We are thankful for great colleagues at IIT-Kanpur—Hari Shankar, Harish Vishwakarma, Shivshankar Mishraa, and numerous graduate students who helped with instrumentation and shared their laboratory space—and the countless people who shared their homes and their tea with us as we sampled in their community.
Vikesland PJ et al. 2017. Toward a comprehensive strategy to mitigate dissemination of environmental sources of antibiotic resistance. Environ Sci Technol 51: 13061–13069.
de J, Sosal A, Byarugaba DK, Amabile-Cuevas CF, Hsueh PR, Kariuki S, Okeke IN, eds. 2010. Antimicrobial Resistance in Developing Countries Berling/Heidelberg, Germany: Springer Science and Buisiness Media, 3–36. doi: 10.1007/s13312-014-0374-3.
Pruden A, Pei R, Storteboom H, Carlson KH, 2006. Antibiotic resistance genes as emerging contaminants: studies in northern Colorado. Environ Sci Technol 40: 7445–7450.
Laxminarayan R, Chaudhuryanan RR, 2016. Antibiotic resistance in India: drivers and opportunities for action. PLoS Med 13: e1001974. doi: 10.1371/journal.pmed.1001974.
Shahid M, Singh A, Sobia F, Rashid M, Malik A, Shukla I, Khan HM, 2011. Bla CTX-M, bla TEM, and bla SHV in Enterobacteriaceae from North-Indian tertiary hospital: high occurrence of combination genes. Asian Pac J Trop Med 4: 101–105.
Prakash S, 2006. Carbapenem sensitivity profile amongst bacterial isolates from clinical specimens in Kanpur city. Indian J Crit Care Med 10: 10–13.
Bennett PM, 2008. Plasmid encoded antibiotic resistance: acquisition and transfer of antibiotic resistance genes in bacteria. Br J Pharmacol 153: 347–357.
Schrag SJ, McGee L, Whitney CG, Beall B, Craig AS, Choate ME, Jorgensen JH, Facklam RR, Klugman KP, 2004. Emergence of Streptococcus pneumoniae with very-high-level resistance to penicillin. Antimicrob Agents Chemother 48: 3016–3023.
Wellington EMH et al. 2013. The role of the natural environment in the emergence of antibiotic resistance in Gram-negative bacteria. Lancet Infect Dis 13: 155–165.
Gao X, Shao M, Luo Y, Dong Y, Ouyang F, Dong W, Li J, 2016. Airborne bacterial contaminations in typical Chinese wet market with live poultry trade. Sci Total Environ 572: 681–687.
Li J, Zhou L, Zhang X, Xu C, Dong L, Yao M, 2016. Bioaerosol emissions and detection of airborne antibiotic resistance genes from a wastewater treatment plant. Atmos Environ 124: 404–412.
Echeverria-Palencia CM et al. 2017. Disparate antibiotic resistance gene quantities revealed across 4 major cities in California: a survey in drinking water, air, and soil at 24 public parks. ACS Omega 2: 2255–2263.
Sancheza HM et al. 2016. Antibiotic resistance in airborne bacteria near conventional and organic beef cattle farms in California, USA. Water Air Soil Pollut: 227–280. doi: 10.1007/s11270-016-2979-8.
Rizzo L, Manaia C, Merlin C, Schwartz T, Dagot C, Ploy MC, Michael I, Fatta-Kassinos D, 2013. Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: a review. Sci Total Environ 447: 345–360.
Liu D et al. 2012. Formation and transmission of Staphylococcus aureus (including MRSA) aerosols carrying antibiotic-resistant genes in a poultry farming environment. Sci Total Environ 426: 139–145.
McEachran AD, Blackwell BR, Hanson JD, Wooten KJ, Mayer GD, Cox SB, Smith PN, 2015. Antibiotics, bacteria, and antibiotic resistance genes: aerial transport from cattle feed yards via particulate matter. Environ Health Perspect 123: 337–343.
Hu J, Zhao F, Zhang XX, Li K, Li C, Ye L, Li M, 2018. Metagenomic profiling of ARGs in airborne particulate matters during a severe smog event. Sci Total Environ 615: 1332–1340.
