Author:
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-
1.
Bessières M-H, Cassaing S, Fillaux J, Berrebi A, 2008. Toxoplasmose et grossesse. Revue Francophone des Laboratoires 2008: 39–50.
-
2.
Bouratbine A, Siala E, Chahed MK, Aoun K, Ben Ismail R, 2001. Sero-epidemiologic profile of toxoplasmosis in northern Tunisia. Parasite 8: 61–66.
-
3.
Sellami H, Amri H, Cheikhrouhou F, Sellami A, Makni F, Trabelsi H, Trabelsi K, Guermazi M, Ayadi A, 2010. Toxoplasmosis in Sfax, Tunisia. Bull Soc Pathol Exot 103: 37–40.
-
4.
Ben Abdallah R, Siala E, Bouafsoun A, Maatoug R, Souissi O, Aoun K, Bouratbine A, 2013. Toxoplasmosis mother-to-child screening: study of cases followed in the Pasteur Institute of Tunis (2007–2010). Bull Soc Pathol Exot 106: 108–112.
-
5.
Bessières M, Berrebi A, Cassaing S, Fillaux J, Cambus J, Berry A, Assouline C, Ayoubi J, Magnaval J, 2009. Diagnosis of congenital toxoplasmosis: prenatal and neonatal evaluation of methods used in Toulouse University Hospital and incidence of congenital toxoplasmosis. Mem Inst Oswaldo Cruz 104: 389–392.
-
6.
Robert-Gangneux F, Gavinet M-F, Ancelle T, Raymond J, Tourte-Schaefer C, Dupouy-Camet J, 1999. Value of prenatal diagnosis and early postnatal diagnosis of congenital toxoplasmosis: retrospective study of 110 cases. J Clin Microbiol 37: 2893–2898.
-
7.
Villard O, Cimon B, L’Ollivier C, Fricker-Hidalgo H, Godineau N, Houze S, Paris L, Pelloux H, Villena I, Candolfi E, 2016. Serological diagnosis of Toxoplasma gondii infection: recommendations from the French National Reference Center for Toxoplasmosis. Diagn Microbiol Infect Dis 84: 22–33.
-
8.
Machado AS, Andrade GM, Januario JN, Fernandes MD, Carneiro AC, Carneiro M, Carellos EV, Romanelli RM, Vasconcelos-Santos DV, Vitor RW, 2010. IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis. Mem Inst Oswaldo Cruz 105: 757–761.
-
9.
Capobiango JD, Monica TC, Ferreira FP, Mitsuka-Bregano R, Navarro IT, Garcia JL, Reiche EM, 2016. Evaluation of the western blotting method for the diagnosis of congenital toxoplasmosis. J Pediatr (Rio J) 92: 616–623.
-
10.
Gilbert R, Thalib L, Tan H, Paul M, Wallon M, Petersen E, 2007. Screening for congenital toxoplasmosis: accuracy of immunoglobulin M and immunoglobulin A tests after birth. J Med Screen 14: 8–13.
-
11.
Ben Abdallah R, Aoun K, Siala E, Souissi O, Maatoug R, Hlioui S, Bouratbine A, 2009. Congenital toxoplasmosis: clinical and biological analysis of 11 cases in Tunisia. Arch Pediatr 16: 118–121.
-
12.
Pinon JM et al. 2001. Strategy for diagnosis of congenital toxoplasmosis: evaluation of methods comparing mothers and newborns and standard methods for postnatal detection of immunoglobulin G, M, and A antibodies. J Clin Microbiol 39: 2267–2271.
-
13.
Kieffer F, Thulliez P, Kassis M, Rigourd V, Magny J, 2009. Prenatal treatment for congenital toxoplasmosis. Arch Pediatr 16: 885–887.
-
14.
Cortina-Borja M et al. European Multicentre Study on Congenital T, 2010. Prenatal treatment for serious neurological sequelae of congenital toxoplasmosis: an observational prospective cohort study. PLoS Med 7: e1000351.
-
15.
