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I came to this meeting to support the work of Drs. Hussey and Brown, but now I find that I am going to change my talk and violently oppose Hussey's and Brown's conclusions as to the value of the complement fixation test for the diagnosis of intestinal amebiasis. We have been conducting this test since about 1942, and have run over 13,000 tests. I want to point out that there probably is a little discrepancy in the technic and the antigens employed which is really responsible for the difference in the results between the two laboratories. In the first place, they are using the three 50 per cent units of complement for the titration and a more concentrated antigen as prescribed by Kent and Rein (1946). Now the original work instituted in the Army Medical Center was done at the instigation of General Callender. He was interested in amebiasis and began all the work at Walter Reed.
National Institutes of Health, U. S. Public Health Service, Bethesda, Md.