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-
1.
Monath TP, Vasconcelos PF, 2015. Yellow fever. J Clin Virol 64: 160–173.
-
2.
Dexheimer Paploski IA et al. 2017. Epizootic outbreak of yellow fever virus and risk for human disease in Salvador, Brazil. Ann Intern Med 168: 301–302.
-
3.
World Health Organization, 2017. Yellow Fever Outbreak Angola, Democratic Republic of the Congo and Uganda 2016–2017. Geneva, Switzerland: World Health Organization. Available at: http://www.who.int/emergencies/yellow-fever/en/. Accessed January 8, 2018.
-
4.
Domingo C, Patel P, Yillah J, Weidmann M, Méndez JA, Nakouné ER, Niedrig M, 2012. Advanced yellow fever virus genome detection in point-of-care facilities and reference laboratories. J Clin Microbiol 50: 4054–4060.
-
5.
World Health Organization, 2016. Yellow Fever. Geneva, Switzerland: World Health Organization. Available at: http://www.who.int/mediacentre/factsheets/fs100/en/. Accessed April 4, 2017.
-
6.
Domingo C, Escadafal C, Rumer L, Mendez JA, Garcia P, Sall AA, Teichmann A, Donoso-Mantke O, Niedrig M, 2012. First international external quality assessment study on molecular and serological methods for yellow fever diagnosis. PLoS One 7: e36291.
-
7.
Pan American Health Organization, 2017. Laboratory Diagnosis of Yellow Fever Virus Infection. Washington, DC: Pan American Health Organization.
-
8.
World Health Organization, 2009. Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control. Geneva, Switzerland: WHO Press.
-
9.
Bae HG, Nitsche A, Teichmann A, Biel SS, Niedrig M, 2003. Detection of yellow fever virus: a comparison of quantitative real-time PCR and plaque assay. J Virol Methods 110: 185–191.
-
10.
Nunes MR, Palacios G, Nunes KN, Casseb SM, Martins LC, Quaresma JA, Savji N, Lipkin WI, Vasconcelos PF, 2011. Evaluation of two molecular methods for the detection of yellow fever virus genome. J Virol Methods 174: 29–34.
-
11.
Reusken CBEM, Knoester M, GeurtsvanKessel C, Koopmans M, Knapen DG, Bierman WFW, Pas S, 2017. Urine as sample type for molecular diagnosis of natural yellow fever virus infections. J Clin Microbiol 55: 3294–3296.
-
12.
Waggoner JJ et al. 2013. Development of an internally controlled real-time reverse transcriptase PCR assay for pan-dengue virus detection and comparison of four molecular dengue virus detection assays. J Clin Microbiol 51: 2172–2181.
-
13.
Waggoner JJ et al. 2014. Multiplex nucleic acid amplification test for diagnosis of dengue fever, malaria, and leptospirosis. J Clin Microbiol 52: 2011–2018.
-
14.
Waggoner JJ, Gresh L, Mohamed-Hadley A, Ballesteros G, Davila MJ, Tellez Y, Sahoo MK, Balmaseda A, Harris E, Pinsky BA, 2016. Single-reaction multiplex reverse transcription PCR for detection of zika, chikungunya, and dengue viruses. Emerg Infect Dis 22: 1295–1297.
-
15.
Burd EM, 2010. Validation of laboratory-developed molecular assays for infectious diseases. Clin Microbiol Rev 23: 550–576.
-
16.
Kuan G, Gordon A, Aviles W, Ortega O, Hammond SN, Elizondo D, Nunez A, Coloma J, Balmaseda A, Harris E, 2009. The Nicaraguan pediatric dengue cohort study: study design, methods, use of information technology, and extension to other infectious diseases. Am J Epidemiol 170: 120–129.
-
17.
Waggoner JJ et al. 2013. Comparison of the FDA-approved CDC DENV-1–4 real-time reverse transcription-PCR with a laboratory-developed assay for dengue virus detection and serotyping. J Clin Microbiol 51: 3418–3420.
-
18.
Colebunders R et al. 2002. A Belgian traveler who acquired yellow fever in the Gambia. Clin Infect Dis 35: e113–e116.
-
19.
Shearer FM et al. 2017. Global yellow fever vaccination coverage from 1970 to 2016: an adjusted retrospective analysis. Lancet Infect Dis 17: 1209–1217.
-
20.
