Laboratory Diagnosis of Cholera

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Summary and Conclusions

  1. 1.A simple method for the laboratory diagnosis of cholera is presented: (a) Peptone water is inoculated with fresh stool and incubated 8 hours, (b) Alkaline nutrient agar plates are streaked and incubated overnight, (c) Transparent colonies are tested for agglutination with anti-cholera O serum. (d) Agglutinable vibrio are tested for hemolysis using 5 per cent sheep or goat cells.
  2. 2.V. cholerae are present in the intestinal tract only in the first few days of illness, therefore sulfonamides or other bactericidal agents are effective only if given early in the course of the disease.
  3. 3.The value of sulfonamides in cholera carriers cannot be definitely evaluated at this time.
  4. 4.Of 3,000 contacts, 61 became contact carriers. Two of these developed the disease. The number of clinical cases developing from contact carriers is very small.

Author Notes

The College of Physicians and Surgeons, Columbia University, New York, New York.

The Schering International Research Institute, Bloomfield, New Jersey and Rio de Janeiro, Brazil.