Observations Upon the Cultivation of Endamoeba Histolytica

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  • Department of Preventive Medicine and Clinical Pathology, Army Medical School, Washington, D. C.

In a previous communication (1) the writer described the cultivation of Endamoeba histolytica, the cause of amoebic dysentery, in a medium composed of seven parts of Locke solution and one part of inactivated human, horse, or rabbit blood serum. In this medium the organism remained alive for as long as eleven days and daily transfers were successful for a period of over three months. Since the publication of that communication this strain of Endamoeba histolytica, proved pathogenic to kittens, has been maintained in the same medium for a period of five months, during which time over 140 transfers have been made, at daily intervals. These observations demonstrate that coagulated egg or blood are not necessary as a part of the food supply of this species and that a solid sub-stratum is equally unnecessary for the successful cultivation of the parasite.