In 1933 (1) there was described the technique by which we had developed a dependable routine for the employment of naturally induced malaria in the therapy of paresis. In the course of time our procedures have undergone certain modifications directed toward simplification as well as increased reliability, which it appears desirable to note. Discussion of the modifications will follow the topical order of the paper cited.
For several years our anopheline requirements have been adequately met by the insectaries in which propagating colonies are maintained, and the employment of wild specimens has been abandoned. Our insectary technique has been fully described (2). The colonies of A. quadrimaculatus and A. punctipennis afford an adequate supply of large, vigorous, uninfected females at all seasons of the year. Our routine work is carried out exclusively with the former species, owing to its greater susceptibility to the different species of parasites.