Pan American Health Organization/World Health Organization, 2017. Epidemiological Update: Yellow Fever. Washington, DC: PAHO/WHO.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 1793 | 1501 | 99 |
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| PDF Downloads | 210 | 27 | 6 |
Internally Controlled, Multiplex Real-Time Reverse Transcription PCR for Dengue Virus and Yellow Fever Virus Detection
Alejandra Rojas
Alejandra Rojas
Departamento de Producción, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Asunción, Paraguay;
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Cheikh T. Diagne
Cheikh T. Diagne
Institut Pasteur de Dakar, Dakar, Sénégal;
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Victoria D. Stittleburg
Victoria D. Stittleburg
Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia;
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Alisha Mohamed-Hadley
Alisha Mohamed-Hadley
Department of Pathology, Stanford University School of Medicine, Stanford, California;
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Yvalena Arévalo de Guillén
Yvalena Arévalo de Guillén
Departamento de Producción, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Asunción, Paraguay;
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Angel Balmaseda
Angel Balmaseda
Laboratorio Nacional de Virología, Centro Nacional de Diagnóstico y Referencia, Ministry of Health, Managua, Nicaragua;
Sustainable Sciences Institute, Managua, Nicaragua;
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Sustainable Sciences Institute, Managua, Nicaragua;
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Oumar Faye
Oumar Faye
Institut Pasteur de Dakar, Dakar, Sénégal;
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Ousmane Faye
Ousmane Faye
Institut Pasteur de Dakar, Dakar, Sénégal;
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Amadou A. Sall
Amadou A. Sall
Institut Pasteur de Dakar, Dakar, Sénégal;
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Eva Harris
Eva Harris
Sustainable Sciences Institute, Managua, Nicaragua;
Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California;
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Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California;
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Benjamin A. Pinsky
Benjamin A. Pinsky
Department of Pathology, Stanford University School of Medicine, Stanford, California;
Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California;
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Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California;
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Jesse J. Waggoner
Jesse J. Waggoner
Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia;
Department of Global Health, Rollins School of Public Health, Atlanta, Georgia
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Department of Global Health, Rollins School of Public Health, Atlanta, Georgia
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The differential diagnosis of dengue virus (DENV) and yellow fever virus (YFV) infections in endemic areas is complicated by nonspecific early clinical manifestations. In this study, we describe an internally controlled, multiplex real-time reverse transcription polymerase chain reaction (rRT-PCR) for the detection of DENV and YFV. The DENV–YFV assay demonstrated specific detection and had a dynamic range of 2.0–8.0 log10 copies/μL of eluate for each DENV serotype and YFV. Clinical performance was similar to a published pan-DENV assay: 48/48 acute-phase samples from dengue cases were detected in both assays. For YFV detection, mock samples were prepared with nine geographically diverse YFV isolates over a range of concentrations. The DENV–YFV assay detected 62/65 replicates, whereas 54/65 were detected using a reference YFV rRT-PCR. Given the reemergence of DENV and YFV in areas around the world, the DENV–YFV assay should be a useful tool to narrow the differential diagnosis and provide early case detection.
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Author Notes
Financial support: The research was supported by the National Institutes of Health (NIH) grant K08AI110528 (J. J. W., salary support) and a Robert E. Shope International Fellowship in Infectious Diseases (J. J. W.) distributed by the American Society of Tropical Medicine and Hygiene. Research was also supported by a fellowship from the Consejo Nacional de Ciencia y Tecnología (CONACYT) of Paraguay, awarded as part of the Programa de Vinculación de Científicos y Tecnólogos, PVCT 16-66 (A. R.).
Ethical approval: Research specifically performed for this study involved archived, de-identified patient samples collected as part of routine care or IRB-approved research. As such, this work was considered exempt from review by the Institutional Review Board at Emory University. The Pediatric Dengue Cohort Study, from which dengue samples were obtained, was reviewed and approved by Institutional Review Boards of the Nicaraguan Ministry of Health and the University of California, Berkeley. Parents or legal guardians of all subjects provided written informed consent and subjects ≥ 6 years old provided assent. Serum samples from patients with yellow fever were collected during the outbreak in Angola (2016-2017). These have been de-identified, archived, and approved for future research by the Institut Pasteur de Dakar.
Authors’ addresses: Alejandra Rojas and Yvalena Arévalo de Guillén, Instituto de Investigaciones en Ciencias de la Salud, Producción, Asunción, Central, Paraguay, E-mails: alerojaspy@gmail.com and ivalenaguillen@yahoo.com. Cheikh T. Diagne and Oumar Faye, Department of Virology, Institut Pasteur de Dakar, Dakar, Sénégal, E-mails: cheikhtidiane.diagne@pasteur.sn and oumar.faye@pasteur.sn. Victoria D. Stittleburg, Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, E-mail: victoria.d.simmons@emory.edu. Alisha Mohamed-Hadley, Department of Pathology, Stanford University School of Medicine, Stanford, CA, E-mail: alisha.mohamedhadley@gmail.com. Angel Balmaseda, Departamento de Virología, Centro Nacional de Diagnóstico y Referencia, Ministerio de Salud, Managua, Nicaragua, E-mail: abalmaseda@minsa.gob.ni. Ousmane Faye and Amadou A. Sall, Arbovirus and Viral Hemorrhagic Fevers Unit, Institut Pasteur de Dakar, Dakar, Sénégal, E-mails: ousmane.faye@pasteur.sn and amadou.sall@pasteur.sn. Eva Harris, Division of Infectious Diseases, University of California, Berkeley, Berkeley, CA, E-mail: eharris@berkeley.edu. Benjamin A. Pinsky, Department of Medicine–Infectious Diseases, Stanford University, Stanford, CA, E-mail: bpinsky@stanford.edu. Jesse J. Waggoner, Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, and Department of Global Health, Emory University School of Public Health, Atlanta, GA, E-mail: jesse.j.waggoner@emory.edu.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Monath TP, Vasconcelos PF, 2015. Yellow fever. J Clin Virol 64: 160–173.
