through surveillance dashboards to key malaria stakeholders at the national (ministry of health, National Malaria Control Program) and regional (district health authorities) levels. These data will be used by the stakeholders to target their interventions and produce policy briefs and surveillance reports.
Citation: The American Journal of Tropical Medicine and Hygiene 112, 1_Suppl; 10.4269/ajtmh.22-0749
THE ROLE OF RECENT LABORATORY INFRASTRUCTURE DEVELOPMENT
The COVID-19 pandemic has seen unprecedented, concerted efforts from the global health community in scaling up genomic surveillance in low- and middle-income countries, especially in sub-Saharan Africa. The establishment of the Africa Centers for Disease Control and Prevention (Africa CDC) Institute of Pathogen Genomics, through the Africa Pathogen Genomics Initiative, is a great example.157 Indeed, the Africa CDC and the African Society for Laboratory Medicine have partnered with public and private institutions to improve the capacity for genomic surveillance on the continent through continental and regional laboratory hubs. This has dramatically improved the equipment and reagent procurement processes, as well as equipment maintenance, by centralizing the processes. Training activities are coordinated through a few centers of excellence on the continent, providing training and external quality assurance (EQA) schemes. The malaria community needs to build on this momentum to improve molecular surveillance by scaling up activities for recommended routine surveillance use cases and providing evidence about new use cases to define their scope of use and utility in informing public health policies.
As countries are getting access to state-of-the-art techniques for molecular and genomic surveillance, training capacity needs to be increased, and strategies for sustainability and maintaining qualified staff developed. There is a need to develop target product profiles to define the minimum criteria for assay validation and acceptance for the different use cases and to implement adequate EQA schemes to allow laboratories in malaria-endemic countries to implement these new assays.158 This will not only allow for data comparison over time and between laboratories and countries but will also greatly improve quality management systems and therefore the quality of data. Improved systems for data collection and collation will be crucial to link the laboratory, clinical, and epidemiological data through data sharing platforms on national and supranational levels—the next step for an improved real-time surveillance system.
THE WAY FORWARD
The integration of molecular data into routine malaria surveillance has the potential to complement the existing malariological data and substantially improve the quality and accuracy of information generated for decision-making. However, because of the limited funding currently available for malaria control and elimination programs as well as the lack of in-country capacity for implementing the different molecular techniques, cost-effective strategies and use cases for using molecular and genetic surveillance must be developed.
In high-transmission settings, where the reduction of the burden is the first priority, there is a need to improve the quality, accuracy, and reporting of classic epidemiological data (e.g., incidence, prevalence). Three molecular use cases could complement these data: the prevalence of resistance markers for drugs, for vectors, and for Pfhrp2/3 deletions. However, their use could be optimized to provide more robust data, even though an initially substantial investment would be required.
In low-transmission and elimination settings, where the detection of each malaria infection becomes crucial for elimination programs, the integration of portable PCR devices for reactive case detection could substantially improve the surveillance system. The development of multiplex assays that can detect other pathogens on these devices should be a priority. Indeed, in Southeast Asia, where most countries are moving to elimination, only a very low number of RDTs performed on patients with fever are positive,159 making it important for malaria programs to develop a holistic approach and collaborate with other programs. Assays with panels of pathogens specific for each region would improve not only malaria case detection but also prescription practices because in most cases of negative RDT results, health workers tend to give an antibiotic to the patient, potentially impacting antibiotic resistance. Central laboratories would still perform monitoring for drug and vector resistance and Pfhrp2/3 deletion. In addition, in these settings it will be essential to monitor the impact of the different interventions on the parasite’s genetic diversity, identify sources and sinks of infections, and confirm imported cases and parasite population movements in areas of interest.160 Furthermore, serological surveys provide a highly scalable and accessible tool to assess past and recent exposures to parasites and vectors,113,114,161 allowing stratification of regions with ongoing transmission or where malaria has been eliminated. Indeed, serological assays are cheaper to implement and can be easily used for large-scale analyses. For example, serological data indicating recent exposure either to malaria vectors or parasites in an elimination setting can trigger molecular analysis to confirm local or imported infections, helping the control program to adopt the most appropriate intervention to target local transmission foci or prevent the importation of infections.
With the recent confirmation of partial artemisinin resistance in Rwanda, Uganda, and Eritrea,162 there is a need to strengthen the surveillance system for antimalarial drug resistance across the entire sub-Saharan Africa. Consecutive health facility–based cross-sectional surveys across the country could provide valuable information on the spatiotemporal distribution of markers of resistance and potentially inform the selection of the most appropriate sentinel sites for TESs. Therefore, building the capacity for control programs to analyze samples on a regular basis for these cross-sectional surveys is of paramount importance. National reference laboratories could perform the molecular assays or work in collaboration with research laboratories that already have the expertise. The assays performed should not only be limited to molecular markers of resistance but should also include PCR correction to distinguish recrudescence from new infection in TESs, Pfhrp2/3 deletion, and molecular markers of insecticide resistance. Countries should also leverage the recent capacity developed for pathogen genomic surveillance in malaria-endemic countries through the different initiatives coordinated by the Africa CDC.163 Regional centers of excellence established by the Africa CDC could provide training and develop and implement external quality control program schemes. Finally, data analysis and mathematical modeling combining epidemiological and molecular data can provide accurate and robust information through easily accessible monitoring tools to policymakers, upon which they can base their decisions when implementing tailored interventions.
