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Natural Infection of Phlebotomus sergenti by Leishmania tropica in Libya

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  • 1 Libyan National Centre for Disease Control (NCDC), Tripoli, Libya;
  • | 2 Department of Zoology, Faculty of Science, University of Sabratha, Sabratha, Libya;
  • | 3 Laboratory of Medical Epidemiology, Institut Pasteur de Tunis, Tunis, Tunisia;
  • | 4 Laboratory of Transmission, Control and Immunobiology of Infections (LR11IPT02), Institut Pasteur de Tunis, Tunis, Tunisia;
  • | 5 Center of Excellence for Emerging and Zoonotic Animal Disease, Kansas State University, Manhattan, Kansas;
  • | 6 Faculty of Medicine of Tunis, University Tunis El Manar, Tunis, Tunisia;
  • | 7 Department of Family and Community Medicine, College of Medicine and Medical Sciences (CMMS), Arabian Gulf University (AGU), Manama, Bahrain;
  • | 8 Public Health Department, Faculty of Medical Technology, University of Tripoli, Tripoli, Libya
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Cutaneous Leishmaniasis (CL) is a public health concern caused by Leishmania (L.) major and L. tropica in Libya. Information on sandfly vectors, as well as their associated Leishmania species, is of paramount importance because vector dispersion is one of the major factors responsible for pathogen dissemination. A number of 515 sandflies (275 males and 240 females) were collected during June–November 2012 using the Centers for Disease Control miniature light traps from Al Rabta, northwest of Libya. Two hundred and forty unfed females were identified; Phlebotomus (Ph.) papatasi (N = 97), Ph. sergenti (N = 27), Ph. longicuspis (N = 32), Sergentomyia (Se.) minuta (N = 38), and Se. fallax (N = 46). These flies were screened for Leishmania DNA using the polymerase chain reaction–restriction fragment length polymorphism analysis of the internal transcribed spacer 1 and sequencing. Two Ph. sergenti were found positive to L. tropica DNA. This finding should be considered for any further vector surveillance and epidemiological studies of CL in endemic areas across Libya.

Author Notes

Address correspondence to Kaouther Jaouadi, Laboratory of Medical Epidemiology, Institut Pasteur de Tunis, 13 Place Pasteur, BP 74 1002, Tunis-Belvédère, Tunisia. E-mail: kaouther.jaouadi@pasteur.tn

Financial support: This study is supported by the Libyan National Centre for Disease Control (NCDC), Tripoli, Libya, and the Laboratory of Medical Epidemiology, Institut Pasteur de Tunis, Tunisia.

Authors’ addresses: Mostafa Ramadhan Dokhan, Libyan National Centre for Disease Control (NCDC), Tripoli, Libya, and Department of Zoology, Faculty of Science, University of Sabratha, Sabratha, Libya, E-mail: mustsun@yahoo.com. Kaouther Jaouadi and Sadok Salem, Laboratory of Medical Epidemiology, Institut Pasteur de Tunis, Tunis, Tunisia, and Laboratory of Transmission, Control and Immunobiology of Infections (LR11IPT02), Institut Pasteur de Tunis, Tunis, Tunisia, E-mails: kaouther.jaouadi@pasteur.tn and sadok-salem@live.fr. Osama Zenbil, Libyan National Centre for Disease Control (NCDC), Tripoli, Libya, E-mail: oys2016.ly@gmail.com. Jean Paul Gonzalez, Center of Excellence for Emerging and Zoonotic Animal Disease, Kansas State University, Manhattan, KS, E-mail: jpgonzalez2808@gmail.com. Afif Ben Salah, Laboratory of Medical Epidemiology, Institut Pasteur de Tunis, Tunis, Tunisia, Laboratory of Transmission, Control and Immunobiology of Infections (LR11IPT02), Institut Pasteur de Tunis, Tunis, Tunisia, Faculty of Medicine of Tunis, University Tunis El Manar, Tunis, Tunisia, and Department of Family and Community Medicine, College of Medicine and Medical Sciences (CMMS), Arabian Gulf University (AGU), Manama, Bahrain, E-mail: afif.bensalah@pasteur.tn. Badreddin Bashir Annajar, Libyan National Centre for Disease Control (NCDC), Tripoli, Libya, and Public Health Department, Faculty of Medical Technology, University of Tripoli, Tripoli, Libya, E-mail: bbannajar@yahoo.com.

These authors contributed equally to this work.

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