Wallon M, Cozon G, Ecochard R, Lewin P, Peyron F, 2001. Serological rebound in congenital toxoplasmosis: long-term follow-up of 133 children. Eur J Pediatr 160: 534–540.
| Past two years | Past Year | Past 30 Days | |
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Congenital Toxoplasmosis in Tunisia: Prenatal and Neonatal Diagnosis and Postnatal Follow-up of 35 Cases
Yosr Boudaouara
Yosr Boudaouara
Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR11IPT-06, Institute Pasteur of Tunis, Tunis, Tunisia;
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Karim Aoun
Karim Aoun
Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR11IPT-06, Institute Pasteur of Tunis, Tunis, Tunisia;
Department of Parasitology, Institute Pasteur of Tunis, Tunis, Tunisia
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Department of Parasitology, Institute Pasteur of Tunis, Tunis, Tunisia
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Rania Maatoug
Rania Maatoug
Department of Parasitology, Institute Pasteur of Tunis, Tunis, Tunisia
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Olfa Souissi
Olfa Souissi
Department of Parasitology, Institute Pasteur of Tunis, Tunis, Tunisia
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Aïda Bouratbine
Aïda Bouratbine
Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR11IPT-06, Institute Pasteur of Tunis, Tunis, Tunisia;
Department of Parasitology, Institute Pasteur of Tunis, Tunis, Tunisia
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Department of Parasitology, Institute Pasteur of Tunis, Tunis, Tunisia
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Rym Ben Abdallah
Rym Ben Abdallah
Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR11IPT-06, Institute Pasteur of Tunis, Tunis, Tunisia;
Department of Parasitology, Institute Pasteur of Tunis, Tunis, Tunisia
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Department of Parasitology, Institute Pasteur of Tunis, Tunis, Tunisia
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Congenital toxoplasmosis (CT) results from transplacental passage of Toxoplasma gondii to the fetus during acute maternal infection. Our study aims to report clinical and biological patterns of 35 cases of CT diagnosed at the department of the Parasitology of the Pasteur Institute of Tunis and to access the performance of prenatal and early postnatal diagnosis techniques. Serological screening of maternal infection was performed by Immunoglobulin (Ig) M and IgG detection and IgG avidity determination. Prenatal diagnosis was based on both Toxoplasma DNA detection in the amniotic fluid and monthly ultrasound examinations. polymerase chain reaction analysis on amniotic fluid, performed only in 15 cases, detected Toxoplasma’s DNA in five cases (33.3%). Ultrasound examination did not reveal any morphological abnormalities. Thirty newborns had serological criteria of Toxoplasma infection. Congenital toxoplasmosis diagnosis was confirmed in 23 cases (76.6%) by immunoblot. Among the 35 born-infants, five (14.3%) were symptomatic: three had chorioretinitis at the first clinical ocular examination, one had neurological symptoms (seizures) with positive parasite DNA in cerebral spinal fluid, and one had both ophthalmological and neurological damages— chorioretinitis and intracranial calcifications in the computed tomography scan. Thirty-four of 35 infected children were treated with pyrimethamine–sulfadiazine combination. Four (11.7%) of the treated infants showed abnormal hematological values because of the treatment side effect. Serological rebound was observed in seven infants. A screening program and a diagnostic algorithm in pregnant women should be implemented in Tunisia to improve the follow-up of seronegative ones and to prevent CT cases.
Author Notes
Authors’ addresses: Yosr Boudaouara, Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR11IPT-06, Institut Pasteur de Tunis, Tunis, Tunisia, E-mail: boud.yosr@gmail.com. Karim Aoun, Lab of Parasitology, Department of Parasitology, Institut Pasteur de Tunis, Tunis, Tunisia, E-mail: karim.aoun@pasteur.rns.tn. Rania Maatoug, Olfa Souissi, and Aïda Bouratbine, Department of Parasitology, Institut Pasteur de Tunis, Tunis, Tunisia, E-mails: raniamaatoug@gmail.com, souissiolfa75@yahoo.fr, and aida.bouratbine@pasteur.rns.tn. Rym Ben Abdallah, Department of Parasitology, Institut Pasteur de Tunis, Tunis, Tunisia, and Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR11IPT-06, Institut Pasteur de Tunis, Tunis, Tunisia, E-mail: ismail_rym@yahoo.fr.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Bessières M-H, Cassaing S, Fillaux J, Berrebi A, 2008. Toxoplasmose et grossesse. Revue Francophone des Laboratoires 2008: 39–50.