CONCLUSION
Molecular surveillance can provide essential data for NMCPs, complementing the information provided by classic routine epidemiological indicators, such as case numbers, offering a more in-depth and more granular picture of transmission dynamics in space and time. Key molecular information includes the distribution of drug, diagnostic, and insecticide resistance in all transmission settings, as well as characterization of transmission chains and heterogeneity of transmission in moderate- to low-transmission settings. Although highly valuable, challenges in terms of infrastructure, data management, data analysis including mathematical modeling, and data interpretation need to be addressed in order for molecular and genetic information to be of direct use for decision-making.
ACKNOWLEDGMENTS
We thank the RBM Partnership to End Malaria, Surveillance, Monitoring, and Evaluation Reference Group, for valuable feedback throughout the development of this manuscript. In particular, we thank Arantxa Roca Feltrer and Michelle Jituboh for reading our manuscript and providing constructive comments and suggestions.
REFERENCES
- 1.↑
Bhatt S et al., 2015. The effect of malaria control on Plasmodium falciparum in Africa between 2000 and 2015. Nature 526: 207.
- 2.↑
World Health Organization, 2021. World Malaria Report 2021. Available at: https://www.who.int/publications/i/item/9789240040496. Accessed March 9, 2022.
- 3.↑
World Health Organization, 2015. Global Technical Strategy for Malaria 2016–2030. Available at: https://www.who.int/publications/i/item/9789241564991. Accessed March 9, 2022.
- 4.↑
Dehnavieh R et al., 2019. The District Health Information System (DHIS2): a literature review and meta-synthesis of its strengths and operational challenges based on the experiences of 11 countries. Health Inf Manag 48: 62–75.
- 5.↑
World Health Organization, 2017. A Framework for Malaria Elimination. Available at: https://www.who.int/publications/i/item/9789241511988. Accessed March 1, 2022.
- 6.↑
Ozodiegwu ID, Ambrose M, Battle KE, Bever C, Diallo O, Galatas B, Runge M, Gerardin J, 2021. Beyond national indicators: adapting the Demographic and Health Surveys’ sampling strategies and questions to better inform subnational malaria intervention policy. Malar J 20: 122.
- 7.↑
Alegana VA, Okiro EA, Snow RW, 2020. Routine data for malaria morbidity estimation in Africa: challenges and prospects. BMC Med 18: 121.
- 8.↑
World Health Organization, 2018. Analysis and Use of Health Facility Data: Guidance for Malaria Programme Managers. Available at: https://www.who.int/publications/m/item/analysis-and-use-of-health-facility-data-guidancefor-malaria-programme-managers. Accessed February 1, 2022.
- 9.↑
Cohen JM, Le Menach A, Pothin E, Eisele TP, Gething PW, Eckhoff PA, Moonen B, Schapira A, Smith DL, 2017. Mapping multiple components of malaria risk for improved targeting of elimination interventions. Malar J 16: 459.
- 10.↑
Neafsey DE, Taylor AR, MacInnis BL, 2021. Advances and opportunities in malaria population genomics. Nat Rev Genet 22: 502–517.
- 11.↑
World Health Organization, 2019. Technical Consultation on the Role of Parasite and Anopheline Genetics in Malaria Surveillance. Available at: https://www.who.int/malaria/mpac/mpac-october2019-session7-report-consultation-on-genomics.pdf. Accessed March 9, 2022.
- 12.↑
Inzaule SC, Tessema SK, Kebede Y, Ouma AEO, Nkengasong JN, 2021. Genomic-informed pathogen surveillance in Africa: opportunities and challenges. Lancet Infect Dis 21: e281–e289.
- 13.↑
Dalmat R, Naughton B, Kwan-Gett TS, Slyker J, Stuckey EM, 2019. Use cases for genetic epidemiology in malaria elimination. Malar J 18: 163.
- 14.↑
Tessema SK, Raman J, Duffy CW, Ishengoma DS, Amambua-Ngwa A, Greenhouse B, 2019. Applying next-generation sequencing to track falciparum malaria in sub-Saharan Africa. Malar J 18: 268.
- 15.↑
World Health Organization, 2009. Methods for Surveillance of Antimalarial Drug Efficacy. Available at: https://www.who.int/publications/i/item/9789241597531. Accessed March 9, 2022.
- 16.↑
World Health Organization, 2016. Test Procedures for Insecticide Resistance Monitoring in Malaria Vector Mosquitoes, 2nd ed. Available at: https://apps.who.int/iris/bitstream/handle/10665/250677/9789241511575-eng.pdf. Accessed March 9, 2022.
- 17.↑
World Health Organization, 2020. Master Protocol for Surveillance of pfhrp2. Available at: https://apps.who.int/iris/bitstream/handle/10665/331197/9789240002050-eng.pdf. Accessed March 9, 2022.
- 18.↑
WHO, 2020. Report on Antimalarial Drug Efficacy, Resistance and Response: 10 Years of Surveillance (2010–2019). Geneva, Switzerland: World Health Organization, 78.
- 19.↑
WHO, 2022. Malaria Chemoprevention Efficacy Study Protocol. Geneva, Switzerland: World Health Organization, 32.
- 20.↑
Snounou G, Beck H, 1998. The use of PCR genotyping in the assessment of recrudescence or reinfection after antimalarial drug treatment. Parasitol Today 14: 462–467.
- 21.↑
World Health Organization, 2021. Informal Consultation on Methodology to Distinguish Reinfection from Recrudescence in High Malaria Transmission Areas: Report of a Virtual Meeting, 17–18 May 2021. Available at: https://www.who.int/publications/i/item/9789240038363. Accessed March 9, 2022.
- 22.↑
Messerli C, Hofmann NE, Beck H-P, Felger I, 2017. Critical evaluation of molecular monitoring in malaria drug efficacy trials and pitfalls of length-polymorphic markers. Antimicrob Agents Chemother 61: e01500–e01516.