-
2.
Dexheimer Paploski IA et al. 2017. Epizootic outbreak of yellow fever virus and risk for human disease in Salvador, Brazil. Ann Intern Med 168: 301–302.
-
3.
World Health Organization, 2017. Yellow Fever Outbreak Angola, Democratic Republic of the Congo and Uganda 2016–2017. Geneva, Switzerland: World Health Organization. Available at: http://www.who.int/emergencies/yellow-fever/en/. Accessed January 8, 2018.
-
4.
Domingo C, Patel P, Yillah J, Weidmann M, Méndez JA, Nakouné ER, Niedrig M, 2012. Advanced yellow fever virus genome detection in point-of-care facilities and reference laboratories. J Clin Microbiol 50: 4054–4060.
-
5.
World Health Organization, 2016. Yellow Fever. Geneva, Switzerland: World Health Organization. Available at: http://www.who.int/mediacentre/factsheets/fs100/en/. Accessed April 4, 2017.
-
6.
Domingo C, Escadafal C, Rumer L, Mendez JA, Garcia P, Sall AA, Teichmann A, Donoso-Mantke O, Niedrig M, 2012. First international external quality assessment study on molecular and serological methods for yellow fever diagnosis. PLoS One 7: e36291.
-
7.
Pan American Health Organization, 2017. Laboratory Diagnosis of Yellow Fever Virus Infection. Washington, DC: Pan American Health Organization.
-
8.
World Health Organization, 2009. Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control. Geneva, Switzerland: WHO Press.
-
9.
Bae HG, Nitsche A, Teichmann A, Biel SS, Niedrig M, 2003. Detection of yellow fever virus: a comparison of quantitative real-time PCR and plaque assay. J Virol Methods 110: 185–191.
-
10.
Nunes MR, Palacios G, Nunes KN, Casseb SM, Martins LC, Quaresma JA, Savji N, Lipkin WI, Vasconcelos PF, 2011. Evaluation of two molecular methods for the detection of yellow fever virus genome. J Virol Methods 174: 29–34.
-
11.
Reusken CBEM, Knoester M, GeurtsvanKessel C, Koopmans M, Knapen DG, Bierman WFW, Pas S, 2017. Urine as sample type for molecular diagnosis of natural yellow fever virus infections. J Clin Microbiol 55: 3294–3296.
-
12.
Waggoner JJ et al. 2013. Development of an internally controlled real-time reverse transcriptase PCR assay for pan-dengue virus detection and comparison of four molecular dengue virus detection assays. J Clin Microbiol 51: 2172–2181.
-
13.
Waggoner JJ et al. 2014. Multiplex nucleic acid amplification test for diagnosis of dengue fever, malaria, and leptospirosis. J Clin Microbiol 52: 2011–2018.
-
14.
Waggoner JJ, Gresh L, Mohamed-Hadley A, Ballesteros G, Davila MJ, Tellez Y, Sahoo MK, Balmaseda A, Harris E, Pinsky BA, 2016. Single-reaction multiplex reverse transcription PCR for detection of zika, chikungunya, and dengue viruses. Emerg Infect Dis 22: 1295–1297.
-
15.
Burd EM, 2010. Validation of laboratory-developed molecular assays for infectious diseases. Clin Microbiol Rev 23: 550–576.
-
16.
Kuan G, Gordon A, Aviles W, Ortega O, Hammond SN, Elizondo D, Nunez A, Coloma J, Balmaseda A, Harris E, 2009. The Nicaraguan pediatric dengue cohort study: study design, methods, use of information technology, and extension to other infectious diseases. Am J Epidemiol 170: 120–129.
-
17.
Waggoner JJ et al. 2013. Comparison of the FDA-approved CDC DENV-1–4 real-time reverse transcription-PCR with a laboratory-developed assay for dengue virus detection and serotyping. J Clin Microbiol 51: 3418–3420.
-
18.
Colebunders R et al. 2002. A Belgian traveler who acquired yellow fever in the Gambia. Clin Infect Dis 35: e113–e116.
-
19.
Shearer FM et al. 2017. Global yellow fever vaccination coverage from 1970 to 2016: an adjusted retrospective analysis. Lancet Infect Dis 17: 1209–1217.
-
20.
Pan American Health Organization/World Health Organization, 2017. Epidemiological Update: Yellow Fever. Washington, DC: PAHO/WHO.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1793 | 1501 | 99 |
| Full Text Views | 628 | 19 | 4 |
| PDF Downloads | 210 | 27 | 6 |