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Assessment of Real-Time Polymerase Chain Reaction for the Detection of Trichostrongylus spp. DNA from Human Fecal Samples

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  • 1 Centro per le Malattie Tropicali, Ospedale Sacro Cuore-Don Calabria, Verona, Italy

Sporadic cases of Tricostrongylosis are reported in humans. Diagnosis of enteric Trichostrongylus relies primarily on coproscopic analysis but morphological identification is difficult because of similarity among nematode species. The method is time consuming and requires some expertise. To overcome these limitations, we developed a molecular approach by real-time polymerase chain reaction (PCR) to provide a rapid, specific, and sensitive tool to detect Trichostrongylus spp. in human feces. We designed primers and probe specific for Trichostrongylus rDNA region 5.8S and internal transcribed spacer 2. Three Italian family clusters were analyzed and DNA sequencing was performed to confirm real-time PCR results comparing with known GenBank sequence data. Sequence analysis showed ≥ 99% identity to Trichostrongylus colubriformis and Trichostrongylus axei. This study provides a molecular methodology suitable for fast and specific detection of Trichostrongylus in fecal specimens and to distinguish the zoonotic species.

    • Supplementary Materials

Author Notes

Address correspondence to Francesca Perandin or Elena Pomari, Centro per le Malattie Tropicali, Ospedale Classificato Equiparato, “Sacro Cuore-Don Calabria,” Presidio Ospedaliero Accreditato-Regione Veneto, Via Don A. Sempreboni, 5, I-37024 Negrar, Verona, Italy. E-mails: francesca.perandin@sacrocuore.it and elena.pomari@sacrocuore.it

Authors’ addresses: Francesca Perandin, Elena Pomari, Camilla Bonizzi, Manuela Mistretta, Fabio Formenti, and Zeno Bisoffi, Centro per le Malattie Tropicali, Ospedale Sacro Cuore-Don Calabria Negrar, Verona, Italy, E-mails: francesca.perandin@sacrocuore.it, elena.pomari@sacrocuore.it, camilla.bonizzi@gmail.com, manuela.mistretta@sacrocuore.it, fabio.formenti@sacrocuore.it, and zeno.bisoffi@sacrocuore.it.

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