The Cultivation of Trichomonas Hominis

Mary Jane HogueDepartment of Medical Zoology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland

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There are many different species of Trichomonas, three of which have been described as parasitic in man. These are Trichomonas buccalis, Goodey and Wellings; Trichomonas hominis, Davaine; and Trichomonas vaginalis, Donné. It is even possible that these three species are the same but this has not yet been proved.

In 1915 Lynch cultured Trichomonas hominis from the intestine in neutral and acid bouillon and kept it alive for three days at 30°C. By transferring to new media he was able to carry the culture through five or six generations.

Ohira and Noguchi (1917) report the cultivation of the Trichomonas from the human mouth calling it Tetratrichomonas hominis. According to Kofoid's (1920) recent review of the nomenclature of the flagellates, Ohira and Noguchi were working with Trichomonas buccalis. They cultured it on a mixture of ascitic fluid and Ringer's solution, using equal portions and found that it gave much better results than Lynch's bouillon medium.