- 23.↑
Mwingira F, Nkwengulila G, Schoepflin S, Sumari D, Beck H-P, Snounou G, Felger I, Olliaro P, Mugittu K, 2011. Plasmodium falciparum msp1, msp2 and glurp allele frequency and diversity in sub-Saharan Africa. Malar J 10: 79.
- 24.↑
Koepfli C, Schoepflin S, Bretscher M, Lin E, Kiniboro B, Zimmerman PA, Siba P, Smith TA, Mueller I, Felger I, 2011. How much remains undetected? Probability of molecular detection of human Plasmodia in the field. PLoS One 6: e19010.
- 25.↑
Barry A, Awandu SS, Tiono AB, Grignard L, Bousema T, Collins KA, 2021. Improved detectability of Plasmodium falciparum clones with repeated sampling in incident and chronic infections in Burkina Faso. Am J Trop Med Hyg 106: 664–666.
- 26.↑
Plucinski MM, Morton L, Bushman M, Dimbu PR, Udhayakumar V, 2015. Robust algorithm for systematic classification of malaria late treatment failures as recrudescence or reinfection using microsatellite genotyping. Antimicrob Agents Chemother 59: 6096–6100.
- 27.↑
Jones S, Kay K, Hodel E, Chy S, Mbituyumuremyi A, Uwimana A, Menard D, Felger I, Hastings I, 2019. Improving methods for analyzing antimalarial drug efficacy trials: molecular correction based on length-polymorphic markers msp-1, msp-2, and glurp. Antimicrob Agents Chemother 63: e00590-19.
- 28.↑
Jones S, Plucinski M, Kay K, Hodel EM, Hastings IM, 2020. A computer modelling approach to evaluate the accuracy of microsatellite markers for classification of recurrent infections during routine monitoring of antimalarial drug efficacy. Antimicrob Agents Chemother 64: e01517–e01519.
- 29.↑
Jones S, Kay K, Hodel EM, Gruenberg M, Lerch A, Felger I, Hastings I, 2021. Should deep-sequenced amplicons become the new gold standard for analyzing malaria drug clinical trials? Antimicrob Agents Chemother 65: e00437 21.
- 30.↑
Gruenberg M, Lerch A, Beck H-P, Felger I, 2019. Amplicon deep sequencing improves Plasmodium falciparum genotyping in clinical trials of antimalarial drugs. Sci Rep 9: 17790.
- 31.↑
Lerch A, Koepfli C, Hofmann NE, Messerli C, Wilcox S, Kattenberg JH, Betuela I, O’Connor L, Mueller I, Felger I, 2017. Development of amplicon deep sequencing markers and data analysis pipeline for genotyping multi-clonal malaria infections. BMC Genomics 18: 864.
- 32.↑
Ataide R et al., 2017. Host immunity to Plasmodium falciparum and the assessment of emerging artemisinin resistance in a multinational cohort. Proc Natl Acad Sci USA 114: 3515–3520.
- 33.↑
Nsanzabana C, 2021. Time to scale up molecular surveillance for anti-malarial drug resistance in sub-Saharan Africa. Malar J 20: 401.
- 34.↑
Nsanzabana C, 2019. Resistance to artemisinin combination therapies (ACTs): do not forget the partner drug! Trop Med Infect Dis 4: 26.
- 35.↑
Leang R et al., 2015. Evidence of Plasmodium falciparum malaria multidrug resistance to artemisinin and piperaquine in Western Cambodia: dihydroartemisinin-piperaquine open-label multicenter clinical assessment. Antimicrob Agents Chemother 59: 4719–4726.
- 36.↑
Uwimana A et al., 2021. Association of Plasmodium falciparum kelch13 R561H genotypes with delayed parasite clearance in Rwanda: an open-label, single-arm, multicentre, therapeutic efficacy study. Lancet Infect Dis 21: 1120–1128.
- 37.↑
Ashley EA et al., 2014. Spread of artemisinin resistance in Plasmodium falciparum malaria. N Engl J Med 371: 411–423.
- 38.↑
Balikagala B et al., 2021. Evidence of artemisinin-resistant malaria in Africa. N Engl J Med 385: 1163–1171.
- 39.↑
Imwong M et al., 2017. The spread of artemisinin-resistant Plasmodium falciparum in the Greater Mekong subregion: a molecular epidemiology observational study. Lancet Infect Dis 17: 491–497.
- 40.↑
Imwong M et al., 2021. Evolution of multidrug resistance in Plasmodium falciparum: a longitudinal study of genetic resistance markers in the Greater Mekong subregion. Antimicrob Agents Chemother 65: e0112121.
- 41.↑
Nsanzabana C et al., 2018. Molecular assays for antimalarial drug resistance surveillance: a target product profile. PLoS One 13: e0204347.
- 42.↑
Plowe CV et al., 2007. World Antimalarial Resistance Network (WARN) III: molecular markers for drug resistant malaria. Malar J 6: 121.
- 43.↑
Mugittu K et al., 2004. Therapeutic efficacy of sulfadoxine-pyrimethamine and prevalence of resistance markers in Tanzania prior to revision of malaria treatment policy: Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase mutations in monitoring in vivo resistance. Am J Trop Med Hyg 71: 696.
- 44.↑
Djimdé A et al., 2001. A molecular marker for chloroquine-resistant falciparum malaria. N Engl J Med 344: 257–263.
- 45.↑
Djimdé A, Doumbo OK, Steketee RW, Plowe CV, 2001. Application of a molecular marker for surveillance of chloroquine-resistant falciparum malaria. Lancet 358: 890–891.
