Volume 89, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The presence of species in fleas collected from spp. (, , and ) in Khon Kaen Province, Thailand was investigated. One hundred ninety-three fleas obtained from 62 rats, were screened by polymerase chain reaction using primers specific for the 16S–23S intergenic spacer region, and the presence of DNA was confirmed by using the citrate synthase gene. DNA was detected in 59.1% (114 of 193) of fleas examined. Sequencing demonstrated the presence of spp. similar to , , , and in the samples tested with a cutoff for sequence similarity ≥ 96% and 4 clustered together with the closest match with (95.5% identity). If proves to be a competent vector of these species, our results suggest that humans and animals residing in this area may be at risk for infection by several zoonotic species.


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  • Received : 09 Aug 2012
  • Accepted : 01 Feb 2013
  • Published online : 04 Sep 2013

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