1921
Volume 99, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract.

The aim of this study was to demonstrate the presence of eggs in beetles collected from sources within the natural environment through molecular techniques. Fifty-four pools of beetles were collected in three villages in Piura, Peru. DNA was extracted using the FastDNA spin kit for soil. Molecular identification of species was then performed through partial amplification of the mitochondrial cytochrome C oxidase subunit I gene. Finally, positive samples were sequenced to determine the tapeworm species. Seven positive samples were obtained through polymerase chain reaction amplification. Sequencing confirmed that two samples were from and three samples were from . The other two samples could not be specifically identified. Our findings demonstrate that dung beetles ingest and eggs under natural conditions and suggest that beetles may play a role in the dynamics of transmission of these cestodes.

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  • Received : 25 Apr 2018
  • Accepted : 13 Jul 2018
  • Published online : 10 Sep 2018

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