• 1.

    Garcia HH, Nash TE, Del Brutto OH, 2014. Clinical symptoms, diagnosis, and treatment of neurocysticercosis. Lancet Neurol 13: 12021215.

  • 2.

    Nash TE, Garcia HH, 2011. Diagnosis and treatment of neurocysticercosis. Nat Rev Neurol 7: 584594.

  • 3.

    Bruno E, Bartoloni A, Zammarchi L, Strohmeyer M, Bartalesi F, Bustos JA, Santivanez S, Garcia HH, Nicoletti A, Group CPS, 2013. Epilepsy and neurocysticercosis in Latin America: a systematic review and meta-analysis. PLoS Negl Trop Dis 7: e2480.

    • Search Google Scholar
    • Export Citation
  • 4.

    Carabin H, Ndimubanzi PC, Budke CM, Nguyen H, Qian Y, Cowan LD, Stoner JA, Rainwater E, Dickey M, 2011. Clinical manifestations associated with neurocysticercosis: a systematic review. PLoS Negl Trop Dis 5: e1152.

    • Search Google Scholar
    • Export Citation
  • 5.

    Winkler AS, 2012. Neurocysticercosis in sub-Saharan Africa: a review of prevalence, clinical characteristics, diagnosis, and management. Pathog Glob Health 106: 261274.

    • Search Google Scholar
    • Export Citation
  • 6.

    Coral-Almeida M, Gabriel S, Abatih EN, Praet N, Benitez W, Dorny P, 2015. Taenia solium human cysticercosis: a systematic review of sero-epidemiological data from endemic zones around the world. PLoS Negl Trop Dis 9: e0003919.

    • Search Google Scholar
    • Export Citation
  • 7.

    Mwape KE, Phiri IK, Praet N, Speybroeck N, Muma JB, Dorny P, Gabriel S, 2013. The incidence of human cysticercosis in a rural community of eastern Zambia. PLoS Negl Trop Dis 7: e2142.

    • Search Google Scholar
    • Export Citation
  • 8.

    Braae UC, Saarnak CF, Mukaratirwa S, Devleesschauwer B, Magnussen P, Johansen MV, 2015. Taenia solium taeniosis/cysticercosis and the co-distribution with schistosomiasis in Africa. Parasit Vectors 8: 323.

    • Search Google Scholar
    • Export Citation
  • 9.

    United States Department of State, 2010. Zambia International Religious Freedom Report 2010. Available at: https://www.state.gov/j/drl/rls/irf/2010/148728.htm. Accessed September 3, 2017.

    • Search Google Scholar
    • Export Citation
  • 10.

    Carabin H, Millogo A, Praet N, Hounton S, Tarnagda Z, Ganaba R, Dorny P, Nitiema P, Cowan LD; Evaluation du Fardeau Economique de la Cysticercose Au Burkina F, 2009. Seroprevalence to the antigens of Taenia solium cysticercosis among residents of three villages in Burkina Faso: a cross-sectional study. PLoS Negl Trop Dis 3: e555.

    • Search Google Scholar
    • Export Citation
  • 11.

    Trinies V, Garn JV, Chang HH, Freeman MC, 2016. The impact of a school-based water, sanitation, and hygiene program on absenteeism, diarrhea, and respiratory infection: a matched-control trial in Mali. Am J Trop Med Hyg 94: 14181425.

    • Search Google Scholar
    • Export Citation
  • 12.

    Noh J, Rodriguez S, Lee YM, Handali S, Gonzalez AE, Gilman RH, Tsang VC, Garcia HH, Wilkins PP, 2014. Recombinant protein- and synthetic peptide-based immunoblot test for diagnosis of neurocysticercosis. J Clin Microbiol 52: 14291434.

    • Search Google Scholar
    • Export Citation
  • 13.

    Corstjens PL, de Dood CJ, Priest JW, Tanke HJ, Handali S; Cysticercosis Working Group in Peru, 2014. Feasibility of a lateral flow test for neurocysticercosis using novel up-converting nanomaterials and a lightweight strip analyzer. PLoS Negl Trop Dis 8: e2944.

    • Search Google Scholar
    • Export Citation
  • 14.

    Handali S et al. 2010. Development and evaluation of a magnetic immunochromatographic test to detect Taenia solium, which causes taeniasis and neurocysticercosis in humans. Clin Vaccine Immunol 17: 631637.

    • Search Google Scholar
    • Export Citation
  • 15.

    Handali S et al. 2010. Multiantigen print immunoassay for comparison of diagnostic antigens for Taenia solium cysticercosis and taeniasis. Clin Vaccine Immunol 17: 6872.

    • Search Google Scholar
    • Export Citation
  • 16.

    Goodhew EB, Priest JW, Moss DM, Zhong G, Munoz B, Mkocha H, Martin DL, West SK, Gaydos C, Lammie PJ, 2012. CT694 and pgp3 as serological tools for monitoring trachoma programs. PLoS Negl Trop Dis 6: e1873.

