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Porcine Cysticercosis: Possible Cross-Reactivity of Taenia hydatigena to GP50 Antigen in the Enzyme-Linked Immunoelectrotransfer Blot Assay
The lentil lectin glycoprotein enzyme-linked immunoelectrotransfer blot (LLGP EITB, reported sensitivity 99% and specificity 100%) is used as a serologic marker of exposure to Taenia solium in pigs. However, only a limited number of parasites have been evaluated for cross reactivity. Pigs may host other related cestode infections, including Taenia hydatigena, which have not been formally evaluated for cross-reactions. We investigated a corral in Tumbes, Peru, a region where a cysticercosis elimination demonstration project was completed in 2012. In this corral, 14/19 (73.7%) 6–8-week-old piglets were reactive to GP50 on LLGP EITB, and all had circulating Taenia sp. antigens. From eight necropsied piglets; four were infected with T. hydatigena metacestodes whereas none had evidence of T. solium infection. Two resident dogs were subsequently confirmed to have T. hydatigena taeniasis. These results suggest GP50 cross-reactivity in T. hydatigena-infected pigs, although controlled experimental infection is needed to confirm this hypothesis.
Author Notes
Financial support: This research was made possible with support from the Fogarty International Center (FIC) and the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) grant number R21AI119438. Partial support from Training grant TW001140 from the Fogarty International Center in training of study team members is also acknowledged.
Authors’ addresses: Claudio Muro and Ricardo Gamboa, Centro de Salud Global Tumbes, Universidad Peruana Cayetano Heredia, Lima, Perú, E-mails: claudio.muro@peruresearch.org and rgamboa@peruresearch.org. Luis A. Gomez-Puerta, Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria, Lima, Peru, E-mail: lucho92@yahoo.com. Robert H. Flecker, Department of Epidemiology, School of Public Health, Oregon Health & Science University and Portland State University, Portland, OR, E-mail: flecker@ohsu.edu. Percy Vilchez Barreto, Centro de Salud Global UPCH, Tumbes, Peru, E-mail: pvilchez@peruresearch.org. Pierre Dorny, Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerpen, Belgium, E-mail: pdorny@itg.be. Victor C. W. Tsang, Georgia State University, Atlanta, GA, E-mail: vct123@gmail.com. Robert H. Gilman, Department of International Health, Johns Hopkins University, Baltimore, MD, E-mail: gilmanbob@gmail.com. Armando E. Gonzalez, School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru, E-mail: agonza41@jhu.edu. Hector H. Garcia, Centro de Salud Global Tumbes, Universidad Peruana Cayetano Heredia, Lima, Peru and Department of Microbiology, School of Sciences, Universidad Peruana Cayetano Heredia, Lima, Peru, E-mail: hgarcia1@jhu.edu. Seth E. O’Neal, Department of Epidemiology, School of Public Health, Oregon Health & Science University and Portland State University, Portland, OR and Center for Global Health - Tumbes, Universidad Peruana Cayetano Heredia, Lima, Peru, E-mail: oneals@ohsu.edu.