1921
Volume 94, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

Opisthorchiasis is a neglected tropical disease, yet it is of considerable public health importance in Southeast Asia given the predilection for chronically infected persons to develop cholangiocarcinoma. We evaluated a handheld microscope for the diagnosis of in a community-based setting in Lao People's Democratic Republic in comparison with conventional light microscopy. In stool samples collected from 104 individuals, handheld microscopy revealed a sensitivity of 70.6% and a specificity of 89.5% for infection. Pearson's correlation for quantitative fecal egg counts between the two devices was 0.98 (95% confidence interval: 0.98–0.99). With small adjustments to further increase diagnostic sensitivity, a handheld microscope may become a helpful tool to screen for and other helminth infections in public health settings.

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References

  1. Petti CA, Polage CR, Quinn TC, Ronald AR, Sande MA, , 2006. Laboratory medicine in Africa: a barrier to effective health care. Clin Infect Dis 42: 377382.[Crossref] [Google Scholar]
  2. Howitt P, Darzi A, Yang G-Z, Ashrafian H, Atun R, Barlow J, Blakemore A, Bull AMJ, Car J, Conteh L, Cooke GS, Ford N, Gregson SAJ, Kerr K, King D, Kulendran M, Malkin RA, Majeed A, Matlin S, Merrifield R, Penfold HA, Reid SD, Smith PC, Stevens MM, Templeton MR, Vincent C, Wilson E, , 2012. Technologies for global health. Lancet 380: 507535.[Crossref] [Google Scholar]
  3. Yansouni CP, Bottieau E, Lutumba P, Winkler AS, Lynen L, Büscher P, Jacobs J, Gillet P, Lejon V, Alirol E, Polman K, Utzinger J, Miles MA, Peeling RW, Muyembe JJ, Chappuis F, Boelaert M, , 2013. Rapid diagnostic tests for neurological infections in central Africa. Lancet Infect Dis 13: 546558.[Crossref] [Google Scholar]
  4. Utzinger J, Becker SL, van Lieshout L, van Dam GJ, Knopp S, , 2015. New diagnostic tools in schistosomiasis. Clin Microbiol Infect 21: 529542.[Crossref] [Google Scholar]
  5. Stothard JR, Kabatereine NB, Tukahebwa EM, Kazibwe F, Mathieson W, Webster JP, Fenwick A, , 2005. Field evaluation of the Meade Readiview handheld microscope for diagnosis of intestinal schistosomiasis in Ugandan school children. Am J Trop Med Hyg 73: 949955. [Google Scholar]
  6. Bogoch II, Andrews JR, Speich B, Utzinger J, Ame SM, Ali SM, Keiser J, , 2013. Mobile phone microscopy for the diagnosis of soil-transmitted helminth infections: a proof-of-concept study. Am J Trop Med Hyg 88: 626629.[Crossref] [Google Scholar]
  7. Bogoch II, Coulibaly JT, Andrews JR, Speich B, Keiser J, Stothard JR, N'Goran EK, Utzinger J, , 2014. Evaluation of portable microscopic devices for the diagnosis of Schistosoma and soil-transmitted helminth infection. Parasitology 141: 18111818.[Crossref] [Google Scholar]
  8. Bogoch II, Andrews JR, Speich B, Ame SM, Ali SM, Stothard JR, Utzinger J, Keiser J, , 2014. Quantitative evaluation of a handheld light microscope for field diagnosis of soil-transmitted helminth infection. Am J Trop Med Hyg 91: 11381141.[Crossref] [Google Scholar]
  9. Stothard JR, Nabatte B, Sousa-Figueiredo JC, Kabatereine NB, , 2014. Towards malaria microscopy at the point-of-contact: an assessment of the diagnostic performance of the Newton Nm1 microscope in Uganda. Parasitology 141: 18191825.[Crossref] [Google Scholar]
