Acetic-Alcohol Fixation for Parasitic Amoebae and for Tissues

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  • Laboratory Service, Walter Reed Hospital, Washington, D.C.


The use of Carnoy's acetic-alcohol as a fixing agent affords a rapid and certain method of fixing the parasitic amoebae for staining. This agent, consisting of absolute alcohol 6 parts, chloroform 3 parts, and glacial acetic acid 1 part, is used cold and insures quick killing, rapid fixation and fidelity of tissue elements when followed by hematoxylin stains. Fresh smears are completely fixed in ten to fifteen minutes and pieces of tissue in three hours. The fixative is followed by washing in absolute alcohol (one change) following which the tissue or smear is placed in absolute alcohol.