Monitoring and Predicting Net Longevity by Measuring Surface Levels of Insecticide: Implementing a Faster, Cost Effective, Nondestructive, and Field-Ready Alternative to the World Health Organization Cone Test Bioassay

David A. Calle Medical Entomology Group, School of Medicine, Universidad de Antioquia, Medellin, Antioquia, Colombia;

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Guillermo L. Rúa-Uribe Medical Entomology Group, School of Medicine, Universidad de Antioquia, Medellin, Antioquia, Colombia;

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Lisardo Osorio Environmental Health Research Group, National School of Public Health, Universidad de Antioquia, Medellin, Antioquia, Colombia;

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Juan Gabriel Piñeros-Jiménez Environmental Health Research Group, National School of Public Health, Universidad de Antioquia, Medellin, Antioquia, Colombia;

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Isabel Swamidoss Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia

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Lucrecia Vizcaino Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia

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Raymond Beach Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia

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Michael D. Green Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia

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DOI:
https://doi.org/10.4269/ajtmh.18-0250
Volume/Issue:
Volume 99: Issue 4
Page(s):
1003–1005
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An important component of malaria control programs is the ability to assess the effectiveness of the insecticide in insecticide-treated nets (ITNs) during normal usage. The standard technique to measure insecticidal activity is the World Health Organization (WHO) cone test, which in many circumstances, may be difficult to implement. We have evaluated an alternative technique, the colorimetric field test (CFT) on a group of 24-month-old Permanet® 2.0 (Vestergaard-Frandsen, Denmark) nets collected in Colombia. The CFT, which measures surface levels (SL) of deltamethrin is compared with standard high-performance liquid chromatography (HPLC) and the WHO cone test. Effective concentrations of deltamethrin for 80% mortality (EC80) were determined from the CFT and HPLC results. Distribution of insecticide SL after 24 months of use reveal that sampling of the midsection best represents the condition of the entire net. We conclude that the CFT is a practical alternative to the WHO cone test for assessing ITN efficacy.

Author Notes

Address correspondence to Michael D. Green, Division of Parasitic Diseases and Malaria, U.S. Centers for Disease Control and Prevention, 1600 Clifton Rd., N.E. Mailstop G49, Atlanta, GA 30680. E-mail: mgreen@cdc.gov

Authors’ addresses: David A. Calle and Guillermo L. Rúa-Uribe, Medical Entomology Group, School of Medicine, Universidad de Antioquia, Medellin, Colombia, E-mails: dcalle.entomo@gmail.com and glruauribe@gmail.com. Lisardo Osorio and Juan Gabriel Piñeros-Jiménez, Environmental Health Research Group, National School of Public Health, Universidad de Antioquia, Medellin, Colombia, E-mails: lisardoq@gmail.com and juan.pineros@udea.edu.co. Isabel Swamidoss, Lucrecia Vizcaino, Raymond Beach, and Michael D. Green, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA, E-mails: iswamidoss@cdc.gov, rvizcainocabarrus@cdc.gov, rbeach@cdc.gov, and mgreen@cdc.gov.

Copyright:
© The American Society of Tropical Medicine and Hygiene 2018
Received:
23 Mar 2018
|
Accepted:
11 May 2018
|
Published Online:
20 Aug 2018
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Online ISSN:
1476-1645
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