Volume 77, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


An agar plate (AP) method has been developed for culturing infectious larvae of the hookworm . The third-stage larvae reared using the AP method displayed similar morphology to those cultured using Baermann or Harada-Mori coproculture techniques. The yield of viable larvae from the AP method (50%) was comparable to that of the Baermann (47%), and both were superior to Harada-Mori (2.1%). Third-stage larvae cultured by the AP method established patent infection in naturally permissive laboratory hosts, although the yield of adult worms was reduced compared with animals infected with L3 obtained by Baermann culture. The AP method is useful for defining growth requirements for hookworm development, as well as characterizing the effects of bacterially expressed compounds on hookworm larvae .


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  1. Schad GA, 1979. Ancylostoma duodenale: maintenance through six generations in helminth-native pups. Exp Parasitol 47 : 246–253. [Google Scholar]
  2. Behnke JM, Paul V, Rajasekariah GR, 1986. The growth and migration of Necator americanus following infection of neonatal hamsters. Trans R Soc Trop Med Hyg 80 : 146–149. [Google Scholar]
  3. Garside P, Behnke JM, 1989. Ancylostoma ceylanicum in the hamster: observations on the host-parasite relationship during primary infection. Parasitology 98 : 283–289. [Google Scholar]
  4. Chiejina SN, 1982. Evaluation of modified Baermann apparatus for the recovery of infective trichostrongylid larvae from herbage samples. J Helminthol 56 : 105–109. [Google Scholar]
  5. Persson L, 1974. A modified baermann apparatus for the recovery of infective nematode larvae from herbage and manure. Zentralbl Veterinarmed B 21 : 483–488. [Google Scholar]
  6. Marchi Blatt J, Cantos GA, 2003. Evaluation of techniques for the diagnosis of Strongyloides stercoralis in human immunodeficiency virus (HIV) positive and HIV negative individuals in the city of Itajai, Brazil. Braz J Infect Dis 7 : 402–408. [Google Scholar]
  7. Navitsky RC, Dreyfuss ML, Shrestha J, Khatry SK, Stoltzfus RJ, Albonico M, 1998. Ancylostoma duodenale is responsible for hookworm infections among pregnant women in the rural plains of Nepal. J Parasitol 84 : 647–651. [Google Scholar]
  8. Bungiro RD Jr, Greene J, Kruglov E, Cappello M, 2001. Mitigation of hookworm disease by immunization with soluble extracts of Ancylostoma ceylanicum. J Infect Dis 183 : 1380–1387. [Google Scholar]
  9. Watson JM, Al-Hafidh R, 1957. A modification of the Baermann funnel technique, and its use in establishing the infection potential of human hookworm-carriers. Ann Trop Med Parasitol 51 : 15–16. [Google Scholar]
  10. Foreyt WJ, 1989. Diagnostic parasitology. Vet Clin North Am Small Anim Pract 19 : 979–1000. [Google Scholar]
  11. Baerman G, 1917. Eine einfache Methode zur Auffindung von Anklostomum-(Nematoden)-Larven in Erdproben. Tijdschr Diergeneeskd 57 : 131–137. [Google Scholar]
  12. Harada Y, Mori O, 1955. A new method for culturing hookworm. Yonago Acta Med 1 : 177–179. [Google Scholar]
  13. Grant WN, Stasiuk S, Newton-Howes J, Ralston M, Bisset SA, Heath DD, Shoemaker CB, 2006. Parastrongyloides trichosuri, a nematode parasite of mammals that is uniquely suited to genetic analysis. Int J Parasitol 36 : 453–466. [Google Scholar]
  14. Brenner S, 1974. The genetics of Caenorhabditis elegans. Genetics 77 : 71–94. [Google Scholar]
  15. Couillault C, Ewbank JJ, 2002. Diverse bacteria are pathogens of Caenorhabditis elegans. Infect Immun 70 : 4705–4707. [Google Scholar]
  16. Kotze AC, Coleman GT, Mai A, McCarthy JS, 2005. Field evaluation of anthelmintic drug sensitivity using in vitro egg hatch and larval motility assays with Necator americanus recovered from human clinical isolates. Int J Parasitol 35 : 445–453. [Google Scholar]
  17. Sulston J, Hodgkin J, 1988. Methods. Wood WB, ed. The Nematode Caenorhabditis elegans. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 587–606.
  18. Jongwutiwes S, Charoenkorn M, Sitthichareonchai P, Akaraborvorn P, Putaporntip C, 1999. Increased sensitivity of routine laboratory detection of Strongyloides stercoralis and hookworm by agar-plate culture. Trans R Soc Trop Med Hyg 93 : 398–400. [Google Scholar]
  19. Kotze AC, Clifford S, O’Grady J, Behnke JM, McCarthy JS, 2004. An in vitro larval motility assay to determine anthelmintic sensitivity for human hookworm and Strongyloides species. Am J Trop Med Hyg 71 : 608–616. [Google Scholar]
  20. Yoshida Y, Okamoto K, Chiu JK, 1968. Ancylostoma ceylanicum infection in dogs, cats, and man in Taiwan. Am J Trop Med Hyg 17 : 378–381. [Google Scholar]
  21. Chowdhury AB, Schad GA, 1972. Ancylostoma ceylanicum: a parasite of man in Calcutta and environs. Am J Trop Med Hyg 21 : 300–301. [Google Scholar]
  22. Carroll SM, Grove DI, 1986. Experimental infection of humans with Ancylostoma ceylanicum: clinical, parasitological, haematological and immunological findings. Trop Geogr Med 38 : 38–45. [Google Scholar]
  23. Held MR, Bungiro RD, Harrison LM, Hamza I, Cappello M, 2006. Dietary iron content mediates hookworm pathogenesis in vivo. Infect Immun 74 : 289–295. [Google Scholar]
  24. Bungiro R, Cappello M, 2004. Hookworm infection: new developments and prospects for control. Curr Opin Infect Dis 17 : 421–426. [Google Scholar]
  25. Jones BF, Cappello M, 2004. Hookworm infection: molecular mechanisms of disease and targets for control. Drug Disc Today 1 : 217–222. [Google Scholar]

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  • Received : 07 May 2007
  • Accepted : 15 Aug 2007

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