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, , , , , , , , , , , , , , , , , and
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1.
World Health Organization, WHO Multidrug Therapy (MDT). Available at: http://www.who.int/lep/mdt/en/. Accessed May 23, 2017.
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2.
Eichelmann K, González González SE, Salas-Alanis JC, Ocampo-Candiani J, 2013. Leprosy. An update: definition, pathogenesis, classification, diagnosis, and treatment. Actas Dermosifiliogr 104: 554–563.
-
3.
Parkash O, 2009. Classification of leprosy into multibacillary and paucibacillary groups: an analysis. FEMS Immunol Med Microbiol 55: 1–5.
-
4.
Suzuki K, Akama T, Kawashima A, Yoshihara A, Yotsu RR, Ishii N, 2012. Current status of leprosy: epidemiology, basic science and clinical perspectives. J Dermatol 39: 121–129.
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5.
Cardona-Castro N, Beltrán-Alzate JC, Manrique-Hernández R, 2008. Survey to identify Mycobacterium leprae-infected household contacts of patients from prevalent regions of leprosy in Colombia. Mem Inst Oswaldo Cruz 103: 332–336.
-
6.
Romero-Montoya M, Beltran-Alzate JC, Cardona-Castro N, 2017. Evaluation and monitoring of Mycobacterium leprae transmission in household contacts of patients with Hansen’s disease in Colombia. PLoS Negl Trop Dis 11: e0005325.
-
7.
Moura MLN et al. 2013. Active surveillance of Hansen’s disease (leprosy): importance for case finding among extra-domiciliary contacts. PLoS Negl Trop Dis 7: e2093.
-
8.
de Moura RS, Calado KL, Oliveira MLW, Bührer-Sékula S, 2008. Leprosy serology using PGL-I: a systematic review. Rev Soc Bras Med Trop 41 (Suppl 2): 11–18.
-
9.
Martinez AN, Talhari C, Moraes MO, Talhari S, 2014. PCR-based techniques for leprosy diagnosis: from the laboratory to the clinic. PLoS Negl Trop Dis 8: e2655.
-
10.
Duthie MS et al. 2007. Use of protein antigens for early serological diagnosis of leprosy. Clin Vaccine Immunol 14: 1400–1408.
-
11.
Duthie M et al. 2014. A rapid ELISA for the diagnosis of MB leprosy based on complementary detection of antibodies against a novel protein-glycolipid conjugate. Diagn Microbiol Infect Dis 79: 233–239.
-
12.
Bossuyt PM et al. STARD Group, 2015. STARD 2015: an updated list of essential items for reporting diagnostic accuracy studies. BMJ 351: h5527.
-
13.
Wright PF, Nilsson E, Van Rooij EM, Lelenta M, Jeggo MH, 1993. Standardisation and validation of enzyme-linked immunosorbent assay techniques for the detection of antibody in infectious disease diagnosis. Rev Sci Tech 12: 435–450.
-
14.
Jhon C, 2001. Methods in Molecular Biology: The ELISA Guidebook, Vol 49. Totowa, NJ: Human Press.
-
15.
Veloza CLA, Carolina WC, Serrano LML, Peñaranda CNR, Antonio HS, 2010. Research biobanks: ethical and legal considerations. Revista Colombiana de Bioética. 5: 121–141.
-
16.
Landry ML, 2016. Immunoglobulin M for acute infection: true or false? Clin Vaccine Immunol 23: 540–545.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 565 | 397 | 118 |
| Full Text Views | 424 | 11 | 2 |
| PDF Downloads | 104 | 13 | 2 |
Comparison of Enzyme-Linked Immunosorbent Assay Using Either Natural Octyl Disaccharide-Leprosy IDRI Diagnostic or Phenolic Glycolipid-I Antigens for the Detection of Leprosy Patients in Colombia
Mónica Muñoz
Mónica Muñoz
Instituto Colombiano de Medicina Tropical–Universidad CES, Sabaneta, Antioquia, Colombia;
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Juan Camilo Beltrán-Alzate
Juan Camilo Beltrán-Alzate
Instituto Colombiano de Medicina Tropical–Universidad CES, Sabaneta, Antioquia, Colombia;
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Malcolm S. Duthie
Malcolm S. Duthie
IDRI, Seattle, Washington;
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Héctor Serrano-Coll
Héctor Serrano-Coll
Escuela de Graduados, Universidad CES, Medellín, Antioquia, Colombia;
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Nora Cardona-Castro
Nora Cardona-Castro
Instituto Colombiano de Medicina Tropical–Universidad CES, Sabaneta, Antioquia, Colombia;
Facultad de Medicina Universidad CES, Medellín, Antioquia, Colombia
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Facultad de Medicina Universidad CES, Medellín, Antioquia, Colombia
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Leprosy is a chronic infectious disease with a broad spectrum of manifestations. Delays in attaining correct diagnosis permit progressive peripheral nerve damage that can produce irreversible disabilities. Tests detecting antigen-specific antibodies can aid the diagnostic process and potentially detect patients earlier. Reported tests have lacked optimal sensitivity and specificity; however, the need to develop new tests to aid early diagnosis still remains. In this study, we determined the sensitivity, specificity, positive predictive value, and negative predictive value of enzyme-linked immunosorbent assay (ELISA) using natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID). Serum samples from confirmed multibacillary patients (N = 338) and paucibacillary patients (N = 58) were evaluated and contrasted against samples from individuals without leprosy (100 healthy persons, 36 leishmaniasis or tuberculosis patients). ELISA detecting either antigen-specific IgM, IgG, or the combination of IgG and IgM (with protein A) were conducted. At a sensitivity of 78% among all patients, serum IgM antibodies against the NDO-LID conjugate were detected at a greater level than those recognizing phenolic glycolipid-I antigen (64% overall sensitivity), while providing similar specificity (97% versus 100%, respectively). Given the inclusion of the LID-1 protein within NDO-LID, we also detected conjugate-specific IgG within patient sera at a sensitivity of 81.6%. The use of protein A to simultaneously detect both antigen-specific IgG and IgM isotypes yielded the highest overall sensitivity of 86.3%. Taken together, our data indicate that the detection of both IgG and IgM antibodies against NDO-LID with protein A provided the best overall ability to detect Colombian leprosy patients.
