We acknowledge the staff and patients at Masafu Hospital in Busia, Uganda, for their support in carrying out the study and willingness to be a part of research efforts to create better diagnostic tests for malaria. We are also grateful to Access Bio for manufacturing the prototype rapid diagnostic tests, and to Jun Yong Ha, Director of R&D at Access Bio, for his technical collaboration on developing the test. Special thanks to additional developers of the assay at Global Good for their innovative approach to malaria diagnostics: Veronika V. Glukhova, John Williford, and Barbara Barrios-Lopez.
World Health Organization , 2020. World Malaria Report 2020. Geneva, Switzerland, WHO.
Hofmann NE et al.2019. Diagnostic performance of conventional and ultrasensitive rapid diagnostic tests for malaria in febrile outpatients in Tanzania. J Infect Dis 219: 1490–1498.
Bell D, Wongsrichanalai C, Barnwell JW , 2006. Ensuring quality and access for malaria diagnosis: how can it be achieved? Nat Rev Microbiol 4: S7–S20.
Hopkins H, Bebell L, Kambale W, Dokomajilar C, Rosenthal PJ, Dorsey G , 2008. Rapid diagnostic tests for malaria at sites of varying transmission intensity in Uganda. J Infect Dis 197: 510–518.
World Health Organization , 2018. Malaria Rapid Diagnostic Test Performance: Results of WHO Product Testing of Malaria RDTs: Round 8 (2016–2018). Geneva, Switzerland: WHO.
Gamboa D et al.2010. A large proportion of P. falciparum isolates in the Amazon region of Peru lack pfhrp2 and pfhrp3: implications for malaria rapid diagnostic tests. PLoS One 5: e8091.
Berhane A et al.2018. Major threat to malaria control programs by Plasmodium falciparum lacking histidine-rich protein 2, Eritrea. Emerg Infect Dis 24: 462–470.
Bharti PK, Chandel HS, Ahmad A, Krishna S, Udhayakumar V, Singh N , 2016. Prevalence of pfhrp2 and/or pfhrp3 gene deletion in Plasmodium falciparum population in eight highly endemic states in India. PLoS One 11: 1–16.
Houzé S, Boly MD, le Bras J, Deloron P, Faucher JF , 2009. PfHRP2 and PfLDH antigen detection for monitoring the efficacy of artemisinin-based combination therapy (ACT) in the treatment of uncomplicated falciparum malaria. Malar J 8: 2–9.
World Health Organization & UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases, 2015?. Microscopy for the Detection, Identification and Quantification of Malaria Parasites on Stained Thick and Thin Blood Films in Research Settings (?version 1.0)?: Procedure: Methods Manual. Geneva, Switzerland: World Health Organization.
Hofmann N, Mwingira F, Shekalaghe S, Robinson LJ, Mueller I, Felger I , 2015. Ultra-sensitive detection of Plasmodium falciparum by amplification of multi-copy subtelomeric targets. PLoS Med 12: e1001788.
Flahault A, Cadilhac M, Thomas G , 2005. Sample size calculation should be performed for design accuracy in diagnostic test studies. J Clin Epidemiol 58: 859–862.