- 46.↑
Nsanzabana C et al., 2010. Quantifying the evolution and impact of antimalarial drug resistance: drug use, spread of resistance, and drug failure over a 12-year period in Papua New Guinea. J Infect Dis 201: 435–443.
- 47.↑
Bretscher MT et al., 2020. The duration of chemoprophylaxis against malaria after treatment with artesunate-amodiaquine and artemether-lumefantrine and the effects of pfmdr1 86Y and pfcrt 76T: a meta-analysis of individual patient data. BMC Med 18: 47.
- 48.↑
Venkatesan M et al., 2014. Polymorphisms in Plasmodium falciparum chloroquine resistance transporter and multidrug resistance 1 genes: parasite risk factors that affect treatment outcomes for P. falciparum malaria after artemether-lumefantrine and artesunate-amodiaquine. Am J Trop Med Hyg 91: 833.
- 49.↑
Ehrlich HY, Bei AK, Weinberger DM, Warren JL, Parikh S, 2021. Mapping partner drug resistance to guide antimalarial combination therapy policies in sub-Saharan Africa. Proc Natl Acad Sci USA 118: e2100685118.
- 50.↑
Ehrlich HY, Jones J, Parikh S, 2020. Molecular surveillance of antimalarial partner drug resistance in sub-Saharan Africa: a spatial-temporal evidence mapping study. Lancet Microbe 1: e209–e217.
- 51.↑
Incardona S, Serra-Casas E, Champouillon N, Nsanzabana C, Cunningham J, González IJ, 2017. Global survey of malaria rapid diagnostic test (RDT) sales, procurement and lot verification practices: assessing the use of the WHO–FIND Malaria RDT Evaluation Programme (2011–2014). Malar J 16: 196.
- 52.↑
Mayor A, Bassat Q, 2019. “Resistance” to diagnostics: a serious biological challenge for malaria control and elimination. EBioMedicine 50: 9–10.
- 53.↑
Agaba BB et al., 2019. Systematic review of the status of pfhrp2 and pfhrp3 gene deletion, approaches and methods used for its estimation and reporting in Plasmodium falciparum populations in Africa: review of published studies 2010–2019. Malar J 18: 355.
- 54.↑
Niyukuri D et al., 2022. High sensitivity of a novel rapid test for the diagnosis of clinical and subclinical Plasmodium falciparum infections in a high transmission setting in Burundi. PLOS Glob Public Health 2: e0000828.
- 55.↑
Gamboa D et al., 2010. A large proportion of P. falciparum isolates in the Amazon region of Peru lack pfhrp2 and pfhrp3: implications for malaria rapid diagnostic tests. PLoS One 5: e8091.
- 56.↑
Thomson R, Parr JB, Cheng Q, Chenet S, Perkins M, Cunningham J, 2020. Prevalence of Plasmodium falciparum lacking histidine-rich proteins 2 and 3: a systematic review. Bull World Health Organ 98: 558–568F.
- 57.↑
Poti KE, Sullivan DJ, Dondorp AM, Woodrow CJ, 2020. HRP2: transforming malaria diagnosis, but with caveats. Trends Parasitol 36: 112–126.
- 58.↑
Berhane A et al., 2018. Major threat to malaria control programs by Plasmodium falciparum lacking histidine-rich protein 2, Eritrea. Emerg Infect Dis 24: 462.
- 59.↑
Golassa L, Messele A, Amambua-Ngwa A, Swedberg G, 2020. High prevalence and extended deletions in Plasmodium falciparum hrp2/3 genomic loci in Ethiopia. PLoS One 15: e0241807.
- 60.↑
Feleke SM et al., 2021. Plasmodium falciparum is evolving to escape malaria rapid diagnostic tests in Ethiopia. Nat Microbiol 6: 1289–1299.
- 61.↑
World Health Organization, 2019. Response Plan to pfhrp2 Gene Deletions. Available at: https://apps.who.int/iris/bitstream/handle/10665/325528/WHO-CDS-GMP-2019.02-eng.pdf. Accessed September 1, 2022.
- 62.↑
Schindler T et al., 2019. A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum. Sci Rep 9: 13107.
- 63.↑
Grignard L et al., 2020. A novel multiplex qPCR assay for detection of Plasmodium falciparum with histidine-rich protein 2 and 3 (pfhrp2 and pfhrp3) deletions in polyclonal infections. EBioMedicine 55: 102757.
- 64.↑
Kreidenweiss A et al., 2019. Monitoring the threatened utility of malaria rapid diagnostic tests by novel high-throughput detection of Plasmodium falciparum hrp2 and hrp3 deletions: a cross-sectional, diagnostic accuracy study. EBioMedicine 50: 14–22.
- 65.↑
Vera-Arias CA et al., 2022. High-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacy. eLife 11: e72083.
- 66.↑
World Health Organization, 2022. Manual for Monitoring Insecticide Resistance in Mosquito Vectors and Selecting Appropriate Interventions. Available at: https://www.who.int/publications/i/item/9789240051089. Accessed September 5, 2022.
- 67.↑
Owusu HF, Jancaryova D, Malone D, Muller P, 2015. Comparability between insecticide resistance bioassays for mosquito vectors: time to review current methodology? Parasit Vectors 8: 357.
- 68.↑
Matiya DJ, Philbert AB, Kidima W, Matowo JJ, 2019. Dynamics and monitoring of insecticide resistance in malaria vectors across mainland Tanzania from 1997 to 2017: a systematic review. Malar J 18: 102.
- 69.↑
Vontas J, Mavridis K, 2019. Vector population monitoring tools for insecticide resistance management: myth or fact? Pestic Biochem Physiol 161: 54–60.