Zhu YG, Johnson TA, Su JQ, Qiao M, Guo GX, Stedtfeld RD, Hashsham SA, Tiedje JM, 2013. Diverse and abundant antibiotic resistance genes in Chinese swine farms. Proc Natl Acad Sci USA 110: 3435–3440.
Zhang M, Zuo J, Yu X, Shi X, Chen L, Li Z, 2017. Quantification of multi-antibiotic resistant opportunistic pathogenic bacteria in bioaerosols in and around a pharmaceutical wastewater treatment plant. J Environ Sci 72: 53–63.
Graham DW, Giesen MJ, Bunce JT, 2018. Strategic approach for prioritising local and regional sanitation interventions for reducing global antibiotic resistance. Water (Switzerland) 11: 27.
Pal C, Bengtsson-Palme J, Kristiansson E, Larsson DGJ, 2016. The structure and diversity of human, animal and environmental resistomes. Microbiome 4: 54.
Gao M, Qiu T, Sun Y, Wang X, 2018. The abundance and diversity of antibiotic resistance genes in the atmospheric environment of composting plants. Environ Int 116: 229–238.
Chapin A, Rule A, Gibson K, Buckley T, Schwab K, 2005. Airborne multidrug-resistant bacteria isolated from a concentrated swine feeding operation. Environ Health Perspect 113: 137–142.
Gibbs SG, Green CF, Tarwater PM, Mota LC, Mena KD, Scarpino PV, 2006. Isolation of antibiotic-resistant bacteria from the air plume downwind of a swine confined or concentrated animal feeding operation. Environ Health Perspect 114: 1032–1037.
Hyvärinen AP, Raatikainen T, Brus D, Komppula M, Panwar TS, Hooda RK, Sharma VP, Lihavainen H, 2011. Effect of the summer monsoon on aerosols at two measurement stations in Northern India–Part 1: PM and BC concentrations. Atmos Chem Phys 11: 8271–8282.
Kumar P, 2011. Census of India, Series 10 Part XII–A District Census Handbook. Kanpur Nagar, Uttar Pradesh: Directorate of Census Operations.
Singh RP, 2001. Effect of wastewater disposal and extent of industrial pollution in and around Kanpur, Uttar Pradesh, India. Bull Eng Geol Environ 60: 31–35.
Murowchick P, Alburty D, 2013. Technical Report: Dry Filter Collection/Wet Elution Aspiration and Recovery Testing. Drexel, MO: AlburtyLab, Inc.
Kodaka H, Mizuochi S, Teramura H, Nirazuka T, 2006. Comparison of the compact dry EC with the most probably number method (AOAC offical method 96624) for enumeration of Escherichia coli and coliform bacteria in raw meats. J AOAC Int 89: 100–114.
Hill V, Narayanan J, Gallen R, Ferdinand K, Cromeans T, Vinjé J, 2015. Development of a nucleic acid extraction procedure for simultaneous recovery of DNA and RNA from diverse microbes in water. Pathogens 4: 335–354.
Chiang C, Liu C, Weng L, Wang N, Liaw G, 2005. Presence of β -lactamase gene TEM-1 DNA sequence in commercial taq DNA polymerase. J Clin Microbiol 43: 530–532.
Guarddon M, Miranda JM, Rodríguez JA, Vázquez BI, Cepeda A, Franco CM, 2011. Real-time polymerase chain reaction for the quantitative detection of tetA and tetB bacterial tetracycline resistance genes in food. Int J Food Microbiol 146: 284–289.
Cavé L, Brothier E, Abrouk D, Bouda PS, Hien E, Nazaret S, 2016. Efficiency and sensitivity of the digital droplet PCR for the quantification of antibiotic resistance genes in soils and organic residues. Appl Microbiol Biotechnol 100: 10597–10608.
Lachmayr KL, Cavanaugh CM, Kerkhof LJ, DiRienzo AG, Ford TE, 2009. Quantifying nonspecific tem β-lactamase (blatem) genes in a wastewater stream. Appl Environ Microbiol 75: 203–211.
Barraud O, Baclet MC, Denis F, Ploy MC, 2010. Quantitative multiplex real-time PCR for detecting class 1, 2 and 3 integrons. J Antimicrob Chemother 65: 1642–1645.
Holloway K, Mathai E, Sorensen T, Gray A, 2008. Community-based surveillance of antimicrobial use and resistance in resource-constrained settings. Anim Genet 39: 561–563.