-
2.
Bouratbine A, Siala E, Chahed MK, Aoun K, Ben Ismail R, 2001. Sero-epidemiologic profile of toxoplasmosis in northern Tunisia. Parasite 8: 61–66.
-
3.
Sellami H, Amri H, Cheikhrouhou F, Sellami A, Makni F, Trabelsi H, Trabelsi K, Guermazi M, Ayadi A, 2010. Toxoplasmosis in Sfax, Tunisia. Bull Soc Pathol Exot 103: 37–40.
-
4.
Ben Abdallah R, Siala E, Bouafsoun A, Maatoug R, Souissi O, Aoun K, Bouratbine A, 2013. Toxoplasmosis mother-to-child screening: study of cases followed in the Pasteur Institute of Tunis (2007–2010). Bull Soc Pathol Exot 106: 108–112.
-
5.
Bessières M, Berrebi A, Cassaing S, Fillaux J, Cambus J, Berry A, Assouline C, Ayoubi J, Magnaval J, 2009. Diagnosis of congenital toxoplasmosis: prenatal and neonatal evaluation of methods used in Toulouse University Hospital and incidence of congenital toxoplasmosis. Mem Inst Oswaldo Cruz 104: 389–392.
-
6.
Robert-Gangneux F, Gavinet M-F, Ancelle T, Raymond J, Tourte-Schaefer C, Dupouy-Camet J, 1999. Value of prenatal diagnosis and early postnatal diagnosis of congenital toxoplasmosis: retrospective study of 110 cases. J Clin Microbiol 37: 2893–2898.
-
7.
Villard O, Cimon B, L’Ollivier C, Fricker-Hidalgo H, Godineau N, Houze S, Paris L, Pelloux H, Villena I, Candolfi E, 2016. Serological diagnosis of Toxoplasma gondii infection: recommendations from the French National Reference Center for Toxoplasmosis. Diagn Microbiol Infect Dis 84: 22–33.
-
8.
Machado AS, Andrade GM, Januario JN, Fernandes MD, Carneiro AC, Carneiro M, Carellos EV, Romanelli RM, Vasconcelos-Santos DV, Vitor RW, 2010. IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis. Mem Inst Oswaldo Cruz 105: 757–761.
-
9.
Capobiango JD, Monica TC, Ferreira FP, Mitsuka-Bregano R, Navarro IT, Garcia JL, Reiche EM, 2016. Evaluation of the western blotting method for the diagnosis of congenital toxoplasmosis. J Pediatr (Rio J) 92: 616–623.
-
10.
Gilbert R, Thalib L, Tan H, Paul M, Wallon M, Petersen E, 2007. Screening for congenital toxoplasmosis: accuracy of immunoglobulin M and immunoglobulin A tests after birth. J Med Screen 14: 8–13.
-
11.
Ben Abdallah R, Aoun K, Siala E, Souissi O, Maatoug R, Hlioui S, Bouratbine A, 2009. Congenital toxoplasmosis: clinical and biological analysis of 11 cases in Tunisia. Arch Pediatr 16: 118–121.
-
12.
Pinon JM et al. 2001. Strategy for diagnosis of congenital toxoplasmosis: evaluation of methods comparing mothers and newborns and standard methods for postnatal detection of immunoglobulin G, M, and A antibodies. J Clin Microbiol 39: 2267–2271.
-
13.
Kieffer F, Thulliez P, Kassis M, Rigourd V, Magny J, 2009. Prenatal treatment for congenital toxoplasmosis. Arch Pediatr 16: 885–887.
-
14.
Cortina-Borja M et al. European Multicentre Study on Congenital T, 2010. Prenatal treatment for serious neurological sequelae of congenital toxoplasmosis: an observational prospective cohort study. PLoS Med 7: e1000351.
-
15.
Wallon M, Cozon G, Ecochard R, Lewin P, Peyron F, 2001. Serological rebound in congenital toxoplasmosis: long-term follow-up of 133 children. Eur J Pediatr 160: 534–540.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 589 | 335 | 38 |
| Full Text Views | 1179 | 7 | 2 |
| PDF Downloads | 256 | 12 | 4 |