Author Notes
Authors’ addresses: Mónica Muñoz, Juan Camilo Beltrán-Alzate, and Nora Cardona-Castro, Department of Microbiology, Instituto Colombiano de Medicina tropical–Facultad de Medicina Univerisidad CES, Sabaneta, Antioquia, Colombia, E-mails: monicax15@hotmail.com, jbeltran@ces.edu.co, and ncardona@ces.edu.co. Héctor Serrano-Coll, Escuela de Graduados, Universidad CES, Medellín, Antioquia, Colombia, E-mail: hectorserranocoll@gmail.com. Malcolm S. Duthie, Infectious Disease Research Institute, Diagnostics/Surveillance Tools, Seattle, WA, E-mail: malcolm.duthie@idri.org.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
World Health Organization, WHO Multidrug Therapy (MDT). Available at: http://www.who.int/lep/mdt/en/. Accessed May 23, 2017.
-
2.
Eichelmann K, González González SE, Salas-Alanis JC, Ocampo-Candiani J, 2013. Leprosy. An update: definition, pathogenesis, classification, diagnosis, and treatment. Actas Dermosifiliogr 104: 554–563.
-
3.
Parkash O, 2009. Classification of leprosy into multibacillary and paucibacillary groups: an analysis. FEMS Immunol Med Microbiol 55: 1–5.
-
4.
Suzuki K, Akama T, Kawashima A, Yoshihara A, Yotsu RR, Ishii N, 2012. Current status of leprosy: epidemiology, basic science and clinical perspectives. J Dermatol 39: 121–129.
-
5.
Cardona-Castro N, Beltrán-Alzate JC, Manrique-Hernández R, 2008. Survey to identify Mycobacterium leprae-infected household contacts of patients from prevalent regions of leprosy in Colombia. Mem Inst Oswaldo Cruz 103: 332–336.
-
6.
Romero-Montoya M, Beltran-Alzate JC, Cardona-Castro N, 2017. Evaluation and monitoring of Mycobacterium leprae transmission in household contacts of patients with Hansen’s disease in Colombia. PLoS Negl Trop Dis 11: e0005325.
-
7.
Moura MLN et al. 2013. Active surveillance of Hansen’s disease (leprosy): importance for case finding among extra-domiciliary contacts. PLoS Negl Trop Dis 7: e2093.
-
8.
de Moura RS, Calado KL, Oliveira MLW, Bührer-Sékula S, 2008. Leprosy serology using PGL-I: a systematic review. Rev Soc Bras Med Trop 41 (Suppl 2): 11–18.
-
9.
Martinez AN, Talhari C, Moraes MO, Talhari S, 2014. PCR-based techniques for leprosy diagnosis: from the laboratory to the clinic. PLoS Negl Trop Dis 8: e2655.
-
10.
Duthie MS et al. 2007. Use of protein antigens for early serological diagnosis of leprosy. Clin Vaccine Immunol 14: 1400–1408.
-
11.
Duthie M et al. 2014. A rapid ELISA for the diagnosis of MB leprosy based on complementary detection of antibodies against a novel protein-glycolipid conjugate. Diagn Microbiol Infect Dis 79: 233–239.
-
12.
Bossuyt PM et al. STARD Group, 2015. STARD 2015: an updated list of essential items for reporting diagnostic accuracy studies. BMJ 351: h5527.
-
13.
Wright PF, Nilsson E, Van Rooij EM, Lelenta M, Jeggo MH, 1993. Standardisation and validation of enzyme-linked immunosorbent assay techniques for the detection of antibody in infectious disease diagnosis. Rev Sci Tech 12: 435–450.
-
14.
Jhon C, 2001. Methods in Molecular Biology: The ELISA Guidebook, Vol 49. Totowa, NJ: Human Press.
-
15.
Veloza CLA, Carolina WC, Serrano LML, Peñaranda CNR, Antonio HS, 2010. Research biobanks: ethical and legal considerations. Revista Colombiana de Bioética. 5: 121–141.
-
16.
Landry ML, 2016. Immunoglobulin M for acute infection: true or false? Clin Vaccine Immunol 23: 540–545.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 565 | 397 | 118 |
| Full Text Views | 424 | 11 | 2 |
| PDF Downloads | 104 | 13 | 2 |