- 70.↑
Namias A, Jobe NB, Paaijmans KP, Huijben S, 2021. The need for practical insecticide-resistance guidelines to effectively inform mosquito-borne disease control programs. eLife 10: e65655.
- 71.↑
Donnelly MJ, Isaacs AT, Weetman D, 2016. Identification, validation, and application of molecular diagnostics for insecticide resistance in malaria vectors. Trends Parasitol 32: 197–206.
- 72.↑
Owusu HF, Jančáryová D, Malone D, Müller P, 2015. Comparability between insecticide resistance bioassays for mosquito vectors: time to review current methodology? Parasit Vectors 8: 357.
- 73.↑
Richards SL, Byrd BD, Reiskind MH, White AV, 2020. Assessing insecticide resistance in adult mosquitoes: perspectives on current methods. Environ Health Insights 14: 1178630220952790.
- 74.↑
Kushwah RBS, Kaur T, Dykes CL, Ravi Kumar H, Kapoor N, Singh OP, 2020. A new knockdown resistance (kdr) mutation, F1534L, in the voltage-gated sodium channel of Aedes aegypti, co-occurring with F1534C, S989P and V1016G. Parasit Vectors 13: 327.
- 75.↑
Lol JC, Castañeda D, Mackenzie-Impoinvil L, Romero CG, Lenhart A, Padilla NR, 2019. Development of molecular assays to detect target-site mechanisms associated with insecticide resistance in malaria vectors from Latin America. Malar J 18: 202.
- 76.↑
Silva R, Mavridis K, Vontas J, Rodrigues A, Osório HC, 2020. Monitoring and molecular profiling of contemporary insecticide resistance status of malaria vectors in Guinea–Bissau. Acta Trop 206: 105440.
- 77.↑
Granada Y, Mejía-Jaramillo AM, Zuluaga S, Triana-Chávez O, 2021. Molecular surveillance of resistance to pyrethroids insecticides in Colombian Aedes aegypti populations. PLoS Negl Trop Dis 15: e0010001.
- 78.↑
Yin J, Yamba F, Zheng C, Zhou S, Smith SJ, Wang L, Li H, Xia Z, Xiao N, 2021. Molecular detection of insecticide resistance mutations in Anopheles gambiae from Sierra Leone using multiplex SNaPshot and sequencing. Front Cell Infect Microbiol 11: 666469.
- 79.↑
Zhao N et al., 2021. Entomological and molecular surveillance of Anopheles mosquitoes in Freetown, Sierra Leone, 2019. Front Public Health 9: 649672.
- 80.↑
Sarkar M, Borkotoki A, Baruah I, Bhattacharyya IK, Srivastava RB, 2009. Molecular analysis of knock down resistance (kdr) mutation and distribution of kdr genotypes in a wild population of Culex quinquefasciatus from India. Trop Med Int Health 14: 1097–1104.
- 81.↑
Donnelly MJ, Isaacs AT, Weetman D, 2016. Identification, validation, and application of molecular diagnostics for insecticide resistance in malaria vectors. Trends Parasitol 32: 197–206.
- 82.↑
Samuels AM et al., 2021. Impact of community-based mass testing and treatment on malaria infection prevalence in a high-transmission area of western Kenya: a cluster randomized controlled trial. Clin Infect Dis 72: 1927–1935.
- 83.↑
McCreesh P et al., 2018. Subpatent malaria in a low transmission African setting: a cross-sectional study using rapid diagnostic testing (RDT) and loop-mediated isothermal amplification (LAMP) from Zambezi region, Namibia. Malar J 17: 480.
- 84.↑
Ranadive N et al., 2017. Limitations of rapid diagnostic testing in patients with suspected malaria: a diagnostic accuracy evaluation from Swaziland, a low-endemicity country aiming for malaria elimination. Clin Infect Dis 64: 1221–1227.
- 85.↑
Okell LC, Ghani AC, Lyons E, Drakeley CJ, 2009. Submicroscopic infection in Plasmodium falciparum-endemic populations: a systematic review and meta-analysis. J Infect Dis 200: 1509–1517.
- 86.↑
Cheng Q, Cunningham J, Gatton ML, 2015. Systematic review of sub-microscopic P. vivax infections: prevalence and determining factors. PLoS Negl Trop Dis 9: e3413.
- 87.↑
Inglis TJ, Bradbury RS, McInnes RL, Frances SP, Merritt AJ, Levy A, Nicholson J, Neville PJ, Lindsay M, Smith DW, 2016. Deployable molecular detection of arboviruses in the Australian outback. Am J Trop Med Hyg 95: 633–638.
- 88.↑
Faust CL et al., 2021. Harnessing technology and portability to conduct molecular epidemiology of endemic pathogens in resource-limited settings. Trans R Soc Trop Med Hyg 115: 3–5.
- 89.↑
Carlier L, Baker SC, Huwe T, Yewhalaw D, Haileselassie W, Koepfli C, 2022. qPCR in a suitcase for rapid Plasmodium falciparum and Plasmodium vivax surveillance in Ethiopia. PLOS Glob Public Health 2: e0000454.
- 90.↑
Kyaw SS et al., 2021. Estimating the programmatic cost of targeted mass drug administration for malaria in Myanmar. BMC Public Health 21: 826.
- 91.↑
Zhou S-S et al., 2015. China’s 1-3-7 surveillance and response strategy for malaria elimination: is case reporting, investigation and foci response happening according to plan? Infect Dis Poverty 4: 55.
- 92.↑
Premaratne R, Wickremasinghe R, Ranaweera D, Gunasekera W, Hevawitharana M, Pieris L, Fernando D, Mendis K, 2019. Technical and operational underpinnings of malaria elimination from Sri Lanka. Malar J 18: 256.
