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Use of TaqMan Array Cards to Screen Outbreak Specimens for Causes of Febrile Illness in Tanzania
We describe the deployment of a custom-designed molecular diagnostic TaqMan Array Card (TAC) to screen for 31 bacterial, protozoal, and viral etiologies in blood from outbreaks of acute febrile illness in Tanzania during 2015–2017. On outbreaks notified to the Tanzanian Ministry of Health, epidemiologists were dispatched and specimens were collected, transported to a central national laboratory, and tested by TAC within 2 days. This algorithm streamlined investigation, diagnosed a typhoid outbreak, and excluded dozens of other etiologies. This method is usable in-country and may be incorporated into algorithms for diagnosing outbreaks.
Author Notes
Financial support: This work was supported by the CDC Global Health Security Partner Engagement Cooperative Agreement #U2GGH001688.
Authors’ addresses: Ahmed Abade, Field Epidemiology and Laboratory Training Program, Dar es Salaam, Tanzania, E-mail: ahmedabade@yahoo.com. Rachel B. Eidex and James J. Gibson, Center for Disease Control and Prevention, Atlanta, GA and Dar es Salaam, Tanzania, E-mails: zvd3@cdc.gov and jerry.gibson.sc@gmail.com. Athanasia Maro, Ireen Kiwelu, Buliga Mujaga, and Blandina T. Mmbaga, Kilimanjaro Clinical Research Institute, Moshi, Tanzania, E-mails: athanasia.maro@gmail.com, i.kiwelu@kcri.ac.tz, b.mujaga@gmail.com, and b.mmbaga@kcri.ac.tz. Jean Gratz, Jie Liu, and Eric R. Houpt, University of Virginia, Charlottesville, VA, E-mails: jean.gratz@gmail.com, jl5yj@virginia.edu, and erh6k@virginia.edu. Maria Kelly and Fausta Mosha, National Health Laboratory Quality Assurance and Training Center, Dar es Salaam, Tanzania, E-mails: mariadorcas8@gmail.com and fausta_mosha@yahoo.com.