  10. Ephraim RKD, Duah E, Cybulski JS, Prakash M, D'Ambrosio MV, Fletcher DA, Keiser J, Andrews JR, Bogoch II, , 2015. Diagnosis of Schistosoma haematobium infection with a mobile phone-mounted foldscope and a reversed-lens CellScope in Ghana. Am J Trop Med Hyg 92: 12531256.[Crossref] [Google Scholar]
  11. Keiser J, Utzinger J, , 2009. Food-borne trematodiases. Clin Microbiol Rev 22: 466483.[Crossref] [Google Scholar]
  12. Sripa B, Bethony JM, Sithithaworn P, Kaewkes S, Mairiang E, Loukas A, Mulvenna J, Laha T, Hotez PJ, Brindley PJ, , 2011. Opisthorchiasis and Opisthorchis-associated cholangiocarcinoma in Thailand and Laos. Acta Trop 120 (Suppl 1): S158S168.[Crossref] [Google Scholar]
  13. Fürst T, Sayasone S, Odermatt P, Keiser J, Utzinger J, , 2012. Manifestation, diagnosis, and management of foodborne trematodiasis. BMJ 344: e4093.[Crossref] [Google Scholar]
  14. Sripa B, Brindley PJ, Mulvenna J, Laha T, Smout MJ, Mairiang E, Bethony JM, Loukas A, , 2012. The tumorigenic liver fluke Opisthorchis viverrini—multiple pathways to cancer. Trends Parasitol 28: 395407.[Crossref] [Google Scholar]
  15. Katz N, Chaves A, Pellegrino J, , 1972. A simple device for quantitative stool thick-smear technique in schistosomiasis mansoni. Rev Inst Med Trop São Paulo 14: 397400. [Google Scholar]
  16. Speich B, Ali SM, Ame SM, Albonico M, Utzinger J, Keiser J, , 2015. Quality control in the diagnosis of Trichuris trichiura and Ascaris lumbricoides using the Kato-Katz technique: experience from three randomised controlled trials. Parasit Vectors 8: 82.[Crossref] [Google Scholar]
  17. Switz NA, D'Ambrosio MV, Fletcher DA, , 2014. Low-cost mobile phone microscopy with a reversed mobile phone camera lens. PLoS One 22: e95330.[Crossref] [Google Scholar]
  18. Maleewong W, Intapan P, Wongwajana S, Sitthithaworn P, Pipitgool V, Wongkham C, Daenseegaew W, , 1992. Prevalence and intensity of Opisthorchis viverrini in rural community near the Mekong River on the Thai-Laos border in northeast Thailand. J Med Assoc Thai 75: 231235. [Google Scholar]
  19. WHO, 1994. Bench Aids for the Diagnosis of Intestinal Parasites. Available at: http://apps.who.int/iris/bitstream/10665/37323/1/9789241544764_eng.pdf. Accessed June 23, 2015. [Google Scholar]
  20. de Vlas SJ, Gryseels B, , 1992. Underestimation of Schistosoma mansoni prevalences. Parasitol Today 8: 274277.[Crossref] [Google Scholar]
  21. Knopp S, Mgeni AF, Khamis IS, Steinmann P, Stothard JR, Rollinson D, Marti H, Utzinger J, , 2008. Diagnosis of soil-transmitted helminths in the era of preventive chemotherapy: effect of multiple stool sampling and use of different diagnostic techniques. PLoS Negl Trop Dis 2: e331.[Crossref] [Google Scholar]
  22. Sayasone S, Utzinger J, Akkhavong K, Odermatt P, , 2015. Repeated stool sampling and use of multiple techniques enhance the sensitivity of helminth diagnosis: a cross-sectional survey in southern Lao People's Democratic Republic. Acta Trop 141: 315321.[Crossref] [Google Scholar]
  23. Sithithaworn P, Haswell-Elkins M, , 2003. Epidemiology of Opisthorchis viverrini . Acta Trop 88: 187194.[Crossref] [Google Scholar]
  24. Sayasone S, Odermatt P, Phoumindr N, Vongsaravane X, Sensombath V, Phetsouvanh R, Choulamany X, Strobel M, , 2007. Epidemiology of Opisthorchis viverrini in a rural district of southern Lao PDR. Trans R Soc Trop Med Hyg 101: 4047.[Crossref] [Google Scholar]
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  • Received : 13 Jul 2015
  • Accepted : 25 Sep 2015
  • Published online : 06 Jan 2016

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