Rapid Detection of Severe Fever with Thrombocytopenia Syndrome Virus in the Acute Phase of Infection by Direct Real-Time Reverse Transcription without RNA Extraction

Takehiro Hashimoto Infection Control Center, Oita University Hospital, Oita, Japan;
Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;

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Takaaki Yahiro Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;
Department of Advanced Medical Sciences, Oita University Faculty of Medicine, Oita, Japan;
Research Center for Global and Local Infectious Diseases, Oita, Japan;

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Kazuma Ono Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;

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Ryuichi Takenaka Department of Emergency Medicine, Oita University Faculty of Medicine, Oita, Japan

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Catalino Demetria Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;

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Sakirul Khan Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;

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Kazunori Kimitsuki Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;

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Ryuzo Abe Department of Emergency Medicine, Oita University Faculty of Medicine, Oita, Japan

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Kazufumi Hiramatsu Infection Control Center, Oita University Hospital, Oita, Japan;
Research Center for Global and Local Infectious Diseases, Oita, Japan;

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Akira Nishizono Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;
Research Center for Global and Local Infectious Diseases, Oita, Japan;

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No specific treatment has been developed for severe fever with thrombocytopenia syndrome (SFTS). However, the prognosis can improve with early plasma exchange. Therefore, rapid and accurate detection of SFTS virus is important for diagnosis and prognosis. Direct real-time reverse transcription polymerase chain reaction (RT-PCR) testing is easier and more time-efficient than conventional real-time RT-PCR. Our study compared direct real-time RT-PCR efficiency without the RNA extraction and purification of conventional real-time RT-PCR. Samples were collected from 18 patients with SFTS and five without SFTS. A strong correlation (r = 0.774, 95% CI: 0.652–0.857, P <0.01) was found between conventional and direct real-time RT-PCR assays. Direct real-time RT-PCR showed 84.4% sensitivity and 92.0% specificity for viral detection. Direct real-time RT-PCR is an effective diagnostic tool for patients with acute phase SFTS, but further optimization is required for viral detection.

Author Notes

Financial support: This work is supported in part by the grants from the Japan Society for the Promotion of Science to Takehiro Hashimoto (Grant no. 23K11532).

Authors’ addresses: Takehiro Hashimoto, Infection Control Center, Oita University Hospital, Oita, Japan, and Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan, E-mail: hashimo2013@oita-u.ac.jp. Takaaki Yahiro, Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan, Department of Advanced Medical Sciences, Oita University Faculty of Medicine, Oita, Japan, and Research Center for Global and Local Infectious Diseases, Oita, Japan, E-mail: takaaki-816@oita-u.ac.jp. Kazuma Ono, Catalino Demetria, Sakirul Khan, and Kazunori Kimitsuki, Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan, E-mails: m1841018@oita-u.ac.jp, c_demetria@yahoo.com.ph, and sakirul@oita-u.ac.jp, k-kimitsuki@oita-u.ac.jp. Ryuichi Takenaka and Ryuzo Abe, Department of Emergency Medicine, Oita University Faculty of Medicine, Oita, Japan, E-mails: ryuichi@oita-u.ac.jp and ryuzoabe@oita-u.ac.jp. Kazufumi Hiramatsu, Infection Control Center, Oita University Hospital, Oita, Japan, and Research Center for Global and Local Infectious Diseases, Oita, Japan, E-mail: hiramats@oita-u.ac.jp. Akira Nishizono, Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan and Research Center for Global and Local Infectious Diseases, Oita, Japan, E-mail: a24zono@oita-u.ac.jp.

Address correspondence to Takehiro Hashimoto, Infection Control Center, Oita University Hospital, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan. E-mail: hashimo2013@oita-u.ac.jp
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