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Concordance between a New Rapid Point-Of-Care Assay and Standard ELISA in the Detection of Cysticercosis Antigens in Urine
Luz M. Toribio
Luz M. Toribio
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
Infection and Immunity Institute, St. George’s University of London, London, United Kingdom;
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Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
Infection and Immunity Institute, St. George’s University of London, London, United Kingdom;
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Alessandra Vásquez
Alessandra Vásquez
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
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Yesenia Castillo
Yesenia Castillo
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
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S. Mathof Salas
S. Mathof Salas
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
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Erika Perez
Erika Perez
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
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Javier A. Bustos
Javier A. Bustos
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
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Seth E. O’Neal
Seth E. O’Neal
School of Public Health, Oregon Health & Sciences University-Portland State University, Portland, Oregon;
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Hector H. Garcia
for The Cysticercosis Working Group in Peru
Hector H. Garcia
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
Asociacion Benefica PRISMA, Lima, Peru;
Department of International Health, Johns Hopkins School of Public Health, Baltimore, Maryland
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Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
Asociacion Benefica PRISMA, Lima, Peru;
Department of International Health, Johns Hopkins School of Public Health, Baltimore, Maryland
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Neurocysticercosis is a parasitic disease of major public health importance. Definitive diagnosis requires neuroimaging, which is typically unavailable in rural impoverished regions of endemicity. Screening immunoassays can support diagnosis in this setting by identifying individuals most likely to have severe forms of disease for referral to imaging. Urine sampling is convenient, painless, and generally well accepted. We developed a rapid point-of-care (POC) assay to detect urinary antigens and assessed concordance with a standard antigen ELISA (Ag-ELISA), both using monoclonal antibodies TsW8/TsW5. From 28,145 stored community samples with Ag-ELISA results, we selected 843 for comparison, 281 each from nonreactive (ratio <1), reactive-below-cutoff (ratio 1:3), and positive (ratio ≥3) samples. Overall agreement was 73.6%, with strong agreement observed in the nonreactive (280/281, 99.6%) and positive (255/281, 90.8%) groups. This affordable noninvasive POC test can be applied to identify individuals in the community most at risk of developing severe disease.
Author Notes
Financial support: H. H. Garcia received funding from the
Disclosure: Both the parent study and the current study were approved by the institutional review board of the Universidad Peruana Cayetano Heredia (#161-14-23).
Current contact information: Luz M. Toribio, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru, Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru, and Infection and Immunity Institute, St. George’s University of London, London, United Kingdom, E-mail: ltoribio@sgul.ac.uk. Alessandra Vásquez and Yesenia Castillo, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru, E-mails: alessandra.vasquez@upch.pe and yesenia.castillo.b@upch.pe. S. Mathof Salas and Erika Perez, Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru, E-mails: selene.salas@upch.pe and perez.paredes.e@gmail.com. Javier A. Bustos, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru, and Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru, E-mail: javier.bustos.p@upch.pe. Seth E. O’Neal, School of Public Health, Oregon Health & Sciences University-Portland State University, Portland, OR, E-mail: natseth7@gmail.com. Hector H. Garcia, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru, Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru, Asociacion Benefica PRISMA, Lima, Peru, and Department of International Health, Johns Hopkins School of Public Health, Baltimore, MD, E-mail: hgarcia@jhsph.edu.
| Past two years | Past Year | Past 30 Days | |
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| PDF Downloads | 4 | 4 | 1 |