- 93.↑
Leslie T, Nahzat S, Sediqi W, 2016. Epidemiology and control of Plasmodium vivax in Afghanistan. Am J Trop Med Hyg 95: 72.
- 94.↑
Von Seidlein L et al., 2019. The impact of targeted malaria elimination with mass drug administrations on falciparum malaria in Southeast Asia: a cluster randomised trial. PLoS Med 16: e1002745.
- 95.↑
Salgado C, Ayodo G, Macklin MD, Gould MP, Nallandhighal S, Odhiambo EO, Obala A, O’Meara WP, John CC, Tran TM, 2021. The prevalence and density of asymptomatic Plasmodium falciparum infections among children and adults in three communities of western Kenya. Malar J 20: 371.
- 96.↑
Koepfli C et al., 2017. Sustained malaria control over an 8-year period in Papua New Guinea: the challenge of low-density asymptomatic Plasmodium infections. J Infect Dis 216: 1434–1443.
- 97.↑
Grossenbacher B, Holzschuh A, Hofmann NE, Omar KA, Stuck L, Fakih BS, Ali A, Yukich J, Hetzel MW, Felger I, 2020. Molecular methods for tracking residual Plasmodium falciparum transmission in a close-to-elimination setting in Zanzibar. Malar J 19: 50.
- 98.↑
Hergott DE et al., 2022. Feasibility of community at-home dried blood spot collection combined with pooled reverse transcription PCR as a viable and convenient method for malaria epidemiology studies. Malar J 21: 221.
- 99.↑
Rahi M, Sharma R, Saroha P, Chaturvedi R, Bharti PK, Sharma A, 2022. Polymerase chain reaction-based malaria diagnosis can be increasingly adopted during current phase of malaria elimination in India. Am J Trop Med Hyg 106: 1005–1012.
- 100.↑
Lopez L, Koepfli C, 2021. Systematic review of Plasmodium falciparum and Plasmodium vivax polyclonal infections: impact of prevalence, study population characteristics, and laboratory procedures. PLoS One 16: e0249382.
- 101.↑
Koepfli C, Mueller I, 2017. Malaria epidemiology at the clone level. Trends Parasitol 33: 974–985.
- 102.↑
Noviyanti R et al., 2020. Implementing parasite genotyping into national surveillance frameworks: feedback from control programmes and researchers in the Asia-Pacific region. Malar J 19: 271.
- 103.↑
Holzschuh A et al., 2023. Multiplexed ddPCR-amplicon sequencing reveals isolated Plasmodium falciparum populations amenable to local elimination in Zanzibar, Tanzania. Nat Commun 14: 3699.
- 104.↑
Wesolowski A, Taylor AR, Chang HH, Verity R, Tessema S, Bailey JA, Alex Perkins T, Neafsey DE, Greenhouse B, Buckee CO, 2018. Mapping malaria by combining parasite genomic and epidemiologic data. BMC Med 16: 190.
- 105.↑
Tessema S et al., 2019. Using parasite genetic and human mobility data to infer local and cross-border malaria connectivity in Southern Africa. eLife 8: e43510.
- 106.↑
Chang H-H et al., 2019. Mapping imported malaria in Bangladesh using parasite genetic and human mobility data. eLife 8: e43481.
- 107.↑
Sy M et al., 2022. Plasmodium falciparum genomic surveillance reveals spatial and temporal trends, association of genetic and physical distance, and household clustering. Sci Rep 12: 938.
- 108.↑
Searle KM, Katowa B, Kobayashi T, Siame MNS, Mharakurwa S, Carpi G, Norris DE, Stevenson JC, Thuma PE, Moss WJ, Southern Africa International Centers of Excellence for Malaria Research, 2017. Distinct parasite populations infect individuals identified through passive and active case detection in a region of declining malaria transmission in southern Zambia. Malar J 16: 154.
- 109.↑
Tessema S et al., 2019. Using parasite genetic and human mobility data to infer local and cross-border malaria connectivity in Southern Africa. eLife 8: e43510.
- 110.↑
Drakeley C et al., 2005. Estimating medium-and long-term trends in malaria transmission by using serological markers of malaria exposure. Proc Natl Acad Sci USA 102: 5108–5113.
- 111.↑
Wu L et al., 2020. Antibody responses to a suite of novel serological markers for malaria surveillance demonstrate strong correlation with clinical and parasitological infection across seasons and transmission settings in the Gambia. BMC Med 18: 304.
- 112.↑
Stevenson JC, Stresman GH, Baidjoe A, Okoth A, Oriango R, Owaga C, Marube E, Bousema T, Cox J, Drakeley C, 2015. Use of different transmission metrics to describe malaria epidemiology in the highlands of western Kenya. Malar J 14: 418.
- 113.↑
Druetz T et al., 2022. Etramp5 as a useful serological marker in children to assess the immediate effects of mass drug campaigns for malaria. BMC Infect Dis 22: 643.
- 114.↑
van den Hoogen LL et al., 2020. Selection of antibody responses associated with Plasmodium falciparum Infections in the context of malaria elimination. Front Immunol 11: 928.
- 115.↑
Poinsignon A, Cornelie S, Ba F, Boulanger D, Sow C, Rossignol M, Sokhna C, Cisse B, Simondon F, Remoue F, 2009. Human IgG response to a salivary peptide, gSG6-P1, as a new immuno-epidemiological tool for evaluating low-level exposure to Anopheles bites. Malar J 8: 198.
- 116.↑
Ya-Umphan P, Cerqueira D, Parker DM, Cottrell G, Poinsignon A, Remoue F, Brengues C, Chareonviriyaphap T, Nosten F, Corbel V, 2017. Use of an Anopheles salivary biomarker to assess malaria transmission risk along the Thailand-Myanmar border. J Infect Dis 215: 396–404.