Stokdyk JP, Firnstahl AD, Spencer SK, Burch TR, Borchardt MA, 2016. Determining the 95% limit of detection for waterborne pathogen analyses from primary concentration to qPCR. Water Res 96: 105–113.
Bivins A, Lowry S, Murphy HM, Borchardt M, Coyte R, Labhasetwar P, Brown J, 2020. Waterborne pathogen monitoring in Jaipur, India reveals potential microbial risks of urban groundwater supply. Npj Clean Water 3: 35.
Cronholm LS, 1980. Potential health hazards from microbial aerosols in densely populated urban regions. Appl Environ Microbiol 39: 6–12.
Farling S, Rogers T, Knee JS, Tilley EA, Brown J, Deshusses MA, 2019. Bioaerosol emissions associated with pit latrine emptying operations. Sci Total Environ 648: 1082–1086.
Salazar D, Ginn O, Brown J, Soria F, Garvizu C, 2020. Assessment of antibiotic resistant coliforms from bioaerosol samples collected above a sewage-polluted river in La Paz, Bolivia. Int J Hyg Environ Health 228: 113494.
Ling AL, Pace NR, Hernandez MT, Lapara TM, 2013. Tetracycline Resistance and Class 1 Integron Genes Associated with Indoor and Outdoor Aerosols. Environmental Science and Technology47: 4046–4052. doi: 10.1021/es400238g.
Heo KJ, Kim HB, Lee BU, 2014. Concentration of environmental fungal and bacterial bioaerosols during the monsoon season. J Aerosol Sci 77: 31–37.
Wagner EG, Lanoix JN, 1958. Excreta Disposal for Rural Areas and Small Communities. Geneva, Switzerland: World Health Organization, Monograph Series, No. 39, 1–184.
Van Boeckel TP, Gandra S, Ashok A, Caudron Q, Grenfell BT, Levin SA, Laxminarayan R, 2014. Global antibiotic consumption 2000 to 2010: an analysis of national pharmaceutical sales data. Lancet Infect Dis 14: 742–750.
Bartley PS, Domitrovic TN, Moretto VT, Santos CS, Ponce-Terashima R, Reis MG, Barbosa LM, Blanton RE, Bonomo RA, Perez F, 2019. Antibiotic resistance in enterobacteriaceae from surface waters in Urban Brazil highlights the risks of poor sanitation. Am J Trop Med Hyg 100: 1369–1377.
Guyomard-Rabenirina S, Dartron C, Falord M, Sadikalay S, Ducat C, Richard V, Breurec S, Gros O, Talarmin A, 2017. Resistance to antimicrobial drugs in different surface waters and wastewaters of Guadeloupe. PLoS One 12: 1–17.
Watkinson AJ, Micalizzi GB, Graham GM, Bates JB, Costanzo SD, 2007. Antibiotic-resistant Escherichia coli in wastewaters, surface waters, and oysters from an urban riverine system. Appl Environ Microbiol 73: 5667–5670.
Lue-Hing C, Zenz DR, Sedita SJ, 1982. Environmental impact of the microbial aerosol emissions from wastewater treatment plants. Water Science and Technology 14: 289–309.
Dueker ME, O’Mullan GD, 2014. Aeration remediation of a polluted waterway increases near-surface coarse and culturable microbial aerosols. Sci Total Environ 478: 184–189.
Pillai SD, Ricke SC, 2002. Bioaerosols from municipal and animal wastes: background and contemporary issues. Can J Microbiol 48: 681–696.
Cao Y, Raith MR, Griffith JF, 2015. Droplet digital PCR for simultaneous quantification of general and human-associated fecal indicators for water quality assessment. Water Res 70: 337–349.
Zhang T, Zhang M, Zhang X, Fang HH, 2009. Tetracycline resistance genes and tetracycline resistant lactose-fermenting enterobacteriaceae in activated sludge of sewage treatment plants. Environ Sci Technol 43: 3455–3460.
Zhang X, Wu B, Zhang Y, Zhang T, Yang L, Fang HHP, Ford T, Cheng S, 2009. Class 1 integronase gene and tetracycline resistance genes tetA and tetC in different water environments of Jiangsu province, China. Ecotoxicology 18: 652–660.