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-
1.
Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, den Boer M; WHO Leishmaniasis Control Team, 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS One 7: e35671.
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2.
Karunaweera ND, Rajapaksa US, 2009. Is leishmaniasis in Sri Lanka benign and be ignored? J Vector Borne Dis 46: 13–17.
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Siriwardana H, Chandrawansa PH, Sirimanna G, Karunaweera ND, 2012. Leishmaniasis in Sri Lanka: a decade old story. Sri Lanka J Infect Dis 2: 2–12.
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de Vries HJC, Reedijk SH, Schallig HDFH, 2015. Cutaneous leishmaniasis: recent developments in diagnosis and management. Am J Clin Dermatol 16: 99–109.
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5.
Aronson N, Herwaldt BL, Libman M, Pearson R, Lopez-Velez R, Weina P, Carvalho EM, Ephros M, Jeronimo S, Magill A, 2016. Diagnosis and treatment of leishmaniasis: clinical practice guidelines by the Infectious Diseases Society of America (IDSA) and the American Society of Tropical Medicine and Hygiene (ASTMH). Clin Infect Dis 63: 1539–1557.
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6.
Ranawaka RR, Abeygunasekara PH, Weerakoon HS, 2012. Correlation of clinical, parasitological and histopathological diagnosis of cutaneous leishmaniasis in an endemic region in Sri Lanka. Ceylon Med J 57: 149–152.
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7.
Center for Disease Control and Prevention, 2011. Practical Guide for Laboratory Diagnosis of Leishmaniasis. Available at: https://www.cdc.gov/parasites/leishmaniasis/health_professionals/index.html#dx. Accessed March 15, 2013.
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8.
World Health Organization, 2010. Control of the leishmaniases. World Health Organ Tech Rep Ser 949: 22–26. Available at: http://www.who.int/leishmaniasis/resources/technical_report_series/en/. Accessed June 30, 2017.
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9.
Kaul N, Gupta V, Bhardwaj S, Dogra D, Dogra N, 2016. A new focus of cutaneous leishmaniasis in Jammu division of Jammu and Kashmir State, India. Indian J Dermatol Venereol Leprol 82: 145–150.
-
10.
Al-hucheimi SN, Sultan BA, Al-Dhalimi MA, 2009. A comparative study of the diagnosis of Old World cutaneous leishmaniasis in Iraq by polymerase chain reaction and microbiologic and histopathologic methods. Int J Dermatol 48: 404–408.
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11.
ul Bari A, ber Rahman S, 2006. Correlation of clinical, histopathological, and microbiological findings in 60 cases of cutaneous leishmaniasis. Indian J Dermatol Venereol Leprol 72: 28–32.
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Reed SG, 1996. Diagnosis of leishmaniasis. Clin Dermatol 14: 471–478.
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13.
Ramírez JR, Agudelo S, Muskus C, Alzate JF, Berberich C, Barker D, Velez ID, 2000. Diagnosis of cutaneous leishmaniasis in Colombia: the sampling site within lesions influences the sensitivity of parasitologic diagnosis. J Clin Microbiol 38: 3768–3773.
-
14.
Bahamdan KA, Khan AR, Tallab TM, Mourad MM, 1996. Value of touch preparations (imprints) for diagnosis of cutaneous leishmaniasis. Int J Dermatol 35: 558–560.
-
15.
Sousa AQ, Pompeu MML, Frutuoso MS, Lima JWO, Tinel JMBM, Pearson RD, 2014. Short report: press imprint smear: a rapid, simple, and cheap method for the diagnosis of cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis. Am J Trop Med Hyg 91: 905–907.
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Kirstein OD, Abbasi I, Horwitz BZ, Skrip L, Hailu A, Jaffe C, Li LL, Prow TW, Warburg A, 2017. Minimally invasive microbiopsies: a novel sampling method for identifying asymptomatic, potentially infectious carriers of Leishmania donovani. Int J Parasitol 47: 609–616.
-
17.
de Mello CX, Madeira M de F, 2015. Skin scraping is the most accessible technique for the parasitological diagnosis of American tegumentary leishmaniasis. Am J Trop Med Hyg 92: 1082.
-
18.
Berger RS, Perez-Figaredo RA, Spielvogel RL, 1987. Leishmaniasis: the touch preparation as a rapid means of diagnosis. J Am Acad Dermatol 16: 1096–1105.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 499 | 363 | 13 |
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Tissue Impression Smears as a Supplementary Diagnostic Method for Histopathology in Cutaneous Leishmaniasis in Sri Lanka
Nuwani H. Manamperi
Nuwani H. Manamperi
Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka;
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M.V. Chandu de Silva
M.V. Chandu de Silva
Department of Pathology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka;
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Nishantha Pathirana
Nishantha Pathirana
Department of Dermatology, Army Hospital, Colombo, Sri Lanka;
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Wimal Abeyewickreme
Wimal Abeyewickreme
Department of Parasitology, Faculty of Medicine, General Sir John Kotelawala Defence University, Rathmalana, Sri Lanka;
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Nadira D. Karunaweera
Nadira D. Karunaweera
Department of Parasitology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka
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Cutaneous leishmaniasis (CL) is diagnosed mainly by light microscopy of smears made using lesion material. Histopathology is usually done in atypical presentations or when lesion smears are negative. Tissue impression smears (TIS) made from skin biopsy specimens were compared with histopathology for the diagnosis of CL. Out of the 111 patients included, 83 (74.8%) were positive by either methods. The TIS was positive in 70.3% whereas histopathology was positive in 56.8% of patients. Tissue impression smears can be used as a supplementary diagnostic test that gives sensitive and rapid results when tissue biopsies are used as the source of lesion material for diagnosis of CL.