- 117.↑
Badu K, Siangla J, Larbi J, Lawson BW, Afrane Y, Ong’echa J, Remoue F, Zhou G, Githeko AK, Yan G, 2012. Variation in exposure to Anopheles gambiae salivary gland peptide (gSG6-P1) across different malaria transmission settings in the western Kenya highlands. Malar J 11: 318.
- 118.↑
Sagna AB, Kibria MG, Naher S, Islam S, Aktaruzzaman M, Alam MS, Koepfli C, 2020. Stratifying malaria receptivity in Bangladesh using archived rapid diagnostic tests. Malar J 19: 345.
- 119.↑
Chaudhury S, Bolton JS, Eller LA, Robb M, Ake J, Ngauy V, Regules JA, Kamau E, Bergmann-Leitner ES, 2022. Assessing prevalence and transmission rates of malaria through simultaneous profiling of antibody responses against Plasmodium and Anopheles antigens. J Clin Med 11: 1839.
- 120.↑
Crompton PD et al., 2010. A prospective analysis of the Ab response to Plasmodium falciparum before and after a malaria season by protein microarray. Proc Natl Acad Sci USA 107: 6958–6963.
- 121.↑
Mohanty A, Swain S, Singh DV, Mahapatra N, Kar SK, Hazra RK, 2009. A unique methodology for detecting the spread of chloroquine-resistant strains of Plasmodium falciparum, in previously unreported areas, by analyzing anophelines of malaria endemic zones of Orissa, India. Infect Genet Evol 9: 462–467.
- 122.↑
Smith-Aguasca R, Gupta H, Uberegui E, Maquina M, Saute F, Paaijmans KP, Mayor A, Huijben S, 2019. Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by next generation sequencing of Plasmodium falciparum. Malar J 18: 351.
- 123.↑
Maude RJ, Lubell Y, Socheat D, Yeung S, Saralamba S, Pongtavornpinyo W, Cooper BS, Dondorp AM, White NJ, White LJ, 2010. The role of mathematical modelling in guiding the science and economics of malaria elimination. Int Health 2: 239–246.
- 124.↑
Alonso PL et al., 2011. A research agenda to underpin malaria eradication. PLoS Med 8: e1000406.
- 125.↑
malERA Refresh Consultative Panel on Combination Interventions and Modelling, 2017. malERA: an updated research agenda for combination interventions and modelling in malaria elimination and eradication. PLoS Med 14: e1002453.
- 126.↑
Runge M et al., 2020. Simulating the council-specific impact of anti-malaria interventions: a tool to support malaria strategic planning in Tanzania. PLoS One 15: e0228469.
- 127.↑
Ghilardi L et al., 2020. How useful are malaria risk maps at the country level? Perceptions of decision-makers in Kenya, Malawi and the Democratic Republic of Congo. Malar J 19: 353.
- 128.↑
Thawer SG et al., 2020. Sub-national stratification of malaria risk in mainland Tanzania: a simplified assembly of survey and routine data. Malar J 19: 177.
- 129.↑
Odhiambo JN, Kalinda C, Macharia PM, Snow RW, Sartorius B, 2020. Spatial and spatio-temporal methods for mapping malaria risk: a systematic review. BMJ Glob Health 5: e002919.
- 130.↑
Runge M, Molteni F, Mandike R, Snow RW, Lengeler C, Mohamed A, Pothin E, 2020. Applied mathematical modelling to inform national malaria policies, strategies and operations in Tanzania. Malar J 19: 101.
- 131.↑
The Global Fund, 2013. The Global Fund’s New Funding Model. Available at: https://www.theglobalfund.org/media/1467/replenishment_2013newfundingmodel_report_en.pdf. Accessed March 1, 2022.
- 132.↑
Phillips DE et al., 2020. Bringing a health systems modelling approach to complex evaluations: multicountry applications in HIV, TB and malaria. BMJ Glob Health 5: e002441.
- 133.↑
Feng X-Y, Xia Z-G, Vong S, Yang W-Z, Zhou S-S, 2014. Surveillance and response to drive the national malaria elimination program. Adv Parasitol 86: 81–108.
- 134.↑
Daniels RF et al., 2015. Modeling malaria genomics reveals transmission decline and rebound in Senegal. Proc Natl Acad Sci USA 112: 7067–7072.
- 135.↑
Watson OJ et al., 2021. Evaluating the performance of malaria genetics for inferring changes in transmission intensity using transmission modeling. Mol Biol Evol 38: 274–289.
- 136.↑
Watson OJ, Slater HC, Verity R, Parr JB, Mwandagalirwa MK, Tshefu A, Meshnick SR, Ghani AC, 2017. Modelling the drivers of the spread of Plasmodium falciparum hrp2 gene deletions in sub-Saharan Africa. eLife 6: e25008.
- 137.↑
Masserey T, Lee T, Golumbeanu M, Shattock AJ, Kelly SL, Hastings IM, Penny MA, 2022. The influence of biological, epidemiological, and treatment factors on the establishment and spread of drug-resistant Plasmodium falciparum. eLife 11: e77634.
- 138.↑
Watson OJ, Gao B, Nguyen TD, Tran TN-A, Penny MA, Smith DL, Okell L, Aguas R, Boni MF, 2021. Pre-existing partner-drug resistance facilitates the emergence and spread of artemisinin resistance: a consensus modelling study. Lancet Microbe 3: e701–e710.
- 139.↑
Sy M et al., 2021. Genomic investigation of atypical malaria cases in Kanel, northern Senegal. Malar J 20: 103.
