We greatly acknowledge the support of human volunteers who willingly donated their blood samples for this study. We thank Sophie Knudson and Teri Scott for their assistance in manuscript proofing and editing.We are grateful to FIND Specimen Bank for the human biological biobank samples and David Boyle for critical review of this manuscript.
Wongsrichanalai C, Barcus MJ, Muth S, Sutamihardja A, Wernsdorfer WH, 2007. A review of malaria diagnostic tools: microscopy and rapid diagnostic test (RDT). Am J Trop Med Hyg 77: 119–127.
Cheng Q, Gatton ML, Barnwell J, Chiodini P, McCarthy J, Bell D, Cunningham J, 2014. Plasmodium falciparum parasites lacking histidine-rich protein 2 and 3: a review and recommendations for accurate reporting. Malar J 13: 283.
Hurdayal R, Achilonu I, Choveaux D, Coetzer TH, Dean Goldring JP, 2010. Anti-peptide antibodies differentiate between plasmodial lactate dehydrogenases. Peptides 31: 525–532.
Ho MF 2014. Circulating antibodies against Plasmodium falciparum histidine-rich proteins 2 interfere with antigen detection by rapid diagnostic tests. Malar J 13: 480.
Markwalter CF, Jang IK, Burton RA, Domingo GJ, Wright DW, 2017. Biolayer interferometry predicts ELISA performance of monoclonal antibody pairs for Plasmodium falciparum histidine-rich protein 2. Anal Biochem 534: 10–13.
Dalrymple U, Arambepola R, Gething PW, Cameron E, 2018. How long do rapid diagnostic tests remain positive after anti-malarial treatment? Malar J 17: 228.
Ranadive N 2017. Limitations of rapid diagnostic testing in patients with suspected malaria: a diagnostic accuracy evaluation from Swaziland, a low-endemicity country aiming for malaria elimination. Clin Infect Dis 64: 1221–1227.
Rogier E 2017. Bead-based immunoassay allows sub-picogram detection of histidine-rich protein 2 from Plasmodium falciparum and estimates reliability of malaria rapid diagnostic tests. PLoS One 12: e0172139.
Jang IK 2019. Simultaneous quantification of Plasmodium antigens and host factor C-reactive protein in asymptomatic individuals with confirmed malaria by use of a novel multiplex immunoassay. J Clin Microbiol 57: e00948-18.
Das S 2017. Performance of a high-sensitivity rapid diagnostic test for Plasmodium falciparum malaria in asymptomatic individuals from Uganda and Myanmar and naive human challenge infections. Am J Trop Med Hyg 97: 1540–1550.
Gamboa D 2010. A large proportion of P. falciparum isolates in the Amazon region of Peru lack pfhrp2 and pfhrp3: implications for malaria rapid diagnostic tests. PLoS One 5: e8091.
Plucinski MM 2019. Screening for pfhrp2/3-deleted Plasmodium falciparum, non-falciparum, and low-density malaria infections by a multiplex antigen assay. J Infect Dis 219: 437–447.
Plucinski MM, Rogier E, Dimbu PR, Fortes F, Halsey ES, Aidoo M, 2017. Estimating the added utility of highly sensitive histidine-rich protein 2 detection in outpatient clinics in sub-saharan africa. Am J Trop Med Hyg 97: 1159–1162.
Luchavez J, Baker J, Alcantara S, Belizario V Jr., Cheng Q, McCarthy JS, Bell D, 2011. Laboratory demonstration of a prozone-like effect in HRP2-detecting malaria rapid diagnostic tests: implications for clinical management. Malar J 10: 286.
Gillespie SH, Dow C, Raynes JG, Behrens RH, Chiodini PL, McAdam KP, 1991. Measurement of acute phase proteins for assessing severity of Plasmodium falciparum malaria. J Clin Pathol 44: 228–231.
Andrade BB, Reis-Filho A, Souza-Neto SM, Clarencio J, Camargo LM, Barral A, Barral-Netto M, 2010. Severe Plasmodium vivax malaria exhibits marked inflammatory imbalance. Malar J 9: 13.
Iqbal J, Siddique A, Jameel M, Hira PR, 2004. Persistent histidine-rich protein 2, parasite lactate dehydrogenase, and panmalarial antigen reactivity after clearance of Plasmodium falciparum monoinfection. J Clin Microbiol 42: 4237–4241.