    • Search Google Scholar
    • Export Citation
  • 17.

    Moss DM, Chard AN, Trinies V, Doumbia S, Freeman MC, Lammie PJ, 2017. Serological responses to filarial antigens in Malian children attending elementary schools. Am J Trop Med Hyg 96: 229232.

    • Search Google Scholar
    • Export Citation
  • 18.

    Kulldorf M, 1997. A spatial scan statistic. Commun Stat Theory 26: 14811496.

  • 19.

    Kulldorf MaIMS, Inc., 2015. SaTScanTM v9.4: Software for the Spatial and Space-Time Scan Statistics. Available at: http://www.satscan.org/. Accessed June 12, 2017.

  • 20.

    Coulibaly YI et al. 2015. The impact of six annual rounds of mass drug administration on Wuchereria bancrofti infections in humans and in mosquitoes in Mali. Am J Trop Med Hyg 93: 356360.

    • Search Google Scholar
    • Export Citation
  • 21.

    Maiga YDM, Bouteille B, Konate A, Diarra M, Maiga M, Marjolet M, 2009. A propos d’un cas autochtone de neurocysticercose au Mali (premier cas de la litterature?). Bull Soc Pathol Exot 102: 211214.

    • Search Google Scholar
    • Export Citation
  • 22.

    Bureau of Democracy HR, and Labor, 2010. International Religious Freedom Report, 2010. Available at: https://www.state.gov/j/drl/rls/irf/2010/148665.htm. Accessed October 10, 2017.

  • 23.

    (OCHA) UNOftCoHA, 2013. Mali: Complex Emergency, Situation Report No. 39. Available at: https://reliefweb.int/sites/reliefweb.int/files/resources/Sitrep39_EN.pdf. Accessed September 12, 2017.

  • 24.

    Kellar KL, Kalwar RR, Dubois KA, Crouse D, Chafin WD, Kane BE, 2001. Multiplexed fluorescent bead-based immunoassays for quantitation of human cytokines in serum and culture supernatants. Cytometry 45: 2736.

    • Search Google Scholar
    • Export Citation
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

 

 

Detection of Immunoglobulin G Antibodies to Taenia solium Cysticercosis Antigen Glutathione-S-Transferase–rT24H in Malian Children Using Multiplex Bead Assay

View More View Less
  • 1 Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia;
  • | 2 Division of Parasitic Diseases and Malaria, Centers for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia;
  • | 3 Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, Georgia;
  • | 4 Division Of Toxicology And Human Health Sciences, Geospatial Research, Analysis And Services Program, Centers for Disease Control and Prevention, Atlanta, Georgia;
  • | 5 Malaria Research and Training Center, Faculty of Medicine and Odontostomatology, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali
Restricted access

Blood samples from 805 students attending 42 elementary schools in Mopti, Sikasso, and Koulikoro regions, and Bamako district in Mali participated in a school water, sanitation, and hygiene intervention. Immunoglobulin (Ig) G responses to several antigens/pathogens were assessed by a multiplex bead assay (MBA), and the recombinant Taenia solium T24H antigen was included. Of all students tested, 8.0% were positive to rT24H, but in some schools 25–30%. A cluster of 12 widespread school locations showed not only a relative risk of 3.23 for T. solium exposure and significantly higher IgG responses (P < 0.001) but also significantly lower elevation (P = 0.04) (m, above sea level) compared with schools outside the cluster. All schools at elevations < 425 m showed significantly higher IgG responses (P = 0.017) than schools at elevations ≥ 425 m. The MBA is an excellent serological platform that provides cost-effective opportunities to expand testing in serosurveys.

Author Notes

Address correspondence to Delynn M. Moss, Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, E-mail: dmm3@cdc.gov or Sukwan Handali, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, 1600 Clifton Rd., Atlanta, GA 30329, E-mail: ahi0@cdc.gov.

Financial support: Funding for this study was provided by Dubai Cares Foundation (DCF), and DCF had no involvement in the study design, sample collection, analysis, or interpretation of the data.

Authors’ addresses: Delynn M. Moss, Waterborne Disease Prevention Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, E-mail: dmm3@cdc.gov. Sukwan Handali, Ryan E. Wiegand, and Patrick J. Lammie, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, GA, E-mails: ahi0@cdc.gov, fwk2@cdc.gov, and pjl1@cdc.gov. Anna N. Chard, Victoria Trinies, and Matthew C. Freeman, Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, E-mails: achard@emory.edu, vtrinies@gmail.com, and mcfreem@emory.edu. Stevan Bullard, Geospatial Research, Analysis and Services Program, Division of Toxicology and Human Health Sciences, Centers for Disease Control and Prevention, Atlanta, GA, E-mail: asz3@cdc.gov. Seydou Doumbia, Faculty of Medicine and Odontostomatology, Malaria Research and Training Center, University of Sciences, Techniques and Technologies of Bamako, E-mail: sdoumbi@icermali.org.

Save