- 140.↑
Hastings IM, Felger I, 2022. WHO antimalarial trial guidelines: good science, bad news? Trends Parasitol 38: 933–941.
- 141.↑
Weiss DJ et al., 2019. Mapping the global prevalence, incidence, and mortality of Plasmodium falciparum, 2000–17: a spatial and temporal modelling study. Lancet 394: 322–331.
- 142.↑
Battle KE et al., 2019. Mapping the global endemicity and clinical burden of Plasmodium vivax, 2000–17: a spatial and temporal modelling study. Lancet 394: 332–343.
- 143.↑
Hodoameda P, Duah-Quashie NO, Quashie NB, 2022. Assessing the roles of molecular markers of antimalarial drug resistance and the host pharmacogenetics in drug-resistant malaria. J Trop Med 2022: 3492696.
- 144.↑
Ippolito MM, Moser KA, Kabuya J-BB, Cunningham C, Juliano JJ, 2021. Antimalarial drug resistance and implications for the WHO global technical strategy. Curr Epidemiol Rep 8: 46–62.
- 145.↑
Ishengoma DS, Saidi Q, Sibley CH, Roper C, Alifrangis M, 2019. Deployment and utilization of next-generation sequencing of Plasmodium falciparum to guide anti-malarial drug policy decisions in sub-Saharan Africa: opportunities and challenges. Malar J 18: 267.
- 146.↑
Nguyen TD, Tran TN-A, Parker DM, White NJ, Boni MF, 2021. Antimalarial mass drug administration in large populations and the evolution of drug resistance. PLOS Glob Public Health 3: e0002200.
- 147.↑
Nguyen TD, Gao B, Amaratunga C, Dhorda M, Tran TN-A, White NJ, Dondorp AM, Boni MF, Aguas R, 2022. Preventing antimalarial drug resistance with triple artemisinin-based combination therapies. Nat Commun 14: 4568.
- 148.↑
Zupko RJ, Nguyen TD, Ngabonziza JCS, Kabera M, Li H, Tran TN-A, Tran KT, Uwimana A, Boni MF, 2022. Potential policy interventions for slowing the spread of artemisinin-resistant pfkelch R561H mutations in Rwanda. Nat Med: doi: 10.1038/s41591-023-02551-w.
- 149.↑
Mayor A, Ishengoma DS, Proctor JL, Verity R, 2022. Sampling for malaria molecular surveillance. Trends Parasitol 39: 954–968.
- 150.↑
Cameron E et al., 2015. Defining the relationship between infection prevalence and clinical incidence of Plasmodium falciparum malaria. Nat Commun 6: 8170.
- 151.↑
Patil AP, Okiro EA, Gething PW, Guerra CA, Sharma SK, Snow RW, Hay SI, 2009. Defining the relationship between Plasmodium falciparum parasite rate and clinical disease: statistical models for disease burden estimation. Malar J 8: 186.
- 152.↑
Stresman G et al., 2020. Association between the proportion of Plasmodium falciparum and Plasmodium vivax infections detected by passive surveillance and the magnitude of the asymptomatic reservoir in the community: a pooled analysis of paired health facility and community data. Lancet Infect Dis 20: 953–963.
- 153.↑
Mayor A et al., 2022. Prospective surveillance study to detect antimalarial drug resistance, gene deletions of diagnostic relevance and genetic diversity of Plasmodium falciparum in Mozambique: protocol. BMJ Open 12: e063456.
- 154.↑
Moser KA et al., 2021. Describing the current status of Plasmodium falciparum population structure and drug resistance within mainland Tanzania using molecular inversion probes. Mol Ecol 30: 100–113.
- 155.↑
Lyimo BM et al., 2022. Potential opportunities and challenges of deploying next generation sequencing and CRISPR-Cas systems to support diagnostics and surveillance towards malaria control and elimination in Africa. Front Cell Infect Microbiol 12: 757844.
- 156.↑
Nsanzabana C, 2019. Strengthening surveillance systems for malaria elimination by integrating molecular and genomic data. Trop Med Infect Dis 4: 139.
- 157.↑
Wilkinson E et al., 2021. A year of genomic surveillance reveals how the SARS-CoV-2 pandemic unfolded in Africa. Science 374: 423–431.
- 158.↑
Nsanzabana C et al., 2018. Molecular assays for antimalarial drug resistance surveillance: a target product profile. PLoS One 13: e0204347.
- 159.↑
Lubell Y et al., 2019. Economic considerations support C-reactive protein testing alongside malaria rapid diagnostic tests to guide antimicrobial therapy for patients with febrile illness in settings with low malaria endemicity. Malar J 18: 442.
- 160.↑
Wasakul V et al., 2022. Malaria outbreak in Laos driven by a selective sweep for Plasmodium falciparum kelch13 R539T mutants: a genetic epidemiology analysis. Lancet Infect Dis 23: 568–577.
- 161.↑
Chaudhury S, Bolton JS, Eller LA, Robb M, Ake J, Ngauy V, Regules JA, Kamau E, Bergmann-Leitner ES, 2022. Assessing prevalence and transmission rates of malaria through simultaneous profiling of antibody responses against Plasmodium and Anopheles antigens. J Clin Med 11: 1839.
- 162.↑
World Health Organization, 2022. Draft Strategy to Respond to Antimalarial Drug Resistance in Africa. Available at: https://www.who.int/publications/i/item/9789240060265. Accessed August 11, 2022.
- 163.↑
Inzaule SC, Tessema SK, Kebede Y, Ogwell Ouma AE, Nkengasong JN, 2021. Genomic-informed pathogen surveillance in Africa: opportunities and challenges. Lancet Infect Dis 21: e281–e289.