Author Notes
Financial support: Research reported in this publication was supported by the National Institute of Allergy And Infectious Diseases of the National Institutes of Health under Award Numbers R01AI099602 and U01AI136033, the University Grants Commission of Sri Lanka (research grant: UGC/VC/DRIC/PG/2013/KLN/03), and University of Kelaniya, Sri Lanka (research grant: RP/03/04/06/01/2014).
The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health or any other funding agency.
Authors’ addresses: Nuwani H. Manamperi, Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka, E-mail: nuwani_harsh@kln.ac.lk. M.V. Chandu de Silva, Department of Pathology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka, E-mail: desilva_vipula@yahoo.com. Nishantha Pathirana, Department of Dermatology, Army Hospital, Colombo, Sri Lanka, E-mail: kpnpathirana@yahoo.com. Wimal Abeyewickreme, Department of Parasitology, Faculty of Medicine, General Sir John Kotelawala Defence University, Rathmalana, Sri Lanka, E-mail: wabeyewickreme@yahoo.com. Nadira D. Karunaweera, Department of Parasitology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka, E-mail: nadira@parasit.cmb.ac.lk.
ProCite
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Reference Manager
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-
1.
Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, den Boer M; WHO Leishmaniasis Control Team, 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS One 7: e35671.
-
2.
Karunaweera ND, Rajapaksa US, 2009. Is leishmaniasis in Sri Lanka benign and be ignored? J Vector Borne Dis 46: 13–17.
-
3.
Siriwardana H, Chandrawansa PH, Sirimanna G, Karunaweera ND, 2012. Leishmaniasis in Sri Lanka: a decade old story. Sri Lanka J Infect Dis 2: 2–12.
-
4.
de Vries HJC, Reedijk SH, Schallig HDFH, 2015. Cutaneous leishmaniasis: recent developments in diagnosis and management. Am J Clin Dermatol 16: 99–109.
-
5.
Aronson N, Herwaldt BL, Libman M, Pearson R, Lopez-Velez R, Weina P, Carvalho EM, Ephros M, Jeronimo S, Magill A, 2016. Diagnosis and treatment of leishmaniasis: clinical practice guidelines by the Infectious Diseases Society of America (IDSA) and the American Society of Tropical Medicine and Hygiene (ASTMH). Clin Infect Dis 63: 1539–1557.
-
6.
Ranawaka RR, Abeygunasekara PH, Weerakoon HS, 2012. Correlation of clinical, parasitological and histopathological diagnosis of cutaneous leishmaniasis in an endemic region in Sri Lanka. Ceylon Med J 57: 149–152.
-
7.
Center for Disease Control and Prevention, 2011. Practical Guide for Laboratory Diagnosis of Leishmaniasis. Available at: https://www.cdc.gov/parasites/leishmaniasis/health_professionals/index.html#dx. Accessed March 15, 2013.
-
8.
World Health Organization, 2010. Control of the leishmaniases. World Health Organ Tech Rep Ser 949: 22–26. Available at: http://www.who.int/leishmaniasis/resources/technical_report_series/en/. Accessed June 30, 2017.
-
9.
Kaul N, Gupta V, Bhardwaj S, Dogra D, Dogra N, 2016. A new focus of cutaneous leishmaniasis in Jammu division of Jammu and Kashmir State, India. Indian J Dermatol Venereol Leprol 82: 145–150.
-
10.
Al-hucheimi SN, Sultan BA, Al-Dhalimi MA, 2009. A comparative study of the diagnosis of Old World cutaneous leishmaniasis in Iraq by polymerase chain reaction and microbiologic and histopathologic methods. Int J Dermatol 48: 404–408.
-
11.
ul Bari A, ber Rahman S, 2006. Correlation of clinical, histopathological, and microbiological findings in 60 cases of cutaneous leishmaniasis. Indian J Dermatol Venereol Leprol 72: 28–32.
-
12.
Reed SG, 1996. Diagnosis of leishmaniasis. Clin Dermatol 14: 471–478.
-
13.
Ramírez JR, Agudelo S, Muskus C, Alzate JF, Berberich C, Barker D, Velez ID, 2000. Diagnosis of cutaneous leishmaniasis in Colombia: the sampling site within lesions influences the sensitivity of parasitologic diagnosis. J Clin Microbiol 38: 3768–3773.
-
14.
Bahamdan KA, Khan AR, Tallab TM, Mourad MM, 1996. Value of touch preparations (imprints) for diagnosis of cutaneous leishmaniasis. Int J Dermatol 35: 558–560.
-
15.
Sousa AQ, Pompeu MML, Frutuoso MS, Lima JWO, Tinel JMBM, Pearson RD, 2014. Short report: press imprint smear: a rapid, simple, and cheap method for the diagnosis of cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis. Am J Trop Med Hyg 91: 905–907.
-
16.
Kirstein OD, Abbasi I, Horwitz BZ, Skrip L, Hailu A, Jaffe C, Li LL, Prow TW, Warburg A, 2017. Minimally invasive microbiopsies: a novel sampling method for identifying asymptomatic, potentially infectious carriers of Leishmania donovani. Int J Parasitol 47: 609–616.
-
17.
de Mello CX, Madeira M de F, 2015. Skin scraping is the most accessible technique for the parasitological diagnosis of American tegumentary leishmaniasis. Am J Trop Med Hyg 92: 1082.
-
18.
Berger RS, Perez-Figaredo RA, Spielvogel RL, 1987. Leishmaniasis: the touch preparation as a rapid means of diagnosis. J Am Acad Dermatol 16: 1096–1105.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 499 | 363 | 13 |
| Full Text Views | 550 | 4 | 0 |
| PDF Downloads | 163 | 10 | 0 |